Development and Reproduction (한국발생생물학회지:발생과생식)

The Korean Society of Developmental Biology (한국발생생물학회)
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Effects of EGF and PAF on the Hatching and Implantation of Peri-implantation Stage Embryos

  • Cheon, Yong-Pil (Division of Development and Physiology, School of Biosciences and Chemistry, Center for NanoBio Applied Technology, Institute for Basic Sciences, Sungshin Women's University)
  • Received : 2010.02.08
  • Accepted : 2010.03.07
  • Published : 2010.03.31
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Abstract

A fertilized oocyte can get the competence for implantation through cleavage and stage-specific gene expression. These are under the control of autonomous and exogenous regulators including physiological culture condition. Endogenous and exogenous growth factors are considered as critical regulators of cleaving embryos during travel the oviduct and uterus. In this study, an effort was made to evaluate comprehensively the quality of embryos for implantation, grown in media enriched with EGF and PAF. The study evaluated developmental rates on given time, blastulation and hatching rates, and adhesion rates. Developmental rates of blastocyst to the hatching stage were significantly high in PAF treated group compared to the control in a dose-dependent manner but not in EGF group. Implantation rates were significantly high both PAF and EGF in a dose-dependent manner. H7, a PKC inhibitor, blocked the process of hatching of the blastocysts but combined treatment of EGF and PAF enhanced the hatching and implantation of blastocsyts. Based on these results it is suggested that EGF and PAF support acquirement of implantation competence at blastocyst stage through a PKC pathway.

