Restorative Dentistry and Endodontics

The Korean Academy of Conservative Dentistry (대한치과보존학회)
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THE REMINERALIZATION ASPECT OF ENAMEL ACCORDING TO CHANGE OF THE DEGREE OF SATURATION OF THE ORGANIC ACID BUFFERING SOLUTION IN pH 5.5

pH 5.5에서 유기산 완충용액의 포화도 변화에 따른 법랑질의 재광화 양상

  • Park, Jin-Sung (Department of Dentistry, the Graduate School, Yonsei University) ;
  • Park, Sung-Ho (Department of Dentistry, the Graduate School, Yonsei University) ;
  • Park, Jeong-Won (Department of Dentistry, the Graduate School, Yonsei University) ;
  • Lee, Chan-Young (Department of Dentistry, the Graduate School, Yonsei University)
  • 박진성 (연세대학교 치과대학 치과보존학교실) ;
  • 박성호 (연세대학교 치과대학 치과보존학교실) ;
  • 박정원 (연세대학교 치과대학 치과보존학교실) ;
  • 이찬영 (연세대학교 치과대학 치과보존학교실)
  • Received : 2009.12.28
  • Accepted : 2010.03.11
  • Published : 2010.03.31
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Abstract

The purpose of this study is to observe and compare the remineralization tendencies of artificial enamel caries lesion by remineralization solutions of different degree of saturations at pH 5.5, using a polarizing microscope and computer programs (Photoshop, Image pro plus, Scion Image, Excel). For this study, 48 sound permanent teeth with no signs of demineralization, cracks, or dental restorations were used. The specimens were immersed in lactic acid demineralization solution for 2 days in order to produce artificial dental caries that consist of surface and subsurface lesions. Each of 9 or 10 specimens was immersed in pH 5.5 lactic acid buffering remineralization solution of four different degrees of saturation (0.507, 0.394, 0.301, and 0.251) for 12 days. After the demineralization and remineralization, images were taken by a polarizing microscope (${\times}100$). The results were obtained by observing images of the specimens, and using computer programs, the density of caries lesions were est mated. While the group with the lowest degree of saturation (0.251) showed total remineralization feature from the surface to the subsurface of the lesion, the group with the highest degree of saturation (0.507) showed demineralization mainly on the surface of the lesion at the constant organic acid concentration 0.01 M and pH 5.5.

본 연구에서는 lactic acid를 이용하여 인공 탈회용액을 제조하여 우식이 없는 치아로 시편을 만들고 법랑질 부위에 인공 우식 병소를 생성 시키고 유기산 농도 0.01 M, pH 5.5로 고정한 상태에서 유기산 완충용액의 포화도를 달리하여(0.507, 0.394, 0.301, 0.251) 재광화를 시키고 편광 현미경하에서 관찰 및 디지털 카메라로 촬영하였다. 이를 통해 얻은 상에서 탈회 깊이의 변화, 우식 표면층 폭의 변화, 무기질의 양적 변화를 컴퓨터 프로그램을 이용하여 비교, 통계 분석하여 다음 결과를 얻었다. 1. 재광화 전에 비하여 재광화 후 모든 군에서 10-30% 재광화 양이 증가 하였다. 2. 재광화 후 4군에서는 우식 표면층과 심부 모두에서 재광화가 일어나는 양상이 나타난 반면 1군에서는 심부 보다 우식 표면층 부위의 재광화가 더 일어난 양상이 관찰되었다. 3. 재광화 후 시편의 탈회된 깊이의 변화는 탈회시킨 후와 비교할 때 모든 군에서 유의할만한 변화는 없었으며 군간의 통계적 유의차도 없었다. 결론적으로, 유기산 0.01 M, pH 5.5로 고정한 상태에서 포화도가 가장 낮았던 군(0.251)에서는 우식 표면층에서 심부까지 재광화가 일어난 반면, 포화도가 가장 높았던 군(0.507)에서는 우식 표면층에서 재광화가 주로 일어났다.

Keywords

References

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