Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Anti-obesity Effects and Mechanism of Original and Modified Gambejaeseup-tang in Female Rats with Diet-induced Obesity

고지방식이로 유발된 비만 백서에서 가미감비제습탕이 비만 유발에 미치는 영향 및 기전 연구

  • Park, Sun-Min (Department of Food and Nutrition, College of Natural Science, Basic Science Institutes, Hoseo University) ;
  • Kim, Da-Sol (Department of Food and Nutrition, College of Natural Science, Basic Science Institutes, Hoseo University) ;
  • Kang, Sun-A (Department of Food and Nutrition, College of Natural Science, Basic Science Institutes, Hoseo University) ;
  • Lee, Jung-Bok (Sonimedi)
  • 박선민 (호서대학교 자연과학대학 기초과학연구소 식품영양학과) ;
  • 김다솔 (호서대학교 자연과학대학 기초과학연구소 식품영양학과) ;
  • 강선아 (호서대학교 자연과학대학 기초과학연구소 식품영양학과) ;
  • 이정복 (소니메디(주))
  • Received : 2010.07.09
  • Accepted : 2010.08.05
  • Published : 2010.08.25
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Abstract

Gambejaeseup-tang (GBJST) have recently been used as an anti-obesity herbal medicine but their effect and mechanism of action have not been studied. We modified ingredients of GBJST based on the previous experiments about exploring herbs to suppress triglyceride accumulation in 3T3-L1 adipocytes. We investigated the effects of modified GBJST on energy, glucose and lipid homeostasis using female rats with diet-induced obesity and their action mechanism was also determined. Rats fed a high-fat diet (HFD) were divided into 3 groups: rats in each group received 0.2 or 2 g water extracts of modified GBJST (L-GBJST or H-GBJST) or 2 g cellulose per kg body weight (a negative control) on a daily basis. A further group was fed a low-fat diet (LFD) as a positive control. We found that modified GBJST dose-dependently decreased body weight and mesenteric and retroperitoneal fat more than the control. This decrease was due to the reduction in energy intake and the increase of energy expenditure. HFD increased fat oxidation more than LFD and modified GBJST further increased fat oxidation as a major energy source more than the control in a dose-dependent manner. In addition, H-GBJST improved glucose tolerance without changing serum insulin levels during an oral glucose tolerance test. H-GBJST also suppressed the increase of serum total and LDL cholesterol and triglyceride levels by HFD. In conclusion, modified GBJST have a good anti-obesity effect by decreasing energy intake and increasing energy expenditure mainly as fat in female rats with diet-induced obesity. It also improves glucose tolerance and lipid metabolism.

