Journal of Oriental Neuropsychiatry (동의신경정신과학회지)

The Korean Society of Oriental Neuropsychiatry (대한한방신경정신과학회)
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Study on Effect to Serotonin Metabolism of Gamiguibi-tang on P815 Cell

가미귀비탕(加味歸脾湯)이 P815세포의 serotonin 대사 과정에 미치는 영향

  • No, Dong-Jin (Dept. of Oriental Neuropsychiatry College of Oriental Medicine, Dae-Jeon University) ;
  • Jung, In-Chul (Dept. of Oriental Neuropsychiatry College of Oriental Medicine, Dae-Jeon University)
  • 노동진 (대전대학교 한의과대학 신경정신과교실) ;
  • 정인철 (대전대학교 한의과대학 신경정신과교실)
  • Received : 2010.02.06
  • Accepted : 2010.03.20
  • Published : 2010.03.30
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Abstract

Objectives: This study was perfomed to investigate the antioxidant activity and serotonin activity of Gami-guibi-tang on P815 Mast Cell. Methods: The effects of Gami-guibi-tang on activation of TPH-1 mRNA and AAADC mRNA in P815 mast cell were investigated. The effect of Gami-guibi-tang on content of serotonin in P815 mast cell was investigated. The effects of Gami-guibi-tang on activation of DPPH radical scavenging and SOD in P815 mast cell were investigated. Results: 1. The Gami-guibi-tang increased SOD activity and DPPH radical scavenging activity. 2. The Gami-guibi-tang increased the intracellular concentration of serotoninin 60 ${\mu}g/ml$, 80 ${\mu}g/ml$ experiment group. 3. The Gami-guibi-tang increased menaingful the manifestation TPH mRNA. 4. The manifestation of AAADC and MAO mRNA have not made menaingful changes on Gami-guibi-tang. Conclusions: This experiment shows that Gami-guibi-tang had significant anti-oxidative effect. And Gami-guibi-tang increased the intracellular concentration of serotonin. Therefore, Gami-guibi-tang can be used by the medication of major depression disorder. But Study on mechanism of increased serotonin and clinical research of Gami-guibi-tang is suggested for future research.

Keywords

References

  1. American psychiatric association. DSM-IV-TR text rivision: Diagnostic and statistical manual for mental disorders, 4th ed. Washington DC: American psychiatric press. 2000
  2. 민성길. 제5판. 최신정신의학. 서울:일조각. 2008:274-99
  3. Piotr G, Janusz S, Małgorzata B, Antoni F, Elzbieta G. Lipid peroxidation and antioxidant protection in patients during acute depressive episodes and in remission after fluoxetine treatment. Pharmacological reports. 2009;61:436-47
  4. 이상용, 서원희. 鬱證과 우울증의 비교고찰. 대전대학교 한의학연구소 논문집. 1997;6(1):505-14
  5. 전국한의과대학 신경정신과 교과서편찬위원회. 한의신경정신과학. 경기도:집문당. 2007:256-65.
  6. 嚴用和. 濟生方. 北京:人民衛生出版社. 1980:117
  7. 薜己. 內科摘要. 江蘇:江蘇科學技術出版社. 1985:41-2.
  8. 문충모, 황의완. 歸脾湯의 항 Stress효과에 대한 실험적 고찰. 경희의학. 1986;2(3):388-9.
  9. 임동욱, 유동열. 加味歸脾湯이 면역조절작용에 미치는 영향. 대한한방부인과학회지. 1999;12(2):253-80
  10. 박동원, 박재우, 김진성, 류봉하, 류기원. 歸脾湯의 항피로효능에 관한 연구. 한방성인병학회지. 2000;6:162-73
  11. 박선동, 박현준, 주왕석. 歸脾湯 및 그 구성약물군이 항산화효과에 미치는 영향. 대한본초학회지. 2001;16:11-27.
  12. 許浚. 東醫寶鑑. 서울:여강출판사. 2001:125.
  13. Lowry OH, Rosebrough NJ, Farr AL, and Randall RJ. Protein measurement with the folin phenol reagent. J. Biol. Chem. 1951;193:265-75.
  14. Weissman MM, Bland RC, Canino GJ, Faravelli C, Greenwald S, Hwu HG, et al. Cross‐national epidemiology of major depression and bipolar disorder. JAMA. 1996;276:293-9 https://doi.org/10.1001/jama.276.4.293
  15. Nestler EJ, Barrot M, DiLeone RJ, Eisch AJ, Gold SJ, Monteggia LM. Neurobiology of depression. Neuron. 2002;34:13-25. https://doi.org/10.1016/S0896-6273(02)00653-0
  16. Meltzer CC, Smith G, DeKosky ST, Pollock BG, Mathis CA, Moore RY, et al. Serotonin in aging, late life depression, and Alzheimer's disease: the emerging role of functional imaging. Neuropsychopharmacology. 1998;18:407-30 https://doi.org/10.1016/S0893-133X(97)00194-2
  17. Lovenberg W, Bourgoin S, Hery F and Sjoerdsas A. Tryptophan hydroxylation; measurementin pineal gland, brain stem and carcinoid tumor. Science. 1967;155:217-9 https://doi.org/10.1126/science.155.3759.217
  18. Huh SO, Park DH, Cho JY, Joh TH, Son JH. A 6.1 kb 5'upstream region of the mouse tryptophan hydroxylase gene directs expression of E. coli lacZ to major serotonergic brain regions and pineal gland in transgenic mice. Mol. Brain Res. 1994;24:145-52 https://doi.org/10.1016/0169-328X(94)90126-0
  19. Hwang ES, Kim GH. Biomarkers of oxidative stress status of DNA, lipids, and proteins in vitro and in vivo cancer research. Toxicology. 2007;229(5):1-10
  20. Kupfer DJ, Frank E. The interaction of drug and psychotherapy in the long term treatment of depression. J. Affect Disord. 2001;62:131-7. https://doi.org/10.1016/S0165-0327(00)00357-8
  21. 김용구. 미래의 항우을제:어떠한 것들이 개발되고 있는가? 생물정신의학. 2004;11(1):14-25.
  22. 유영수. 鬱證의 정신의학적 고찰. 원광한의학. 1995;5(1):125-40
  23. 江克明. 簡明方劑辭典. 中國:上海科學技術出版社. 1989:298.
  24. Yu DH, Qiao SY, Zhao YM. Advances in study on bark of Albizzia julibrissin. Institute of Pharmacology and Toxicology. 2004;29(7):619-24
  25. 조창규, 이순곤, 이권해. MTT 분석법을 이용한 점액성 난소암세포주 RMUG sublines의 Cis-platinum 감수성에 관한 연구. 순천향대학논문집. 1993;16(4):1169-76.