Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Antioxidative Effect and Melanogenesis of Nelumbo nucifera Stamen Extract on Cultured Human Skin Melanoma Cells Injured by Hydrogen Peroxide

연꽃수술추출물이 과산화수소로 손상된 배양 인체피부흑색종세포에 대한 항산화효과 및 멜라닌화에 미치는 영향

  • Kim, Myoung-Seoup (Department of Environmental Health Science, Wonkwang University) ;
  • Park, Yun-Jum (Division of Horticulture and Animal-Plant Science, Wonkwang University) ;
  • Sohn, Young-Woo (Sanbon Medical Center, Wonkwang University)
  • Received : 2009.12.19
  • Accepted : 2010.03.04
  • Published : 2010.04.30
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Abstract

To examine the antioxidative effect and melanogenesis of Nelumbo nucifera stamen (NNS) extract on hydrogen peroxide $H_2O_2$ induced cytotoxicity in cultured human skin melanoma cells (SK-MEL-3), cell adhesion activity (CAA), tyrosinase inhibitory activity and total amount of melanin synthesis were measured by colorimetric assay. In this study, $H_2O_2$ significantly decreased CAA, and $CAA_{50}$ value of $H_2O_2$ was determined at 30 uM. In the antioxidative effect, NNS extract increased cell adhesion activity which was decreased by $H_2O_2$ induced cytotoxicity, and also, tyrosinase activity and total amount of melanin were decreased by NNS extract. These results suggested that $H_2O_2$ was highly toxic on cultured human skin melanoma cells and NNS extract showed the antioxidative and inhibitory effect of melanogenesis by the increased CAA, and the decresed tyrosinase activity and total amount of melanin synthesis.

활성산소의 하나인 과산화수소(hydrogen peroxide, $H_2O_2$)에 대한 세포독성과 이에 대한 연꽃수술(Nelumbo nucifera stamen, NNS)추출물의 항산화효과 및 멜라닌화에 미치는 영향을 알아보기 위하여 세포부착율 (cell adhesion activity, CAA)을 비롯한 티로시나제활성저해능 및 총멜라닌합성량을 분석하였다. 본 실험 결과 hydrogen peroxide($H_2O_2$)는 배양 인체피부흑색종세포 (SK-MEL-3)의 CAA를 유의하게 감소시킴으로서 세포독성효과를 나타냈다. 이에 대하여 NNS추출물은 $H_2O_2$의 산화적 손상에 의하여 감소된 CAA를 유의하게 증가시킴으로서 항산화효과를 나타냈다. 한편, $H_2O_2$에 대한 NNS추출물의 멜라닌화에 대한 영향에 있어서 NNS추출물은 $H_2O_2$에 의하여 활성화된 티로시나제활성과 총멜라닌합성량을 감소시킴으로서 멜라닌화에 대하여 억제효과를 나타냈다. 이상의 결과로 부터 $H_2O_2$는 배양 인체피부흑색종세포에 고독성을 나타냈으며 NNS와 같은 식물추출물은 $H_2O_2$와 같은 활성산소에 의한 산화적 손상 및 멜라닌화를 방어하는데 효과적인 것으로 나타났다.

Keywords

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