Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Galactinol is Involved in Induced Systemic Resistance against Bacterial Infection and Environmental Stresses

  • Cho, Song-Mi (Department of Floriculture, Chunnam Techno College) ;
  • Kim, Su-Hyun (Department of Plant Biotechnology, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Kim, Young-Cheol (Department of Plant Biotechnology, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Yang, Kwang-Yeol (Department of Plant Biotechnology, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Kim, Kwang-Sang (Jangheunggun Mushroom Research Institute) ;
  • Choi, Yong-Soo (Department of Floriculture, Chunnam Techno College) ;
  • Cho, Baik-Ho (Department of Plant Biotechnology, College of Agriculture and Life Sciences, Chonnam National University)
  • Received : 2009.11.16
  • Accepted : 2010.03.02
  • Published : 2010.06.30
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Abstract

We previously demonstrated that root colonization of the rhizobacterium, Pseudomonas chlororaphis O6, induced expression of a galactinol synthase gene (CsGolS1), and resulting galactinol conferred induced systemic resistance (ISR) against fungal and bacterial pathogens in cucumber leaves. To examine the role of galactinol on ISR, drought or high salt stress, we obtained T-DNA insertion Arabidopsis mutants at the AtGolS1 gene, an ortholog of the CsGolS1 gene. The T-DNA insertion mutant compromised resistance induced by the O6 colonization against Erwinia carotovora. Pharmaceutical application of 0.5 - 5 mM galactinol on roots was sufficient to elicit ISR in wild-type Arabidopsis against infection with E. carotovora. The involvement of jasmonic acid (JA) signaling on the ISR was validated to detect increased expression of the indicator gene PDF1.2. The T-DNA insertion mutant also compromised tolerance by increasing galactinol content in the O6-colonized plant against drought or high salt stresses. Taken together, our results indicate that primed expression of the galactinol synthase gene AtGolS1in the O6-colonized plants can play a critical role in the ISR against infection with E. carotovora, and in the tolerance to drought or high salt stresses.

Keywords

References

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