Effect of the Long-term Application of Organic Matters on Microbial Diversity in Upland Soils

유기물 장기 연용이 밭토양 미생물의 다양성에 미치는 영향

  • Suh, Jang-Sun (National Academy of Agricultural Science, R.D.A.) ;
  • Kwon, Jang-Sik (National Academy of Agricultural Science, R.D.A.) ;
  • Noh, Hyung-Jun (National Institute of Horticultural and Herbal Science, R.D.A.)
  • 서장선 (농촌진흥청 국립농업과학원) ;
  • 권장식 (농촌진흥청 국립농업과학원) ;
  • 노형준 (농촌진흥청 국립원예특작과학원)
  • Received : 2010.11.01
  • Accepted : 2010.12.20
  • Published : 2010.12.31

Abstract

To investigate the effect of long term application of organic matter in upland soils, plots for treatments of NPK, NPK+pig manure compost, rape seed cake, rice straw compost, and green manure were set up. Populations of Bacillus and Gram negative bacteria were high in the plot treated with green manure application, but microbial biomass was increased with chemical fertilizer or pig manure compost in upland soils. Activities of phosphomonoesterase and dehydrogenase were high with organic matter application comparing to control. Cluster patterns analysed using phospholipid fatty acid of plots treated with rice straw and or pig manure compost were clearly different comparing with other treatments. Dominant bacteria in upland soils were Bacillus flexus, B. subtilis and B. megaterium. And the strains isolated from upland soils had amylase, protease and lipase activities.

바실러스와 그람음성균은 녹비시용구에서 높았지만, 미생물량은 비료+돈분퇴비 시용구에서 높았다. 인산효소와 유기물분해효소는 무처리구에 비해 유기물 시용구에서 모두 높은 값을 보였다. 영년 밭토양의 PLFA에 의한 군집분석은 볏짚퇴비 및 돈분퇴비시용구가 다른 처리와 상이한 특성을 보였다. 영년 밭 토양의 우점균은 Bacillus flexus, Bacillus subtilis 및 Bacillus megaterium 등이었으며, 토양에서 분리된 세균은 대부분 amylase, protease 및 lipase의 활성을 가지고 있었다.