Keywords

References

  1. Bazer FW, Spencer TE, Johnson GA, Burghardt RC, Wu G (2009) Comparative aspects of implantation. Reproduction 138:195-209. https://doi.org/10.1530/REP-09-0158
  2. Cheon YP (2006) PAF regulate blastocyst development to hatching stage through PKC activity in the mouse. Reprod Dev Biol 30:75-79.
  3. Cheon YP, Li Q, Xu X, DeMayo FJ, Bagchi IC, Bagchi MK (2002) A genomic approach to identify novel progesterone receptor regulated pathways in the uterus during implantation. Mol Endocrinol 16:2853-2871. https://doi.org/10.1210/me.2002-0270
  4. Cho YK, Chae HD, Cheon YP, Kim CH, Kang BM, Chang YS, Mok JE (1998) The effect of epidermal growth factor on the preimplantation development, implantation and its receptor expression in mouse embryos. Kor J Obstet Gynecol 41:2839-2848.
  5. Dadi TD, Li MW, Lioyd KC (2004) Expression levels of EGF, TGF$\alpha$, and EGFR are significantly reduced in preimplantation cloned mouse embryos. Cloning Stem Cells 6:267-283. https://doi.org/10.1089/clo.2004.6.267
  6. Dadi TD, Li MW, Lloyd KC (2009) Decreased growth factor expression through RNA interference inhibits development of mouse preimplantation embryos. Comp Med 59:331-338.
  7. Dearn S, Rahman M, Lewis A, Ahmed Z, Eggo MC, Ahmed A (2000) Activation of platelet-activating factor (PAF) receptor stimulates nitric oxide (NO) release via protein kinase C-alpha in HEC-1B human endometrial epithelial cell line. Mol Med 6:37-49.
  8. Guillmotot M, Flechon JE, Leroy E (1993) Blastocyst development and implantation. In: Thibault C, Levasseur MC, Hunter RHF (eds.), Reproduction in Mammals and Man. Ellipses, Paris, pp 387-412.
  9. Harper MJK (1989) Platelet-activating factor; a paracrine factor in preimplantation stages of reproduction? Biol Reprod 40:907-913. https://doi.org/10.1095/biolreprod40.5.907
  10. Hayashi K, Burghardt RC, Bazer FW, Spencer TE (2007) WNTs in the ovine uterus: potential regulation of periimplantation ovine conceptus development. Endocrinology 148:3496-3506. https://doi.org/10.1210/en.2007-0283
  11. Hogan B, Beddington R, Costantini F, Lacy E (1994) Manipulating the Mouse Embryo a Laboratory Manual. 2nd ed. Cold Spring Harbor Laboratory Press, New York, pp 45-190.
  12. Jin XL, Chandrakanthan V, Morgan HD, O'Neill C (2009) Preimplantation embryo development in the mouse requires the latency of TRP52 expression, which is induced by a ligand-activated PI3 kinase/AKT/MDM2- mediated signaling pathway. Biol Reprod 80:286-294. https://doi.org/10.1095/biolreprod.108.070102
  13. Jones GM, Trounson AO, Gardner DK, Kausche A, Lolatgis N, Wood C (1998) Evolution of a culture protocol for successful blastocyst development and pregnancy. Hum Reprod 13:169-177. https://doi.org/10.1093/humrep/13.1.169
  14. Kang BM, Cheon YP, Kim JY, Kim JH, Lee JY, Chae Hd, Kim CH, Chang YS, Mok JE (1997) Effect of embryo number and incubation volume on the development of pre-and post-implantation mouse embryos in vitro. Kor J Fertil Steril 24:377-383.
  15. Kim JJ, Fortier MA (1995) Cell type specificity and protein kinase C dependency on the stimulation of prostaglandin $E_2$ and prostaglandin $F_2\alpha$ production by oxytocin and platelet-activating factor in bovine endometrial cells. J Reprod Fertil 103:239-247. https://doi.org/10.1530/jrf.0.1030239
  16. Kurzawa R, Glabowski W, Wenda-Rozewicka L (2001) Evaluation of mouse preimplantation embryos cultured in media enriched with insulin-like growth factors I and II, epidermal growth factor and tumor necrosis factor alpha. Folia Histochem Cytobiol 39:245-251.
  17. Lu DP, Chandrakanthan V, Cahana A, Ishii S, O'Neill C (2004) Trophic signals acting via phosphatidylinositol- 3-kinase are required for normal preimplantation mouse embryo development. J Cell Sci 117:1567-1576. https://doi.org/10.1242/jcs.00991
  18. O'Neill C (1987) Embryo derived platelet activating factor: pre-implantation embryo mediator of maternal recognition of pregnancy. Domestic Animal Endocrinol 4:69-86. https://doi.org/10.1016/0739-7240(87)90001-4
  19. O'Neill C (2005) The role of PAF in embryo physiology. Hum Reprod Update 11:215-228. https://doi.org/10.1093/humupd/dmi003
  20. Paria BC, Dey SK (1990) Preimplantation embryo development in vitro: cooperative interactions among embryos and role of growth factors. Proc Natl Acad Sci USA 87:4756-4760. https://doi.org/10.1073/pnas.87.12.4756
