Experimental and Molecular Medicine

Korean Society for Biochemistry and Molecular Biongy (생화학분자생물학회)
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Baicalein protects HT22 murine hippocampal neuronal cells against endoplasmic reticulum stress-induced apoptosis through inhibition of reactive oxygen species production and CHOP induction

  • Choi, Ji-Hyun (Department of Biochemistry and Molecular Biology School of Medicine Medical Science and Engineering Research Center for Bioreaction to Reactive Oxygen Species Biomedical Science Institute Kyung Hee University) ;
  • Choi, A-Young (Department of Biochemistry and Molecular Biology School of Medicine Medical Science and Engineering Research Center for Bioreaction to Reactive Oxygen Species Biomedical Science Institute Kyung Hee University) ;
  • Yoon, Ha-Na (Department of Biochemistry and Molecular Biology School of Medicine Medical Science and Engineering Research Center for Bioreaction to Reactive Oxygen Species Biomedical Science Institute Kyung Hee University) ;
  • Choe, Won-Chae (Department of Biochemistry and Molecular Biology School of Medicine Medical Science and Engineering Research Center for Bioreaction to Reactive Oxygen Species Biomedical Science Institute Kyung Hee University) ;
  • Yoon, Kyung-Sik (Department of Biochemistry and Molecular Biology School of Medicine Medical Science and Engineering Research Center for Bioreaction to Reactive Oxygen Species Biomedical Science Institute Kyung Hee University) ;
  • Ha, Joo-Hun (Department of Biochemistry and Molecular Biology School of Medicine Medical Science and Engineering Research Center for Bioreaction to Reactive Oxygen Species Biomedical Science Institute Kyung Hee University) ;
  • Yeo, Eui-Ju (Department of Biochemistry Gachon University of Medicine and Science) ;
  • Kang, In-Sug (Department of Biochemistry and Molecular Biology School of Medicine Medical Science and Engineering Research Center for Bioreaction to Reactive Oxygen Species Biomedical Science Institute Kyung Hee University)
  • Accepted : 2010.10.14
  • Published : 2010.12.31
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Abstract

Baicalein is one of the major flavonoids in Scutellaria baicalensis Georgi and possesses various effects, including cytoprotection and anti-inflammation. Because endoplasmic reticulum (ER) stress has been implicated in neurodegenerative diseases, such as Alzheimer's disease, Parkinson's disease, and cerebral ischemia, we investigated the effects of baicalein on apoptotic death of HT22 mouse hippocampal neuronal cells induced by thapsigargin (TG) and brefeldin A (BFA), two representative ER stress inducers. Apoptosis, reactive oxygen species (ROS) production, and mitochondrial membrane potential (MMP) were measured by flow cytometry. Expression level and phosphorylation status of ER stress-associated proteins and activation and cleavage of apoptosis-associated proteins were analyzed by Western blot. Baicalein reduced TG- and BFA-induced apoptosis of HT22 cells and activation and cleavage of apoptosis-associated proteins, such as caspase-12 and -3 and poly(ADP-ribose) polymerase. Baicalein also reduced the TG- and BFA-induced expression of ER stress-associated proteins, including C/EBP homologous protein (CHOP) and glucose-regulated protein 78, the cleavage of X-box binding protein-1 and activating transcription factor $6{\alpha}$, and the phosphorylation of eukaryotic initiation factor-$2{\alpha}$ and mitogen-activated protein kinases, such as p38, JNK, and ERK. Knock-down of CHOP expression by siRNA transfection and specific inhibitors of p38 (SB203580), JNK (SP600125), and ERK (PD98059) as well as anti-oxidant (N-acetylcysteine) reduced TG- or BFA-induced cell death. Baicalein also reduced TG- and BFA-induced ROS accumulation and MMP reduction. Taken together, these results suggest that baicalein could protect HT22 neuronal cells against ER stress-induced apoptosis by reducing CHOP induction as well as ROS accumulation and mitochondrial damage.

Keywords

Acknowledgement

Supported by : Kyung Hee University

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