International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Study on Selective Media for Isolation of Entomopathogenic Fungi

  • Shin, Tae-Young (Department of Agricultural Biology, College of Agriculture Life & Environment Science, Chungbuk National University) ;
  • Choi, Jae-Bang (Department of Agricultural Biology, College of Agriculture Life & Environment Science, Chungbuk National University) ;
  • Bae, Sung-Min (Department of Agricultural Biology, College of Agriculture Life & Environment Science, Chungbuk National University) ;
  • Koo, Hyun-Na (Department of Agricultural Biology, College of Agriculture Life & Environment Science, Chungbuk National University) ;
  • Woo, Soo-Dong (Department of Agricultural Biology, College of Agriculture Life & Environment Science, Chungbuk National University)
  • Received : 2009.12.08
  • Accepted : 2010.02.05
  • Published : 2010.03.31
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Abstract

To determine the optimal isolation conditions of the entomopathogenic fungi from soil, we compared their growth characteristics with non-entomopathogenic fungi on agar media containing various concentrations of cooper (II) chloride ($CuCl_2$) or dodine. The result showed that dodine medium is more selective, and the optimal concentration of dodine is determined with $50{\mu}g$/ml. We could isolate several putative entomopathogenic fungi from soil using this, and identify them using ITS analysis. As a result, 64% fungi were identified as typical entomopathogenic fungi. This selective medium may be useful to the rapid and simple isolation of entomopathogenic fungi from soil.