Keywords

References

  1. Lee, Y.E., Park, J.E., Hwang, J.Y., Kim, W.Y. Comparison of health risks according to the obesity types based upon BMI and waist circumference in Korean adults: The 1998-2005 Korean National Health and Nutrition Examination Surveys. Kor J Nutri. 42: 631-638, 2009. https://doi.org/10.4163/kjn.2009.42.7.631
  2. Taubes, G. Weight increases worldwide? Science 280: 1368, 2000.
  3. Ministry of Health & Welfare. Report on the 2001 National Health and Nutrition Survey. Seoul, 2002.
  4. Bjorbaek, C., Kahn, B.B. Leptin signaling in the central nervous system and the periphery. Recent Prog Horm Res. 59: 305-331, 2004.
  5. Sahu, A. A hypothalamic role in energy balance with special emphasis on leptin. Endocrinology 145: 2613-2620, 2004. https://doi.org/10.1210/en.2004-0032
  6. Richard, D. Energy expenditure: a critical determinant of energy balance with key hypothalamic controls. Minerva Endocrinol. 32: 173-183, 2007.
  7. Morgan, D.A., Thedens, D.R., Weiss, R., Rahmouni, K. Mechanisms mediating renal sympathetic activation to leptin in obesity. Am J Physiol Regul Integr Comp Physiol. 295: R1730-R1736, 2008. https://doi.org/10.1152/ajpregu.90324.2008
  8. Sahu, A. Leptin signaling in the hypothalamus: emphasis on energy homeostasis and leptin resistance. Front Neuroendocrinol. 24(4):225-253, 2003. https://doi.org/10.1016/j.yfrne.2003.10.001
  9. Cherniack, E.P. Potential applications for alternative medicine to treat obesity in an aging population. Altern Med Rev. 13(1):34-42, 2008.
  10. Asghar, M., Monjok, E., Kouamou, G., Ohia, S.E., Bagchi, D., Lokhandwala, M.F. Super CitriMax (HCA-SX) attenuates increases in oxidative stress, inflammation, insulin resistance, and body weight in developing obese Zucker rats. Mol Cell Biochem. 304(1-2):93-99, 2007. https://doi.org/10.1007/s11010-007-9489-3
  11. Kaplan, L.M. Pharmacologic therapies for obesity. Gastroenterol Clin North Am. 39(1):69-79, 2010. https://doi.org/10.1016/j.gtc.2010.01.001
  12. Choussein, S., Makri, A.A., Frangos, C.C., Petridou, E.T., Daskalopoulou, S.S. Effect of antiobesity medications in patients with type 2 diabetes mellitus. Diabetes Obes Metab. 11(7):641-664, 2009. https://doi.org/10.1111/j.1463-1326.2008.01026.x
  13. Dulloo, A.G. Herbal simulation of ephedrine and caffeine in treatment of obesity. Int J Obes Relat Metab Disord. 26(5):590-592, 2002. https://doi.org/10.1038/sj.ijo.0802036
  14. 한방임상처방대전. 동국대부속한방병원 원내처방편, 미술공론사, 서울, p 146, 1999.
  15. Lusk, G. Analysis of the oxidation of mixtures of carbohydrate and fat. J Biol Chem. 59: 41, 1924.
  16. Niwa, H., Ogawa, Y., Kido, Y., Abe, Y., Kobayashi, M., Mori, T., Tanaka, T. The rate of lipid oxidation in septic rat models. Jpn J Surg. 19: 439-445, 1989. https://doi.org/10.1007/BF02471625
  17. Friedewald, W.T., Levy, R.I., Fredrickson, D.S. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem. 18(6):499-502, 1972.
  18. Park, S., Hong, S.M., Ahn, I.S. Long-term ICV infusion of insulin, but not glucose, modulates body weight and hepatic insulin sensitivity through modifying hypothalamic insulin signaling pathway in type 2 diabetic rats. Neuroendocrinology 89: 387-399, 2009. https://doi.org/10.1159/000197974
  19. Berube-Parent, S., Pelletier, C., Dore, J., Tremblay, A. Effects of encapsulated green tea and Guarana extracts containing a mixture of epigallocatechin-3-gallate and caffeine on 24 h energy expenditure and fat oxidation in men. Br J Nutr. 94(3):432-436, 2005. https://doi.org/10.1079/BJN20051502
  20. Boschmann, M., Thielecke, F. The effects of epigallocatechin-3-gallate on thermogenesis and fat oxidation in obese men: a pilot study. J Am Coll Nutr. 26(4):389S-395S, 2007. https://doi.org/10.1080/07315724.2007.10719627
  21. Diepvens, K., Westerterp, K.R., Westerterp-Plantenga, M.S. Obesity and thermogenesis related to the consumption of caffeine, ephedrine, capsaicin, and green tea. Am J Physiol Regul Integr Comp Physiol. 292(1):R77-85, 2007. https://doi.org/10.1152/ajpregu.00832.2005
  22. Hursel, R., Westerterp-Plantenga, M.S. Thermogenic ingredients and body weight regulation. Int J Obes (Lond). 34(4):659-669, 2010. https://doi.org/10.1038/ijo.2009.299
  23. Kawada, T., Watanabe, T., Takaishi, T., Tanaka, T., Iwai, K. Capsaicin-induced beta-adrenergic action on energy metabolism in rats: influence of capsaicin on oxygen consumption, the respiratory quotient, and substrate utilization. Proc Soc Exp Biol Med. 183(2):250-256, 1986. https://doi.org/10.3181/00379727-183-42414
  24. Park, S., Ahn, I.S., Kim, D.S., Kang, S., Kwon, D.Y., Yang, H.J. Anti-obesity effects of Tae-Um-Jo-Wee-Tang and Do-Dam-Tang in female rats with diet-induced obesity. J Appl Biol Chem. 53(1):44-50, 2010. https://doi.org/10.3839/jabc.2010.008
  25. Bruce, K.D., Byrne, C.D. The metabolic syndrome: common origins of a multifactorial disorder. Postgrad Med J. 85(1009):614-621, 2009. https://doi.org/10.1136/pgmj.2008.078014