Journal of Oriental Neuropsychiatry (동의신경정신과학회지)

The Korean Society of Oriental Neuropsychiatry (대한한방신경정신과학회)
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A Study on the Effects of the Electro-Acupuncture(EA) Stimulation after Global Ischemia(GI) on Changes in Bax and Caspase-3 Expression among Forebrain Apoptosis

뇌허혈 유발 후 전침자극이 전뇌세포사 중 Bax와 Caspase-3 발현 변화에 미치는 영향 연구

  • Choi, Jung-Hyun (Dept. of Physical Therapy, Nam-Seoul Univ of Korea) ;
  • Min, Kyoung-Ok (Dept. of Physical Therapy, Yong-In Univ of Korea) ;
  • Kim, Soon-Hee (Dept. of Physical Therapy, Yong-In Univ of Korea) ;
  • Kim, Dong-Il (Dept. of Oriental Medicine Graduate School of Dongguk Univ.) ;
  • Lee, Sang-Bin (Dept. of Physical Therapy, Nam-Seoul Univ of Korea) ;
  • An, Ho-Jung (Dept. of Physical Therapy, Dae-Won Univ College of Korea) ;
  • Kim, Ji-Sung (Dept. of Physical Therapy, Suwon Women's College of Korea) ;
  • Choi, Wan-Suk (Dept. of Physical Therapy, Po-Hang College of Korea) ;
  • Kim, Bo-Kyoung (Dept. of Physical Therapy, Gun-Jang College of Korea) ;
  • Song, Chi-Won (Div. of General Service, National Institute of Food & Drug Safety Evaluation)
  • 최정현 (남서울대학교 물리치료과) ;
  • 민경옥 (용인대학교 물리치료학과) ;
  • 김순희 (용인대학교 물리치료학과) ;
  • 김동일 (동국대학교한방병원 부인학과) ;
  • 이상빈 (남서울대학교 물리치료과) ;
  • 안호정 (대원대학 물리치료학과) ;
  • 김지성 (수원여자대학 물리치료학과) ;
  • 최완석 (포항대학 물리치료학과) ;
  • 김보경 (군장대학 물리치료학과) ;
  • 송치원 (식품의약품안전평가원 행정지원과)
  • Received : 2010.01.20
  • Accepted : 2010.03.09
  • Published : 2010.03.30
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Abstract

Objectives: The study aims to look into how the application of EA(Electro-acupuncture) in the initial step of ischemic brain injury affects the changes in Bax and Caspase-3 expression among forebrain apoptosis. Methods: It caused GI by using common carotid artery occlusion and conducted the test by dividing into the no applied EA group after 6 hours and 12 hours, the LI 4 EA group that applied EA to LI4, and GV 20 EA group that was applied to GV 20. After that, the following results were obtained by comparing Bax and Caspase-3 expression, which were apoptosis-related factors, with its application time through immunohistochemical staining after extracting brain of each group. Results: According to each group's Bax expression by the application time, the order of expression increase after 6 hours is shown to be the no applied EA group, LI 4 group, and GV 20 group. The stimulation after 12 hours was most lowly expressed in the GV 20 group, and the no applied EA group and LI 4 group showed similar level. Conclusions: EA stimulation in the initial step after ischemia seems to affect positively Bax and Caspase-3 expression that are index of forebrain apoptosis.

Keywords

References

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