Analytical Science and Technology (분석과학)

The Korean Society of Analytical Sciences (한국분석과학회)
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Analysis and risk assessment of polycyclic aromatic hydrocarbons (PAHs) in seafood from oil contaminated bay

유류 오염지역의 수산물 중 다환방향족탄화수소류 (PAHs) 분석 및 위해평가

  • Jeong, Ji-Yoon (Risk Analysis & Research Division, National Institute of Food and Drug Safety Evaluation, Korea Food and Drug Administration) ;
  • Choi, Chan-Woong (Risk Analysis & Research Division, National Institute of Food and Drug Safety Evaluation, Korea Food and Drug Administration) ;
  • Ryeom, Tai-Kyung (Risk Analysis & Research Division, National Institute of Food and Drug Safety Evaluation, Korea Food and Drug Administration) ;
  • Cho, Kyoung-Hee (Risk Analysis & Research Division, National Institute of Food and Drug Safety Evaluation, Korea Food and Drug Administration) ;
  • Park, Se-Ryung (Risk Analysis & Research Division, National Institute of Food and Drug Safety Evaluation, Korea Food and Drug Administration) ;
  • Shin, Ho-Sang (Dept. of Environmental Education, Kongju National University) ;
  • Lee, Kwang-Ho (Risk Analysis & Research Division, National Institute of Food and Drug Safety Evaluation, Korea Food and Drug Administration) ;
  • Lee, Hyo-Min (Risk Analysis & Research Division, National Institute of Food and Drug Safety Evaluation, Korea Food and Drug Administration)
  • 정지윤 (식품의약품안전평가원 위해분석연구과) ;
  • 최찬웅 (식품의약품안전평가원 위해분석연구과) ;
  • 염태경 (식품의약품안전평가원 위해분석연구과) ;
  • 조경희 (식품의약품안전평가원 위해분석연구과) ;
  • 박세령 (식품의약품안전평가원 위해분석연구과) ;
  • 신호상 (공주대학교 환경교육과) ;
  • 이광호 (식품의약품안전평가원 위해분석연구과) ;
  • 이효민 (식품의약품안전평가원 위해분석연구과)
  • Received : 2009.11.25
  • Accepted : 2010.02.23
  • Published : 2010.04.25
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Abstract

To assess health risk for the intake among residents after the Hebei Spirit oil spill, 16 Polycyclic Aromatic Hydrocarbons (PAHs) in seafood samples from oil contaminated bay were determined by Gas Chromatography-Mass Spectrometry (GC-MSD) and samples were personally collected and purchased by residents. Samples were hydrolyzed with KOH and extracted with methylene chloride. The extracted solution were cleaned up using silica/florisil column and 16 PAHs were eluted by methylene chloride : n-hexane (1:9) mixture and determined by GC-MSD in Selected Ion Monitoring (SIM) mode. The mean recoveries for 16 PAHs ranged from 79% to 85%. The 16 PAHs levels in 126 samples ranged from 0.17 to $6.04\;{\mu}g$/kg and the TEQBaP (Toxic EQuivalents) levels in 126 samples were calculated using benzo(a)pyrene toxic equivalency factor for individual 16 PAHs and ranged from 0 to $0.91\;{\mu}gTEQ$/kg. The average Benzo(a)pyrene dietary exposure of residents was $5.5{\times}10^{-8}\;mg/kg$ bw/day and the average PAHs chronic dietary exposure was $1.3{\times}10^{-5}\;mg$ TEQ/kg bw/day. The margin of exposure (MOE) and the excess cancer risk and were $1.8{\times}10^6$ and $9.8{\times}10^{-8}$, respectively. Therefore, the assessment result was considered as low concern for health risk.

허베이 스피리트호 유류유출 사고 후 지역주민들의 수산물 섭취로 인한 건강 위해도를 평가하기 위하여, 가스크로마토그래피-질량분석기(GC-MSD)를 이용하여 유류유출 지역 주민들이 직접 채취 및 구입한 수산물 중 다환방향족탄화수소류(PAHs) 16종을 분석하였다. 시료는 KOH로 가수분해하여 Methylene chloride로 추출하였다. 추출액 중의 16개의 PAHs는 실리카/플로리실 정제컬럼을 이용하여 Methylene chloride : n-헥산(1:9) 혼합용액으로 용출하였고 GC-MSD의 SIM을 이용하여 분석하였다. 16 개의 PAHs의 평균 회수율은 79~85%이었으며, 126개의 시료에서 $0.17\sim6.04\;{\mu}g/kg$ 수준으로 검출되었고, Benzo(a)pyrene의 독성등가계수(TEF)를 적용한 Benzo(a)pyrene 독성등가량($TEQ_{BaP}$)은 $0\sim0.91\;{\mu}gTEQ$/kg 수준이었다. 또한 유류유출 지역 주민들의 벤조피렌의 일일 평균 노출량은 $5.5{\times}10^{-8}\;mg/kg$ bw/day 이었고, PAHs 일일 평균 만성노출량은 $1.3{\times}10^{-5}\;mgTEQ$/kg bw/day이었다. 그리고 안전마진(MOE)과 초과발암위해도는 각각 $1.8{\times}10^6$, $9.8{\times}10^{-8}$으로 사람에게 위해영향발생 우려가 낮은 수준이었다.

Keywords

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