Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
권호 표지

Genetic Relationship Analysis of genus Nelumbo Accessions Based on Inter-Simple Sequence Repeats (ISSR)

ISSR 표지에 의한 연속 (Nelumbo)의 유연관계 분석

  • Ryu, Jai-Hyunk (Department of Bioresources Science, Sunchon National University) ;
  • Choi, Gab-Lim (Business Incubation Center, Mokwon University) ;
  • Lyu, Jae-Il (Division of Biosystems Reserch, KRIBB) ;
  • Lee, Sheong-Chun (Department of Bioresources Science, Sunchon National University) ;
  • Chun, Jong-Un (Department of Bioresources Science, Sunchon National University) ;
  • Shin, Dong-Young (Department of Bioresources Science, Sunchon National University) ;
  • Bae, Chang-Hyu (Department of Bioresources Science, Sunchon National University)
  • 류재혁 (순천대학교 바이오자원학부) ;
  • 최갑림 (목원대학교 창업보육센터) ;
  • 류재일 (한국생명공학연구원) ;
  • 이성춘 (순천대학교 바이오자원학부) ;
  • 천종은 (순천대학교 바이오자원학부) ;
  • 신동영 (순천대학교 바이오자원학부) ;
  • 배창휴 (순천대학교 바이오자원학부)
  • Received : 2010.02.09
  • Accepted : 2010.04.05
  • Published : 2010.04.30
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Abstract

The polymorphism and the genetic relationships among 32 genetic resources of genus Nelumbo from Korea, Japan, China, USA, India, Thailand and Gabong were thoroughly investigated and extensively examined using ISSR markers. Out of 103 loci detected overall, 94 were identified to be polymorphic with a rate of 91.2%. The genetic similarity matrix revealed a wide range of variability among the 32 accessions, spanning from 0.227 to 0.833. The study findings indicate that the Nelumbo accessions have a high genetic diversity, and accordingly carry a germplasm qualifying as good genetic resources for cross breeding. According to the clustering analysis, different subspecies, N. nucifera and N. lutea, were divided into independent groups and all of the N. nucifera accessions could be classified into five categories. Compared to RAPD analysis, ISSR method showed a clearer picture of polymorphism among the accessions and exhibited a definite distinction even among the subspecies. In this respect, ISSR analysis is considered to be more effective in differentiating the accessions and subspecies of the genus Nelumbo than RAPD test.

Keywords

References

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