KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
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Antioxidative and Anti-diabetic Activity of C3GHi, Novel Black Rice Breed

Cyanidine-3-glucoside (C3G) 색소의 함유량이 증대된 쌀 신품종(C3GHi)의 항산화 및 항당뇨 활성

  • 김화영 ((주)이롬 생명과학연구원) ;
  • 김중학 ((주)이롬 생명과학연구원) ;
  • 이성애 ((주)이롬 생명과학연구원) ;
  • 류수노 (한국방송통신대학교 자연과학대학 농학과) ;
  • 한상준 (한국방송통신대학교 자연과학대학 농학과) ;
  • 홍성길 ((주)이롬 생명과학연구원)
  • Received : 2009.11.24
  • Published : 2010.03.31
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Abstract

C3GHi, a novel black rice variety developed by conventional breeding has more high contents of cyanidine-3-glucoside (C3G) and a more strong antioxidant than normal black rice. In this study, we investigate the antioxidative potential and anti-diabetic activity of C3GHi and extract of it for the purpose of development of functional materials or replacement meal for diabetic patients. Using in vitro antioxidative activity assay system such as DPPH radical quenching assay, superoxide anion radical scavenging assay, inhibition of lipid peroxidation and DNA breakage assay, C3GHi extract was observed to have more high antioxidative activity than normal black rice breed. The glycemic index of a freeze dried powder of C3GHi brown rice showed $43.7\pm18.8$, which belonged to low GI food (GI of 55 or less). Using in vivo diabetic model such as db/db mice model and streptozotocininduced diabetic model, C3GHi rice extract decrease blood glucose level and inhibit oxidative stress in blood. From the results, we think that the C3GHi varieties have a potential for the functional materials or ingredient of meal replacement for diabetic patients.

C3GHi 쌀 품종은 흑진주벼와 수원425호를 교배조합으로 하여 색소 성분인 cyanidine-3-glucoside(C3G)의 함량을 비약적으로 증대시킨 품종이다. C3GHi 벼품종을 활용하여 기능성 제품 및 소재로의 개발 가능성을 타진하기 위하여 본 연구에서는 C3GHi 벼품종의 항산화 활성 및 항당뇨 기능의 검증을 수행하였다. 1. C3GHi 벼품종의 항산화 활성을 비교 분석한 결과에서 C3GHi는 일반 벼 및 흑진주벼보다 매우 우수한 항산화 활성을 가진 것으로 나타났으며, 대표적 항산화 소재인 glutathione과 유사한 활성을 나타내었다. 2. C3GHi 벼품종의 혈당지수를 인체 시험을 통해서 산출한 결과에서도 일반 현미나 백미에 비해서 15%정도 낮은 혈당지수를 나타내 당뇨 환자들의 식사 대용식의 소재로서 활용 가능성이 높을 것으로 사료된다. 3. db/db mice 모델 및 STZ를 통해 당뇨병을 유발한 동물 모델에서 C3GHi 쌀추출물 및 C3GHi쌀 현미의 동결건조 분말이 혈당 상승을 막고, 산화적 손상을 억제하는 것으로 나타났다. 4. 이상의 결과에서 C3GHi쌀은 낮은 혈당지수 및 항산화 활성, 항당뇨 활성 등을 보유하고 있어 이후 당뇨 환자용 기능성 식사 대용식 및 기능성 식품 원료로서의 개발 가능성이 높다고 판단된다.

Keywords

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