Keywords

References

  1. Abdo C, Nemer N, Nemer G, Abou Jawdah Y, Kawar N (2008) Isolation of Beauveria species from Lebanon and evaluation of its efficacy against the cedar web-spinning sawfly, Cephalcia tannourinensis. Biocontrol 53, 341-352. https://doi.org/10.1007/s10526-006-9062-0
  2. Beilharz VC, Parberry DG, Swart HJ (1982) Dodine: A selective agent for certain soil fungi. Trans Br Mycol Soc 79, 507-511. https://doi.org/10.1016/S0007-1536(82)80043-0
  3. Brownbridge M, Reay SD, Cummings NJ (2010) Association of entomopathogenic fungi with exotic bark beetles in New Zealand pine plantations. Mycopathologia 169(1), 75-80. https://doi.org/10.1007/s11046-009-9229-1
  4. Chase AR, Osborne LS, Ferguson VM (1986) Selective isolation of the entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae from an artificial potting medium. Flo Entol 69, 285-292. https://doi.org/10.2307/3494930
  5. Da Costa GL, de Moraes AM, de Oliveira PC (1998) Pathogenic action of Penicillium species on mosquito vectors of human tropical diseases. J Basic Microbiol 38(5-6), 337-341. https://doi.org/10.1002/(SICI)1521-4028(199811)38:5/6<337::AID-JOBM337>3.0.CO;2-N
  6. Freimoser FM, Screen S, Bagga S, Hu G, St. Leger RJ (2003) Expressed sequence tag (EST) analysis of two subspecies of Metarhizium anisopliae reveals a plethora of secreted proteins with potential activity in insect hosts. Microbiology 149, 239-247. https://doi.org/10.1099/mic.0.25761-0
  7. Glare TR, Reay SD, Nelson TL, Moore R (2008) Beauveria caledonica is a naturally occurring pathogen of forest beetles. Mycol Res 112(3), 352-360. https://doi.org/10.1016/j.mycres.2007.10.015
  8. Hajek AE, St. Leger RJ (1994) Interactions Between Fungal Pathogens and Insect Hosts. Annu Rev Entomol 39, 293-322. https://doi.org/10.1146/annurev.en.39.010194.001453
  9. Inglis GD, Goettel MS, Butt TM, Strasser H (2001) Use of hyphomycetous fungi for managing insect pests. In: Butt TM, Jackson C, Magan N (eds), Fungi As Biocontrol Agents: Progress, Problems and Potential. CABI Publishing, Wallingford, pp. 23-69.
  10. Keller SP, Kessler P, Schweizer C (2003) Distribution of insect pathogenic soil fungi in Switzerland with special reference to Beauveria brongniartii and Metharhizium anisopliae. Biocontrol 48, 307-319. https://doi.org/10.1023/A:1023646207455
  11. Mark SG, Douglas GI (1997) Fungi: Hyphomycetes. In: Lacey L, ed. Manual of Techniques in Insect Pathology. San Diego, California: Academic Press, London, 53-185.
  12. Meyling NV, Eilenberg J (2006) Isolation and characterisation of Beauveria bassiana isolates from phylloplanes of hedgerow vegetation. Mycological Research 110, 188-195. https://doi.org/10.1016/j.mycres.2005.09.008
  13. Meyling NV (2007) Methods for isolation of entomopathogenic fungi from the soil environment. laboratory manual, 1-18.
  14. Pendland JC, Hung SY, Boucias DG (1993) Evasion of host defense by in vivo-produced protoplast-like cells of the insect mycopathogen Beauveria bassiana. J Bacteriol 175, 5962-5969. https://doi.org/10.1128/jb.175.18.5962-5969.1993
  15. Santoro PH, Neves PM, Alexandre TM, Sartori D, Alves LF, Fungaro MH (2008) Selection of Beauveria bassiana isolates to control Alphitobius diaperinus. J Invertebr Pathol 97(2), 83-90. https://doi.org/10.1016/j.jip.2007.07.009
  16. Shah PA, Pell JK (2003) Entomopathogenic fungi as biological control agents. Appl Microbiol Biotech 61, 413-423. https://doi.org/10.1007/s00253-003-1240-8
  17. Shimazu M, Sato H (1996) Media for selective isolation of an entomogenous fungus, Beauveria bassiana (Deuteromycotina: Hyphomycetes). Appl Entomol Zool 31, 291-298. https://doi.org/10.1303/aez.31.291
  18. Shimazu M, Sato H, Maehara N (2002) Density of the entomopathogenic fungus, Beauveria bassiana Vuillemin (Deuteromycotina: Hyphomycetes) in forest air and soil. Appl Entomol Zool 37(1), 19-26. https://doi.org/10.1303/aez.2002.19
  19. Shin TY, Choi JB, Bae SM, Cha YR, Oh JM, Koo HN, Woo SD (2009) Isolation and Identification of Entomopathogenic Fungus from the Pine Wilt Disease Vector, Monochamus alternatus Hope (Coleoptera: Cerambycidae) in Korea. Int J Indust Entomol 18(2), 125-129.
  20. Srivastava PP, Bansal AK, Shukla RM, Banerjee ND, Saxena NN, Sinha SS (1994) Utilization of cuticular components of Antheraea mylitta Drury larvae by Penicillium citrinum Thom. Mycopathologia 128, 81-84. https://doi.org/10.1007/BF01103013
  21. St. Leger RJ, Wang C (2009) Entomopathonic Fungi and the Genomics Era. In: Patricia Stock SP, Vanderberg J, Boemare N, Glazer I, eds. Insect Pathogens: Molecular Approaches and Techniques, CABI. pp. 365-400.
  22. Tscharntke T, Klein AM, Kruess A, Steffan-Dewenter I, Thies C (2005) Landscape perspectives on agricultural intensification and biodiversity - ecosystem service management. Ecol Lett 8, 857-874. https://doi.org/10.1111/j.1461-0248.2005.00782.x
  23. Veen KH, Ferron P (1966) A selective medium for isolation of Beauveria tenella and of Metarrhizium anisopliae. J Invertebr Pathol 8, 268-269. https://doi.org/10.1016/0022-2011(66)90145-5
  24. Vu VH, Hong SI, Kim K (2007) Selection of entomopathogenic fungi for aphid control. J Biosci Bioeng 104(6), 498-505. https://doi.org/10.1263/jbb.104.498
  25. White TJ, Bruns T, Lee S, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, editor. PCR Protocols: A Guide to Methods and Applications. New York: Academic Press Inc. pp. 315-322.
  26. Zimmermann G (1986) The Galleria bait method for detection of entomopathogenic fungi in soil. Appl Entomol Zool 102, 213-215. https://doi.org/10.1111/j.1439-0418.1986.tb00912.x
  27. Zimmermann G (1998) Suggestion for a standardized method for reisolation of entomopathogenic fungi from soil using the bait method. "Insect pathogens and insect parasitic nematodes". IOBC Bull 21(4), 289.