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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Enhancement of HIV-1 Tat fusion protein transduction efficiency by bog blueberry anthocyanins

  • Lee, Sun-Hwa (Department of Biomedical Science & Research Institute of Bioscience and Biotechnology, Hallym University) ;
  • Jeong, Hoon-Jae (Department of Biomedical Science & Research Institute of Bioscience and Biotechnology, Hallym University) ;
  • Kim, Dae-Won (Department of Biomedical Science & Research Institute of Bioscience and Biotechnology, Hallym University) ;
  • Sohn, Eun-Jeong (Department of Biomedical Science & Research Institute of Bioscience and Biotechnology, Hallym University) ;
  • Kim, Mi-Jin (Department of Biomedical Science & Research Institute of Bioscience and Biotechnology, Hallym University) ;
  • Kim, Duk-Soo (Department of Anatomy, College of Medicine, Soonchunhyang University) ;
  • Kang, Tae-Cheon (Department of Anatomy & Neurobiology, College of Medicine, Hallym University) ;
  • Lim, Soon-Sung (Department of Food Science and Nutrition & RIC Center, Hallym University) ;
  • Kang, Il-Jun (Department of Food Science and Nutrition & RIC Center, Hallym University) ;
  • Cho, Sung-Woo (Department of Biochemistry and Molecular Biology, University of Ulsan College of Medicine) ;
  • Lee, Kil-Soo (Department of Biomedical Science & Research Institute of Bioscience and Biotechnology, Hallym University) ;
  • Park, Jin-Seu (Department of Biomedical Science & Research Institute of Bioscience and Biotechnology, Hallym University) ;
  • Eum, Won-Sik (Department of Biomedical Science & Research Institute of Bioscience and Biotechnology, Hallym University) ;
  • Choi, Soo-Young (Department of Biomedical Science & Research Institute of Bioscience and Biotechnology, Hallym University)
  • Received : 2010.06.09
  • Accepted : 2010.07.22
  • Published : 2010.08.31
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Abstract

Though protein transduction domains (PTDs) are well known for the delivery of exogenous therapeutic proteins into living cells, the overall low efficiency of transduction is a serious obstacle. We investigated the effect of bog blueberry anthocyanins (BBA) on protein transduction efficiency and found that BBA enhanced the transduction efficiencies of Tat-SOD fusion protein into HeLa cells and mice skin. The enzymatic activities in the cells and skin tissue in the presence of BBA were markedly increased compared to controls. Further, BBA did not demonstrate any cell toxicity at various concentrations. Although the mechanism is not fully understood, we suggest that BBA might alter the conformation of the membrane, which would indicate that BBA can be used as a protein transduction enhancer for the efficient delivery of therapeutic proteins for a variety of disorders.

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References

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