Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Cold-Adapted and Rhizosphere-Competent Strain of Rahnella sp. with Broad-Spectrum Plant Growth-Promotion Potential

  • Vyas, Pratibha (Plant Pathology and Microbiology Laboratory, Hill Area Tea Science, Institute of Himalayan Bioresource Technology, Council of Scientific and Industrial Research) ;
  • Joshi, Robin (Biochemistry Laboratory, Hill Area Tea Science, Institute of Himalayan Bioresource Technology, Council of Scientific and Industrial Research) ;
  • Sharma, K.C. (Krishi Vigyan Kendra, CSK Himachal Pradesh Krishi Vishvavidyalaya) ;
  • Rahi, Praveen (Plant Pathology and Microbiology Laboratory, Hill Area Tea Science, Institute of Himalayan Bioresource Technology, Council of Scientific and Industrial Research) ;
  • Gulati, Ashu (Biochemistry Laboratory, Hill Area Tea Science, Institute of Himalayan Bioresource Technology, Council of Scientific and Industrial Research) ;
  • Gulati, Arvind (Plant Pathology and Microbiology Laboratory, Hill Area Tea Science, Institute of Himalayan Bioresource Technology, Council of Scientific and Industrial Research)
  • Received : 2010.07.14
  • Accepted : 2010.09.14
  • Published : 2010.12.28
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Abstract

A phosphate-solubilizing bacterial strain isolated from Hippophae rhamnoides rhizosphere was identified as Rahnella sp. based on its phenotypic features and 16S rRNA gene sequence. The bacterial strain showed the growth characteristics of a cold-adapted psychrotroph, with the multiple plant growth-promoting traits of inorganic and organic phosphate solubilization, 1-aminocyclopropane-1-carboxylate-deaminase activity, ammonia generation, and siderophore production. The strain also produced indole-3-acetic acid, indole-3-acetaldehyde, indole-3-acetamide, indole-3-acetonitrile, indole-3-lactic acid, and indole-3-pyruvic acid in tryptophan-supplemented nutrient broth. Gluconic, citric and isocitric acids were the major organic acids detected during tricalcium phosphate solubilization. A rifampicin-resistant mutant of the strain exhibited high rhizosphere competence without disturbance to the resident microbial populations in pea rhizosphere. Seed bacterization with a charcoal-based inoculum significantly increased growth in barley, chickpea, pea, and maize under the controlled environment. Microplot testing of the inoculum at two different locations in pea also showed significant increase in growth and yield. The attributes of cold-tolerance, high rhizosphere competence, and broad-spectrum plant growth-promoting activity exhibited the potential of Rahnella sp. BIHB 783 for increasing agriculture productivity.

Keywords

References

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