The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
권호 표지

Effects of Goihwa-san on Ulcerative Colitis Induced by Dextran Sulfate Sodium in Mice

괴화산(槐花散)이 Dextran Sulfate Sodium으로 유도된 생쥐의 궤양성 대장염에 미치는 영향

  • Bae, Kwang-Ho (Dept. of Internal Medicine, Oriental Medical College, Se-Myung University) ;
  • Kong, Kyung-Hwan (Dept. of Internal Medicine, Oriental Medical College, Se-Myung University)
  • 배광호 (세명대학교 한의과대학 부속 충주한방병원 내과학교실) ;
  • 공경환 (세명대학교 한의과대학 부속 충주한방병원 내과학교실)
  • Published : 2010.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : This study was carried out to investigate the effects of Goihwa-san(GHS) against ulcerative colitis induced by DSS (dextran sulfate sodium). Method : The sample group was divided into three. The control group consisted of mice that were not inflammation-induced. The pathological group was composed of untreated colitis elicited mice. The experimental group was administered GHS after colitis elicitation. The effects on ulcerative colitis were evaluated by the morphological change of colonic mucosa, the anti-oxidant effect, HSP 70, NF-${\kappa}$B, COX-1, COX-2 and iNOS. Results : In terms of immunohistochemical changes, the distribution of COX-1 in mice treated with GHS increased noticeably more than that in the pathological group. The distributions of HSP70, NF-${\kappa}$B, COX-2, iNOS in mice treated with GHS more decreased than those in the pathological group. Regeneration of surface epithelial cell and goblet cell in mucosa was observed by optical microscope. The colonic lengths in GHS-treated mice were more elongated than those of DSS only treated mice. Conclusion : GHS is a candidate treatment for ulcerative colitis.

