Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Identification of the Oligotrophic Bacteria Strain 7F Biocontrolling Phytophthora Blight Disease of Red-pepper

고추 역병 방제를 위한 저영양 길항세균 7F 균주의 동정

  • Kim, Dong-Gwan (Research Center for Drugs, Konkuk University) ;
  • Yeo, Yun-Soo (National Agrobiodiversity Center, National Academy of Agricultural Science, RDA) ;
  • Kwon, Soon-Wo (National Agrobiodiversity Center, National Academy of Agricultural Science, RDA) ;
  • Jang, Kil-Su (Youngyang Pepper Experiment Station, Gyeongbuk Provincial RDA Youngyang) ;
  • Lee, Chang-Muk (Dept. of Functional Materials Development, National Academy of Agricultural Science, RDA) ;
  • Lee, Mi-Hye (Dept. of Technical Support, Yeoju-gun Agricultural Technology Center) ;
  • Kim, Soo-Jin (National Agrobiodiversity Center, National Academy of Agricultural Science, RDA) ;
  • Koo, Bon-Sung (Dept. of Functional Materials Development, National Academy of Agricultural Science, RDA) ;
  • Yoon, Sang-Hong (Dept. of Functional Materials Development, National Academy of Agricultural Science, RDA)
  • 김동관 (건국대학교 의약연구센터) ;
  • 여윤수 (농촌진흥청 국립유전자원센터) ;
  • 권순우 (농촌진흥청 국립유전자원센터) ;
  • 장길수 (경상북도 영양고추시험장) ;
  • 이창묵 (농촌진흥청 국립농업과학원 기능성물질 개발과) ;
  • 이미혜 (농촌진흥청 여주 농업기술센터) ;
  • 김수진 (농촌진흥청 국립유전자원센터) ;
  • 구본성 (농촌진흥청 국립농업과학원 기능성물질 개발과) ;
  • 윤상홍 (농촌진흥청 국립농업과학원 기능성물질 개발과)
  • Received : 2010.01.15
  • Published : 2010.04.01
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Abstract

A total of 10,753 oligotrophic bacteria were isolated from the cultivated soils of red-pepper infected by Phytophthora blight disease in various regions of Korea (Chungju, Anmyon, Taean, Andong, Eumsung and Goesan). Seven bacteria isolates among these collected resources were selected by the first screening of in vitro antagonistic assay against major several plant pathogenic fungi including Phytophthora capsici. Finally, strain 7F was selected by pot assay for a possible biological control agent against Phytophthora blight disease of pepper seedling in the greenhouse. Strain 7F was identified as Bacillus subtilis on the basis of its 16S rDNA sequence analysis and as standardized biochemical characteristics assay kits such as API20 NE. In the experiment of P. capsici zoospore infected red-pepper on the pot test, infection rate of red-pepper with nonetreatment to Phytophthora blight disease was 87%, while the rate was only 6% in the pot treated with strain 7F. This result indicated that the Bacillus subtilis strain 7F will be useful as a potential biocontrol agent for Phytophthora blight disease of red-pepper.

고추 역병이 발생한 충주, 안면, 태안, 안동, 음성 그리고 괴산지역의 고추밭 토양에서 전체 10,753균주의 저영양 미생불을 분리하였다. 이들 중 Phytophthora capsid를 포함하여 식불체의 주요 병원성 곰팡이에 대해 길항작용을 보이는 균주들을 일차적으로 선발하였다. 최종적으로는 온실 고추유묘 검정을 통해 고추 역병에 대해 탁월한 방제가를 보이는 7F 균추가 선발되었다. 이 균주는 16SrDNA염기서열 분석과 API20 NE 키트를 통한 생리, 생화학적 분석을 통하여 Bacillus subtilis로 동정 되었다. 고추 역병균 포자를 처리한 고추 유묘 화분 검정에서, 처리25일 후 대조구는 고추 역병 이병 비율이 87%에 이르는 반면에 7F 균주를 처리한 처리구에서는 단지 6%의 이병율을 보였다.

Keywords

References

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