The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Comparative Study of Anti-oxidant and Anti-inflammatory Activities between Curcumae longae Radix and Curcumae longae Rhizoma

울금과 강황의 항산화 및 항염증 활성 비교연구

  • Oh, Hye-In (Dept. of Life and Nanopharmaceutical Science, College of Pharmacy, Kyung Hee University) ;
  • Park, Han-Byeol (Dept. of Life and Nanopharmaceutical Science, College of Pharmacy, Kyung Hee University) ;
  • Ju, Mi-Sun (Dept. of Oriental Pharmaceutical Science, College of Pharmacy, Kyung Hee University) ;
  • Jung, Sun-Yong (Dept. of Neuropsychiatry, College of Oriental Medicine, Kyung Hee University) ;
  • Oh, Myung-Sook (Dept. of Life and Nanopharmaceutical Science, College of Pharmacy, Kyung Hee University)
  • 오혜인 (경희대학교 약학대학 나노의약생명과학과) ;
  • 박한별 (경희대학교 약학대학 나노의약생명과학과) ;
  • 주미선 (경희대학교 약학대학 한약학과) ;
  • 정선용 (경희대학교 한의과대학 신경정신과학교실) ;
  • 오명숙 (경희대학교 약학대학 나노의약생명과학과)
  • Received : 2010.02.25
  • Accepted : 2010.03.22
  • Published : 2010.03.30
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Abstract

Objectives : In this study, we compared the anti-oxidant and anti-inflammatory activities of Curcumae longae Radix (CLRa) and Curcumae longae Rhizoma (CLRh). Methods : We performed 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and 2,2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) cation scavenging assays, and determined total polyphenolic content to examine the anti-oxidant effects of CLRa and CLRh. We also evaluated the anti-oxidant effects of CLRa and CLRh against hydrogen peroxide ($H_2O_2$)-induced toxicity in PC12 cells using thiazolyl blue tetrazolium bromide (MTT) and reactive oxygen species (ROS) assays. Next, to compare the anti-inflammatory effects of CLRa and CLRh against lipopolysaccharide (LPS)-induced inflammation in microglia BV2 cells, we measured nitric oxide (NO) assay and inducible nitrite synthase (iNOS) using Western blotting analysis. Results : CLRa showed higher activity in DPPH and ABTS assays and lower total polyphenolic contents compared with CLRh. In PC12 cells, CLRa and CLRh showed no difference in H2O2-induced cell toxicity and ROS overproduction. In BV2 cells, CLRa showed higher effect than CLRh in NO and iNOS production induced by LPS. Conclusions : These results demonstrate that CLRa has higher radical scavenging activities and anti-inflammatory effect in BV2 cells comparing CLRh. However, CLRa and CLRh have no effect and no difference in $H_2O_2$-induced toxicity.

Keywords

References

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