Keywords

References

  1. 김정룡. 소화기계 질환. 서울: 일조각; 2005, p. 277-315.
  2. 해리슨내과학편찬위원회. 해리슨내과학. 서울:도서출판MIP; 2006, p. 1937-50.
  3. 최은영. Indomethacan으로 유발된 생쥐의 대장점막 손상에 대한 赤小豆當歸散의 치료효과. 대한한방내과학회지. 2005;26(2):341-52.
  4. 최준혁, 임성우. 沙蔘麥門冬湯이 궤양성 대장염 유발흰쥐에 미치는 영향. 대한한의학회지. 2002; 23(4):169-85.
  5. 이주아, 공경환. DSS로 유발된 생쥐의 대장점막 손상에 대한 導滯湯의 효과. 대한한방내과학회지. 2008;29(3):752-64.
  6. 이성환, 최흥민, 임성우. DSS으로 유발된 생쥐의 대장염에 미치는 地楡湯의 효과. 대한한의학회지. 2007;28(1):187-97.
  7. 김승욱, 임성우. 導赤地楡湯이 궤양성 대장염 유발 흰쥐에 미치는 영향. 대한 한의학회지. 2002;23(3):200-10.
  8. Yu J, Venkata S, Lei Y, Anne BH, Xiangli C, Patricia A et al. American ginseng suppresses inflammation and DNA damage associated with mouse colitis. Carcinogenesis. 2008;29(12):2351-59. https://doi.org/10.1093/carcin/bgn211
  9. Chung HL, Yue GL, To KF, Su YL, Huang Y, Ko WH. Effect of Scutellariae Radix extract on experimental dextran-sulfate sodium-induced colitis in rats. World Gastroenterol. 2007;13(42):5605-11. https://doi.org/10.3748/wjg.v13.i42.5605
  10. 전국한의과대학 비계내과학교실. 脾系內科學. 서울: 군자출판사; 2008, p. 36-75.
  11. 朱震亨原著, 方廣編註. 丹溪心法附餘上卷. 서울: 대성문화사; 1993, p. 406.
  12. 許浚. 東醫寶鑑. 서울: 동의보감출판사; 2005, p. 138-40.
  13. 송인성. 한국인의 염증성 장질환. 대한내과학회지. 1999;57(4):661-74.
  14. Itzkowitz SH, Yio X. Inflammation and cancer IV. Colorectal cancer in Inflammatory bowel disease: the role of inflammation. Am J Physiol Gastrointest Liver Physiol. 2004;287(1):7-17. https://doi.org/10.1152/ajpgi.00079.2004
  15. Surh YJ, Chun KS, Cha HH, Han SS, Keum YS, Park KK, Lee SS. Molecular mechanisms underlying chemopreventive activities of anti-inflammatory phytochemicals: down-regulation of COX-2 and iNOS through suppression of NF-kappa B activation. Mutat Res. 2001;1:480-1.
  16. Fazio V. Current status of surgery for inflammatory bowel disease. Digestion. 1998;59(5):470-80. https://doi.org/10.1159/000007524
  17. 陳師文. 太平惠民和劑局方. 中國:施風出版社;中華民國64年, 券3, 第四葉.
  18. 강순수. 바른방제학. 서울: 대성문화사; 1996, p. 102-3.
  19. 康秉秀, 高雲彩, 金圭烈, 金先熙, 金仁洛, 金頀哲 등. 本草學. 서울: 영림사; 2000, p. 394, 615, 579.
  20. Baeuerle PA. $I{\kappa}b-NF-{\kappa}B$ structure: at the interface of inflammation control. Cell. 1998;95:729-31. https://doi.org/10.1016/S0092-8674(00)81694-3
  21. Anggard E. Nitric oxide: mediator, murderer, and medicine. Lancet. 1994;9:1199-206.
  22. Radi R, Beckman JS, Bush KM, Freeman BA. Peroxynitrite-induced membrane lipid peroxidation: the cytotoxic potential of superoxide and nitric oxide. Arch Biochem Biophys. 1991;288(2):481-7. https://doi.org/10.1016/0003-9861(91)90224-7
  23. Villa LM, Salas E, Darley-Usmar VM, Radomski MW, Moncada S. Peroxynitrite induces both vasodilatation and impaired vascular relaxation in the isolated perfused rat heart. Proc Natl Acad Sci USA. 1994;91(26):12383-7. https://doi.org/10.1073/pnas.91.26.12383
  24. Barnes PJ, Karin M. Nuclear factor-kappaB: a pivotal transcription factor in chronic inflammatory diseases. Engl J Med. 1997;336(15):1066-71. https://doi.org/10.1056/NEJM199704103361506
  25. Baeuerle PA, Baltimore D. $NF-{\kappa}B$ - Ten years after. Cell. 1996;87(1):13-20. https://doi.org/10.1016/S0092-8674(00)81318-5
  26. Groszmann RJ. Hyperdynamic state in chronic liver diseases. J Hepatol. 1993;17(2):38-40. https://doi.org/10.1016/S0168-8278(05)80454-4
  27. RothkOtter HJ, Pabst P, Bailey M. Lymphocyte migration in the intestinal mucosa : entry, transit and emigration of lymphoid cells and the influence of antigen. Vetrinary Immuno immunopath. 1999; 72:157-65. https://doi.org/10.1016/S0165-2427(99)00128-2
  28. Tsujii M, Kawano S, Tsuji S, Sawaoka H, Hori M, DuBois RN, Cyclooxygenase regulates angiogenesis induced by colon cancer cells. Cell 1998;93(5):705-16. https://doi.org/10.1016/S0092-8674(00)81433-6
  29. Kim HS, Berstad A. Experimental colitis in animal models. Scand J Gastroenterol. 1992;27(7):529-37. https://doi.org/10.3109/00365529209000116
  30. Bjorck S, Jennische E, Dahlstrom A. Influence of topical rectal application of drugs on dextran sulfate-induced colitis in rats. Ahlman Dig Dis Sci. 1997;42(4):824-32. https://doi.org/10.1023/A:1018880501437
  31. Gassler N, Rohr C, Schneider A, Kartenbeck J, Bach A, Obermuller N, Otto HF, Autschbach. Inflammatory bowel disease is associated with changes of enterocytic junctions. Am J Physiol Gastrointest Liver Physiol. 2001;281(1):216-28. https://doi.org/10.1152/ajpgi.2001.281.1.G216
  32. Kucharzik T, Walsh SV, Chen J, Parkos CA, Nusrat A. Neutrophil transmigration in inflammatory bowel disease is associated with differential expression of epithelial intercellular junction proteins. Am J Pathol. 2001;159(6):2001-9. https://doi.org/10.1016/S0002-9440(10)63051-9
  33. Gonzalez MF, Shiraishi K, Hisanaga K, Sagar SM, Mandabach M, Sharp FR. Heat shock proteins as markers of neuronal injury. Brain Res Mol Brain Res. 1989;6(1):93-100. https://doi.org/10.1016/0169-328X(89)90033-8
  34. Vass K., Beger ML., Nowak TS Jr, Welch WJ, Lassmann H. Induction of stress protein HSP70 in nerve cell after status epilepticus in the rat. Neurosci Lett. 1989;100(1-3):259-64. https://doi.org/10.1016/0304-3940(89)90695-2
  35. Bergstedt K, Hu BR., Weiloch T. Initiation of protein synthesis and heat shock protein-72 expression in the rat brain following sereve insulin induced hypoglycemia. Acta Neuropathol. 1993;86(2):145-53. https://doi.org/10.1007/BF00334881
  36. Choitsu S. Roles of COX-1 and COX-2 in gastrointestinal patho-physiology. J Gastroenterol. 1998;33:618-24. https://doi.org/10.1007/s005350050147