  21. Peters DD, Marschall S, Mahabir E, Boersma A, Heinzmann U, Schmidt J, Hrave de Angelis M (2006) Risk assessment of mouse hepatitis virus infection in vitro fertilization and embryo transfer by the use of zonaintact and laser-microdissected oocytes. Biol Reprod 74:246-252. https://doi.org/10.1095/biolreprod.105.045112
  22. Pratten MK, Brooke AM, Broome SC, Beck F (1988) The effect of epidermal growth factor, insulin and transferring on the growth-promoting properties of serum depleted by repeated culture of postimplantation mouse embryos. Development 104:137-145.
  23. Pribenszky C, Losonczi E, Molnar M, Lang Z, Matyas S, Rajczy K, Molnar K, kovacs P, Nagy P, Conceicao j, Vajta G (2010) Prediction of in-vitro developmental competence of early cleavage-stage mouse embryos with compact time-lapse equipment. Reprod Biomed Online 20:371-379. https://doi.org/10.1016/j.rbmo.2009.12.007
  24. Purnell ET, Warner CM, Kort HI, Mitchell-Leef D, Elsner CW, Shapiro DB, Massey JB, Roudebush WE (2006) Influence of the preimplantation embryo development (Ped) gene on embryonic platelet-activating factor (PAF) levels. J Assist Reprod Genet 23:269-273. https://doi.org/10.1007/s10815-006-9039-z
  25. Rankin S, Rozengurt E (1994) Platelet-derived growth factor modulation of focal adhesion kinase (p125FAK) and paxillin tyrosine phosphorylation in Swiss 3T3 cells. Bell-shaped dose response and cross-talk with bombesin. J Biol Chem 269:704-710.
  26. Richter KS (2008) The importance of growth factors for preimplantation embryo development and in-vitro culture. Curr Opin Obstet Gynecol 20:292-304. https://doi.org/10.1097/GCO.0b013e3282fe743b
  27. Romero FJ, Liopis J, Felipo V, Minana Md, Roma J, Grisolia S (1992) H7, a protein kinase C inhibitor, increases the glutathione content of neuroblastoma cells. FEBS Letter 303:19-21. https://doi.org/10.1016/0014-5793(92)80468-V
  28. Ryan JP, Spinks NR, O'Neill C, Wales RG (1990) Implantation potential and fetal viability of mouse embryos cultured in media supplemented with platelet-activating factor. J Reprod Fertil 89:309-315. https://doi.org/10.1530/jrf.0.0890309
  29. Schwall RH, Chang LY, Godowski PJ, Kahn DW, Hillan KJ, Bauer KD, Zioncheck TF (1996) Heparin induces dimerization and confers proliferative activity onto the hepatocyte growth factor antagonists NK1 and NK2. J Cell Biol 133:709-718. https://doi.org/10.1083/jcb.133.3.709
  30. Seshagiri PB, Sen Roy S, Sireesha G, Rao RP (2009) Cellular and molecular regulation of mammalian blastocyst hatching. J Reprod Immunol 83:79-84. https://doi.org/10.1016/j.jri.2009.06.264
  31. Stojanov T, O'Neill C (1999) Ontogeny of expression of a receptor for platelet-activating factor in mouse preimplantation embryos and the effects of fertilization and culture in vitro. Biol Reprod 60:674-682. https://doi.org/10.1095/biolreprod60.3.674
  32. Van der Auwera I, D'Hooghe T (2001) Superovulation of female mice delays embryonic and fetal development. Hum Reprod 16:1237-1243. https://doi.org/10.1093/humrep/16.6.1237
  33. Velasquez LA, Maisey K, Fernandez R, Valdes D, Cardenas H, Imarai M, Delgado J, Aguilera J, Croxatto HB (2001) PAF receptor and PAF acetylhydrolase expression in the endosalpinx of the human fallopian tube: possible role of embryo-derived PAF in the control of embryo transport to the uterus. Hum Reprod 16:1583-1587. https://doi.org/10.1093/humrep/16.8.1583
  34. Wang Y, Wu J, Wang Z (2006) Akt binds to and phosphorylates phospholipase C-(gamma)1 in response to epidermal growth factor. Mol Biol Cell 17:2267-2277. https://doi.org/10.1091/mbc.E05-10-0918
  35. Wollenhaupt K, Welter H, Einspanier R, Manabe N, Brussow KP (2004) Expression of epidermal growth factor receptor (EGF-R), vascular endothelial growth factor receptor (VEGF-R) and fibroblast growth factor receptor (FGF-R) systems in porcine oviduct and endometrium during the time of implantation. J Reprod Dev 50:269-278. https://doi.org/10.1262/jrd.50.269
  36. Yeo CX, Gilchrist RB, Thompson JG, Lane M (2008) Exogenous growth differentiation factor 9 in oocyte maturation media enhances subsequent embryo development and fetal biability in mice. Hum Reprod 23:67-73.