The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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The effect of Sagunja-tang on TNBS-induced Inflammatory Bowel Disease in Mouse

사군자탕(四君子湯)이 TNBS로 유발(誘發)된 생쥐의 염증성(炎症性) 장질환(腸疾患)에 미치는 영향(影響)

  • Hong, Sang-Sun (3rd Dept. of Internal Medicine, College of Oriental Medicine, Kyung-Hee University) ;
  • Ryu, Bong-Ha (3rd Dept. of Internal Medicine, College of Oriental Medicine, Kyung-Hee University) ;
  • Yoon, Seong-Woo (3rd Dept. of Internal Medicine, College of Oriental Medicine, Kyung-Hee University) ;
  • Kim, Jin-Sung (3rd Dept. of Internal Medicine, College of Oriental Medicine, Kyung-Hee University)
  • 홍상선 (경희대학교 한의과대학 비계내과학교실) ;
  • 류봉하 (경희대학교 한의과대학 비계내과학교실) ;
  • 윤성우 (경희대학교 한의과대학 비계내과학교실) ;
  • 김진성 (경희대학교 한의과대학 비계내과학교실)
  • Published : 2010.12.30
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Abstract

Objectives : The present study aimed to find out the effect of Sagunja-tang on the prevention and treatment of inflammatory bowel disease using mice with TNBS-induced inflammatory bowel disease. Methods : Mice with TNBS-induced inflammatory bowel disease were medicated with Sagunja-tang, and the weight changes, colon length, lipid peroxidation, and myeloperoxidase activity were observed. Levels of the inflammatory markers interleukin (IL)-$1{\beta}$ and cyclooxygenase-2 (COX-2), its transcription factor activation, phospho-NF-${\kappa}$B (pp65), in the colon by enzyme-linked immunosorbent assay and immunoblot analysis were also measured. Finally, the activation of fecal bacterial enzyme, ${\beta}$-glucuronidase and degradation activation of fecal glycosaminoglycan (GAG) and hyaluronic acid were observed. Results : We found that oral administration of Sagunja-tang inhibited TNBS-induced colon shortening and also inhibited myeloperoxidase activity in the colon of mice as well as IL-$1{\beta}$ and COX-2 expression. Sagunja-tang also inhibited TNBSinduced lipid peroxidation and pp65 activation in the colon of mice. In addition, Sagunja-tang inhibited ${\beta}$-glucuronidase activation and fecal hyaluronic acid degradation activation. Conclusions : It is supposed that Sagunja-tang has a potential therapeutic effect on inflammatory bowel disease through the inhibition of both NF-${\kappa}$B activation and lipid peroxidation, and the improvement of intestinal conditions.

Keywords

References

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