Keywords

References

  1. Allison, V.J, L.M. Condron, D.A. Peltzer, S.J. Richardson, and B.L. Turner. 2007. Changes in enzyme activities and soil microbial community composition along carbon and nutrient gradients at the Franz Josef chronosequence, New Zealand. Soil Biol. Biochem. 39:1770-1781. https://doi.org/10.1016/j.soilbio.2007.02.006
  2. Amoozegar, M.A., F. Malekzadeh, and K.A. Malik. 2003. Production of amylase by newly isolated moderate halophile, Halobacillus sp. strain MA-2. J. Microbiol. methods. 52:353-359. https://doi.org/10.1016/S0167-7012(02)00191-4
  3. Baath, E., and T.H. Anderson. 2003. Comparison of soil fungal/bacterial ratios in a pH gradient using physiological and PLFA-based techniques. Soil Biol. Biochem. 35: 955-963. https://doi.org/10.1016/S0038-0717(03)00154-8
  4. Crecchio, C., M. Curci, R. Mininni, P. Ricciuti, and P. Ruggiero. 2001. Short term effects of municipal solid waste compost amendments on soil carbon and nitrogen content, some enzyme activities and genetic diversity. Biol. Fertil. Soils. 34:311-318. https://doi.org/10.1007/s003740100413
  5. Criquet, S. and A. Braud. 2008. Effects of organic and mineral amendments on available P and phosphatase activities in a degraded Mediterranean soil under short-term incubation experiment. Soil Tillage Res. 98:164-174. https://doi.org/10.1016/j.still.2007.11.001
  6. Degens, B.P., L.A. Schipper, G.P. Sparling, and M. Vojvodic- Vukovich. 2000. Decreases in organic C reserves in soils can reduce the catabolic diversity of soil microbial communities. Soil Biol. Biochem. 32:189-196. https://doi.org/10.1016/S0038-0717(99)00141-8
  7. Dhillion, S.S. 1997. Fallow age influences microbial functional abilities, soil properties and plant functional groups. In: Insam, H., Rangger, A. (Eds.), Microbial Communities, Springer, Berlin, pp. 140-148.
  8. Donnison, L.M., G.S. Griffith, and R.D. Bardgett. 2000. Determinants of fungal growth and activity in botanically diverse hay meadows: effects of litter type and fertilizer additions. Soil Biol. Biochem. 32:289-294. https://doi.org/10.1016/S0038-0717(99)00160-1
  9. Geisseler, D., and W.R. Horwath. 2008. Regulation of extracellular protease activity in soil in response to different sources and concentrations of nitrogen and carbon. Soil Biol. Biochem. 40:3040-3048. https://doi.org/10.1016/j.soilbio.2008.09.001
  10. Harrison, U.J. and H.D. Shaw. 2001. Effects of soil pH and nitrogen fertility on the population dynamics of Thielaviopsis basicola. Plant Soil. 228:147-155. https://doi.org/10.1023/A:1004845715885
  11. Hopkins, D.W. and R.S. Shiel. 1996. Size and activity of soil microbial communities in long-term experimental grassland plots treated with manure and inorganic fertilizers. Biol. Fertil. Soils. 22:66-70. https://doi.org/10.1007/BF00384434
  12. Jensen, L.E. and O. Nybroe. 1999. Nitrogen availability to Pseudomonas fluorescens DF57 is limited during decomposition of barley straw in bulk soil and in the barley rhizosphere. Applied Environmental Microbiology. 65:4320-4328.
  13. Kandeler, E., J. Luxhoi, D. Tscherko, and J. Magid. 1999a. Xylanase, invertase and protease at the soil-litter interface of a loamy sand. Soil Biol. Biochem. 31:1171-1179. https://doi.org/10.1016/S0038-0717(99)00035-8
  14. Kandeler, E., M. Stemmer, and E.M. Klimanek. 1999b. Response of soil microbial biomass, urease and xylanase within particle size fractions to long-term soil management. Soil Biol. Biochem. 31:261-273. https://doi.org/10.1016/S0038-0717(98)00115-1
  15. Madejon, E., P. Burgos, R. Lopez, and F. Cabrera. 2001. Soil enzymatic response to addition of heavy metals with organic residues. BBiol. Fertil. Soils. 34:144-150. https://doi.org/10.1007/s003740100379
  16. Mavingui, P. and T. Heulin. 1994. In vitro chitinase and antifungal activity of a soil, rhizosphere and rhizoplane population of Bacillus polymyxa. Soil Biol. Biochem. 26:801-803. https://doi.org/10.1016/0038-0717(94)90277-1
  17. Marschner, B. and A. Noble. 2000. Chemical and biological processes leading to the neutralisation of soil acidity after incubation with different litter materials. Soil Biol. Biochem. 32:805-813. https://doi.org/10.1016/S0038-0717(99)00209-6
  18. Metting, F.B. 1993. Structure and physiological ecology of soil microbial communities. In: Metting, F.B., (Ed.), Soil Microbial Ecology-Application in Agricultural and Environmental Management, Marcel Dekker, New York, pp. 3-24.
  19. Parham, J.A., S.P. Deng, W.R. Raun, and G.V. Johnson. 2002. Long-term cattle manure application in soil. Part I: effect on soil phosphorus levels and activities of enzymes involved in phosphorus transformations in soil. Biol. Fertil. Soils. 35:328-337. https://doi.org/10.1007/s00374-002-0476-2
  20. Parham, J.A., S.P. Deng, and W.R. Raun. 2003. Long-term cattle manure application in soil. Part II: effect on soil microbial populations and community structure. Biol. Fertil. Soils. 38:209-215. https://doi.org/10.1007/s00374-003-0657-7
  21. Pascual, J.A., C. Garcia, T. Hernandez, J.L. Moreno, and M. Ros. 2000. Soil microbial activity as a biomarker of degradation and remediation processes. Soil Biol. Biochem. 32:1877-1877. https://doi.org/10.1016/S0038-0717(00)00161-9
  22. Peacock, A.D., M.D. Mullen, D.B. Ringelberg, D.D. Tyler, D.B. Hedrick, P.M. Gale, and D.C. White. 2001. Soil microbial community responses to dairy manure or ammonium nitrate applications. Soil Biol. Biochem. 33:1011-1019. https://doi.org/10.1016/S0038-0717(01)00004-9
  23. Plaza, C., D. Hernandez, J.C. Garcia-Gil, and A. Polo. 2004. Microbial activity in pig slurry-amended soils under semiarid conditions. Soil Biol. Biochem. 36:1577-1585. https://doi.org/10.1016/j.soilbio.2004.07.017
  24. Sarniguet, A., P. Lucas, and M. Lucas. 1992. Relationships between take-all, soil conduciveness to the disease, populations of fluorescent pseudomonads and nitrogen fertilizers. Plant Soil. 145:17-27. https://doi.org/10.1007/BF00009537
  25. Sarathchandra, S.U., A. Ghani, G.W. Yeates, G. Burch,and N.R. Cox. 2001. Effect of nitrogen and phosphate fertilizers on microbial and nematode diversity in pasture soils. Soil Biol. Biochem. 33:953-964. https://doi.org/10.1016/S0038-0717(00)00245-5
  26. Starnes, D.L., P. Padmanabhan, and S.V. Sahi. 2008. Effect of P sources on growth, P accumulation and activities of phytase and acid phosphatases in two cultivars of annual ryegrass (Lolium multiflorum L.). Plant Physiol. Biochem. 46:580-589. https://doi.org/10.1016/j.plaphy.2007.06.002
  27. Suh, J.S., H.J. Noh, and J.S. Kwon. 2008. Effects of amendments on the phosphate-solubilizing bacteria in rice paddy soils. J. Korean Soc. Soil Sci. Fert. 41:342-347.
  28. Suh, J.S., H.J. Noh, and J.S. Kwon. 2009. Impact of amendments on microbial biomass, enzyme activity and bacterial diversity of soils in long-term rice field experiment. Korean J. Soil Sci. Fert. 42:257-265.
  29. Sukul, P. 2006. Enzymatic activities and microbial biomass in soil as influenced by metalaxyl residues. Soil Biol. Biochem. 38:320-326. https://doi.org/10.1016/j.soilbio.2005.05.009
  30. Wang, S.L, T.Y. Lin, Y.H. Yen, H.F. Liao, and Y.J. Chen. 2006. Bioconversion of shellfish chitin wastes for the production of Bacillus subtilis W-118 chitinase. Carbohydrate research. 341:2507-2515. https://doi.org/10.1016/j.carres.2006.06.027
  31. 농촌진흥청. 1988. 토양화학분석법. 삼미인쇄사.
  32. 토양미생물연구회. 1992. 토양미생물실험법. 양현당.