Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Effects of Dietary Supplementation of Fermented Garlic Powder on Immune Responses, Blood Components, and Disease Resistance against Principal Fish Disease of Juvenile Olive Flounder, Paralichthys olivaceus in Low Temperature Season

저수온기 넙치 치어에 있어서 발효마늘분말의 사료 내 첨가가 면역반응, 혈액성분 및 주요 어병세균에 대한 질병저항성에 미치는 영향

  • Kim, Sung-Sam (Faculty of Marine Biomedical Science, Jeju National University) ;
  • Song, Jin-Woo (Faculty of Marine Biomedical Science, Jeju National University) ;
  • Lim, Se-Jin (Faculty of Marine Biomedical Science, Jeju National University) ;
  • Jeong, Joon-Bum (Faculty of Marine Biomedical Science, Jeju National University) ;
  • Jeon, You-Jin (Faculty of Marine Biomedical Science, Jeju National University) ;
  • Yeo, In-Kyu (Faculty of Marine Biomedical Science, Jeju National University) ;
  • Lee, Kyeong-Jun (Faculty of Marine Biomedical Science, Jeju National University)
  • 김성삼 (제주대학교 해양의생명과학부) ;
  • 송진우 (제주대학교 해양의생명과학부) ;
  • 임세진 (제주대학교 해양의생명과학부) ;
  • 정준범 (제주대학교 해양의생명과학부) ;
  • 전유진 (제주대학교 해양의생명과학부) ;
  • 여인규 (제주대학교 해양의생명과학부) ;
  • 이경준 (제주대학교 해양의생명과학부)
  • Received : 2010.04.20
  • Accepted : 2010.07.19
  • Published : 2010.08.31
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Abstract

We report non-specific immune responses and disease resistance against Vibrio anguillarum, Streptococcus iniae and Edwardsiella tarda by dietary supplementation of fermented garlic powder (FGP) in olive flounder for the first time. Four isonitrogenous (45% crude protein) and isocaloric (17.1 MJ/kg) diets were formulated to have 0%, 0.5%, 1% and 2% of the FGP (G-0, G-0.5, G-1 and G-2). The experimental diets were fed to juvenile olive flounder averaging 23.4 g in triplicate groups (90 fish/group) in a flow-through system. After a five-week feeding trial, healthy fish with similar sizes from each tank were selected and injected with 1 ml of three bacteria each to evaluate disease resistance of the fish. During the 5-week feeding trial, the weight gain, specific growth rate, feed conversion ratio, protein efficiency ratio, and survival of the fish were not significantly affected by the experimental diets. However, feed intake was significantly lower (P<0.05) in the fish fed the G-2 diet compared with the control group. Hemoglobin, myeloperoxidase activity, cholesterol and HDL-cholesterol were not different between the dietary groups. However, hematocrit, nitroblue tetrazolium (NBT) activity, and lysozyme activity were increased (P<0.05) with an increment of dietary FGP. Plasma triglyceride of the fish fed the G-0.5 diet was significantly lower than that of fish fed the control diet. The cumulative mortality was lower in the fish fed diets containing FGP compared with the control group in the challenge test except for the bacteria Edwardsiella tarda. The results in this study indicate that dietary supplementation of FGP can enhance the non-specific immune responses and disease resistance of olive flounder against V. anguillarum and S. iniae.

우리 연구팀은 넙치를 대상으로 사료 내 발효마늘분말(fermented garlic powder, FGP)의 첨가에 의한 비특이적 면역반응과 어병세균(V. anguillarum, S. iniae, E. tarda)에 대한 질병 저항성에 대해 처음으로 보고한다. 동일한 단백질 함량(45%)과 칼로리(17.1 MJ/kg)를 갖은 4개의 실험사료는 발효마늘분말이 0%, 0.5%, 1% 및 2% (G-0, G-0.5, G-1 및 G-2)가 포함되도록 조성되었다. 실험은 3반복구로 수조 당 30마리의 어류를 대상으로 유수식 시스템에서 수행되었다. 5주간의 성장실험 종료 후, 각 수조에서 건강한 어류를 선별하여 어류의 항병력을 평가하기 위해 3가지 박테리아 부유액(1 ml)을 복강에 주사하였다. 5주간의 성장실험기간 동안에 성장률, 일간성장률, 사료전환효율, 단백질전환효율 및 생존율에서는 실험사료에 의한 유의적인 차이가 없었다. 그러나 사료섭취율은 발효마늘분말이 2% 첨가된 그룹에서 대조구와 비교하여 유의적으로 낮았다. 헤모글로빈, 헤마토크릿, 대식세포 활성 및 라이소자임 활성에서는 사료 내 발효마늘분말 함량이 증가함에 따라 유의적으로 증가하였다. 발효마늘분말 0.5% 첨가구의 혈장 중성지질은 대조구보다 유의적으로 낮았다. 누적사망률은 E. tarda의 경우를 제외한 모든 공격실험에서 발효마늘분말이 포함된 사료를 섭취한 어류가 대조구와 비교하여 유의적으로 낮았다. 이번 연구의 결과를 볼 때, 사료 내 발효마늘분말의 첨가는 넙치에 있어서 V. anguillarum과 S. iniae에 대한 항병력 및 비특이적 면역반응을 향상시킨 것으로 사료된다.

Keywords

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