YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Effects of Histidine Polymers on Mucin Release from Primary Cultured Airway Epithelial Cells

히스티딘 중합체가 일차배양 기도 상피세포에서의 뮤신 유리에 미치는 영향

  • 전병규 (대구보건대학 방사선과) ;
  • 윤인대 (인제대학교 의과대학) ;
  • 이재우 (엘지 생명과학) ;
  • 이충재 (충남대학교 의학전문대학원 약리학교실)
  • Received : 2010.07.15
  • Accepted : 2010.08.06
  • Published : 2010.10.31
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Abstract

We investigated whether poly-L-histidine (PLH) significantly affect mucin release from cultured airway epithelial cells. Confluent primary hamster tracheal surface epithelial (HTSE) cells were metabolically radiolabeled with $^$^3H$$glucosamine for 24 hr and chased for 30 min in the presence of PLH to assess the effect on $^3H$-mucin release. PLH 9,850 and PLH 6,700 specifically inhibit mucin release from airway goblet cells without significant cytotoxicity. This finding suggests that poly-L-histidine might function as an airway mucoregulative agent.

Keywords

References

  1. Ellis, E. F. : Asthma in childhood. J. Allergy. Clin. Immunol. 72(suppl), 526 (1985).
  2. Mutschler, E. and Derendorf, H. : Drug actions. CRC press, Inc., Boca Raton, Florida, 410 (1995).
  3. Ko, K. H., Lee, C. J., Shin, C. Y., Jo, M.-J. and Kim, K. C. : Inhibition of mucin release from airway goblet cells by polycationic peptides. Am. J. Physiol. 277(21), L811 (1999).
  4. Kim, K. C., Rearick, J. I., Nettesheim, P. and Jetten, A. M. : Biochemical characterization of mucous glycoproteins synthesized and secreted by hamster tracheal epithelial cells in primary culture. J. Biol. Chem. 260, 4021 (1985).
  5. Kim, K. C., Opaskar-Hincman, H. and Bhaskar, K. R. : Secretions from primary hamster tracheal surface epithelial cells in culture: Mucin-like glycoproteins, proteoglycans, and lipids. Exp. Lung Res. 15, 299 (1989). https://doi.org/10.3109/01902148909087860
  6. Kim, K. C. : Possible requirement of collagen gel substratum for production of mucinlike glycoproteins by primary rabbit tracheal epithelial cells in culture. In Vitro. 21, 617 (1985).
  7. Lee, C. J. : Specificity in the inhibition of mucin release from airway goblet cells by polycationic peptides. J. Appl. Pharmacol. 9(3), 218 (2001).
  8. Lee, C. J., Paik, S. H., Ko, K. H. and Kim, K. C. : Effects of polycationic peptides on mucin release from airway goblet cells : relationship between polymer size and activity. Inflamm. Res. 51, 490 (2002). https://doi.org/10.1007/PL00012417
  9. Elferink, J. G. R. : Changes of plasma membrane permeability in neutrophils treated with polycations. Inflammation. 15(2), 103 (1991). https://doi.org/10.1007/BF00917505
  10. Coyle, A. J., Ackerman, S. J. and Irvin, C. G. : Cationic proteins induce airway hyperresponsiveness dependent on charge interactions. Am. Rev. Respir. Dis. 147, 896 (1993). https://doi.org/10.1164/ajrccm/147.4.896
  11. Gleich, G. J., Flavahan, N. A., Fugisawa, T. and Vanhoutte, P. M. : The eosinophil as a mediator of damage to respiratory epithelium; a model for bronchial hyperreactivity. J. Allergy Clin. Immunol. 81, 776 (1988). https://doi.org/10.1016/0091-6749(88)90931-1
  12. Uchida, D. A., Ackerman, S. J., Coyle, A. J., Larsen, G. L., Weller, P. F., Freed, J. and Irvin, C. G. : The effect of human eosinophil granule major basic protein on airway responsiveness in the rat in vivo. Am. Rev. Respir. Dis. 147, 982 (1993). https://doi.org/10.1164/ajrccm/147.4.982
  13. Wardaw, A. J., Dunnette, S., Gleich, G. J., Collins, J. V. and Kay, A. B. : Eosinophils and mast cells in bronchoalveolar lavage in sujects with mild asthma: relationship to bronchial hyperreactivity. Am. Rev. Respir. Dis. 137, 62 (1988). https://doi.org/10.1164/ajrccm/137.1.62
  14. Hammes, M. and Singh, A. : Effect of polycations on permeability of glomerular epithelial cell monolayer to albumin. J. Lab. Clin. Med. 123, 437 (1994).
  15. Cheng, P. W., Sherman, J. M., Boat, T. E. and Bruce, M. : Quantitation of radiolabeled mucous glycoprteins secreted by tracheal explants. Anal. Biochem. 117, 301 (1981). https://doi.org/10.1016/0003-2697(81)90782-X
  16. Yu, X.-Y., Schofield, B. H., Croxton, T., Takahashi, N., Gabrielson, E. W. and Spannhake, E. W. : Physiologic modulation of bronchial epithelial cell barrier function by polycationic exposure. Am. J. Respir. Cell Mol. Biol. 11, 188 (1994). https://doi.org/10.1165/ajrcmb.11.2.8049079
  17. Frigas, E., Loegering, D. A. and Gleich, G. J. : Cytotoxic effects of the guinea pig eosinophil major basic protein on tracheal epithelium. Lab. Invest. 42, 35 (1980).
  18. Motogima, S., Frigas, E., Loegering, D. A. and Gleich, G. J. : Toxicity of eosinophil cationic proteins for gunea pig tracheal epithelium in vitro. Am. Rev. Respir. Dis. 139, 801 (1989). https://doi.org/10.1164/ajrccm/139.3.801