Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation and Characteristics of Alginate-Degrading Methylobacterium sp. HJM27

알긴산 분해 Methylobacterium sp. HJM27 균주의 분리 및 특성

  • Kim, Ok-Ju (Department of Bioscience and Biotechnology, Graduate School, Silla University) ;
  • Lee, Dong-Geun (Department of Bioscience and Biotechnology, Graduate School, Silla University) ;
  • Lee, Sung-Mok (Department of Bioscience and Biotechnology, Graduate School, Silla University) ;
  • Lee, Suck-June (Department of Bioscience and Biotechnology, Graduate School, Silla University) ;
  • Do, Hyung-Joo (Department of Bioscience and Biotechnology, Graduate School, Silla University) ;
  • Park, Hye-Jin (Department of Pharmaceutical Engineering, College of Medical & Life Science, Silla University) ;
  • Kim, Andre (Department of Pharmaceutical Engineering, College of Medical & Life Science, Silla University) ;
  • Lee, Jae-Hwa (Department of Bioscience and Biotechnology, Graduate School, Silla University) ;
  • Ha, Jong-Myung (Department of Bioscience and Biotechnology, Graduate School, Silla University)
  • 김옥주 (신라대학교 대학원 생명공학과) ;
  • 이동근 (신라대학교 대학원 생명공학과) ;
  • 이성목 (신라대학교 대학원 생명공학과) ;
  • 이석준 (신라대학교 대학원 생명공학과) ;
  • 도형주 (신라대학교 대학원 생명공학과) ;
  • 박혜진 (신라대학교 의생명과학대학 제약공학과) ;
  • 김안드레 (신라대학교 의생명과학대학 제약공학과) ;
  • 이재화 (신라대학교 대학원 생명공학과) ;
  • 하종명 (신라대학교 대학원 생명공학과)
  • Received : 2010.04.22
  • Accepted : 2010.06.07
  • Published : 2010.06.28
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Abstract

This study was aimed to screen bacteria of high alginate-degrading activity, to select the nitrogen source and concentration of NaCl and sodium alginate for the production of alginate-degrading enzyme, and to determine reaction conditions of enzyme. A novel alginate-degrading bacterium was isolated from abalone (Haliotis discus hannai) and named Methylobacterium sp. HJM27 by 16S rDNA sequence analysis. The optimum culture conditions for the production of alginate-degrading enzyme were 1.0% sodium alginate, 0.5% peptone, 0.3% yeast extract, 1.5% NaCl, $25^{\circ}C$ and 48 hours incubation time. The raw enzyme showed the highest activity at $25^{\circ}C$ and pH 9, and produced 1.217 g - reducing sugar per liter in 0.8% (w/v) sodium alginate for 30 minutes. Methylobacterium sp. HJM27 and its alginate-degrading enzyme would be useful for the production of bioenergy and biofunctional oligosaccharides from seaweed.

본 연구는 알긴산 분해효소 활성이 뛰어난 세균을 확보하고 선정된 균주의 효소생산과 활성에 영향을 미치는 알긴산과 NaCl의 농도, 질소원 종류, 온도, pH 등을 파악하고자 하였다. 해조류 섭식 동물인 전복, 소라, 해삼, 멍게, 개불 등에서 유래한 총 5만여 콜로니 중 알긴산 분해효소 활성이 우수한 27개 균주를 분리하였고 최종적으로 전복 유래의 균주를 선정하였다. 16S rDNA 염기서열 분석으로 선정된 균주를 Methylobacterium sp. HJM27으로 명명하였고 알긴산 분해효소의 활성은 1.0% sodium alginate, 0.5% peptone, 0.3% yeast extract, 1.5% NaCl, $25^{\circ}C$, 48시간 배양에서 가장 높았다. 알긴산 분해효소의 활성은 $25^{\circ}C$, pH 9에서 최대로 0.8%(w/v) sodium alginate 용액에서 30분만에 1.217 g/L의 환원당을 생성하였다. 분리된 균주와 이 균주의 알긴산 분해효소를 이용하여 해조류를 바이오에너지와 식 의약 소재로 활용 할 수 있을 것으로 판단된다.

Keywords

References

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