Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
권호 표지
DOI QR코드

DOI QR Code

Molecular Analysis of Microbial Community in Soils Cultivating Bt Chinese Cabbage

분자생물학적 분석을 통한 Bt 배추의 토양미생물상 영향 비교평가

  • 손수인 (농촌진흥청 국립농업과학원) ;
  • 오영주 (농촌진흥청 국립농업과학원) ;
  • 오성덕 (농촌진흥청 국립농업과학원) ;
  • 김민경 (농촌진흥청 국립농업과학원) ;
  • 류태훈 (농촌진흥청 국립농업과학원) ;
  • 이기종 (농촌진흥청 국립농업과학원) ;
  • 서석철 (농촌진흥청 국립농업과학원) ;
  • 백형진 (농촌진흥청 국립농업과학원) ;
  • 박종석 (농촌진흥청 국립농업과학원)
  • Received : 2010.09.14
  • Accepted : 2010.09.24
  • Published : 2010.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

The aim of this study was to investigate the possible impact of Bt Chinese cabbage on the soil microbial community. Microbial communities were isolated from the rhizosphere of one Bt Chinese cabbage variety and four varieties of conventional ones and were subjected to be analyzed using both culture-dependent and molecular methods. The total counts of bacteria, fungi, and actinomycetes in the rhizosphere of transgenic and conventional Chinese cabbages were observed to have an insignificant difference. Denaturing gradient gel electrophoresis (DGGE) analysis of PCR-amplified 16S rRNA genes revealed that the bacterial community structures were very similar to each other and this genetic stability of microbial communities was maintained throughout the culture periods. Analysis of dominant isolates in the rhizosphere of transgenic and conventional Chinese cabbages showed that the dominant isolates from the soil of transgenic Chinese cabbage belonged to the Bacilli and Alphaproteobacteria, while the dominant isolates from the soil of conventional cabbage belonged to the Holophagae and Planctomycetacia, respectively. These results indicate that the Bt transgenic cabbage has no significant impact on the soil microbial communities.

본 연구의 목적은 Bt 배추의 토양미생물 군집에 미치는 영향을 조사하기 위한 것이다. 토양미생물 조사에 앞서 토양 화학성분을 분석한 결과, Bt 배추와 일반품종 배추 근권토양간 화학성분의 유의성 있는 차이는 없는 것으로 조사되었다. 생육 최대 성숙기의 토양미생물 군집밀도를 조사했을 때 Bt 배추 근권토양의 미생물 군집밀도 범위가 일반 배추 근권토양의 미생물 군집밀도 범위내에 속하는 것으로 나타났다. 월별 DGGE 분석결과 일반품종 배추 근권토양 미생물 군집에 비해 Bt 배추 근권토양 미생물 군집의 변이는 없었다. 형질전환 벡터 유래 유전자부위를 이용하여 유전자의 수평이동성을 조사했을 때 Bt 근권토양 유래 DNA에서는 벡터 유래 유전자가 검출되지 않았다.

Keywords

References

  1. Cho, B.H., Cho, S.J., Park, C.S., Um, D.S., 2002, Soil science. Hyangmoonsa Press.
  2. Cho, H.S., Cao J., Ren, J.P., Earle, E.D., 2001, Control of Lepidopteran insect pests in transgenic Chinese cabbage (Brassica rapa ssp. pekinensis) transformed with a synthetic Bacillus thuringiensis cry1C gene. Plant Cell Rep. 20, 1-7. https://doi.org/10.1007/s002990000278
  3. Grayston, S. J., Griffith, G. S., Mawdsley, J. L., Campbell, C. D., Bardgett, R. D., 2001, Accounting for variability in soil microbial communities of temperate upland grassland ecosystems. Soil Biol Biochem 33, 679-682. https://doi.org/10.1016/S0038-0717(00)00215-7
  4. Icoz, I., Saxena, D., Andow, D.A., Zwahlen, C., Stotzky, G., 2008, Microbial populations and enzyme activities in soil in situ under transgenic corn expressing cry proteins from Bacillus thuringiensis. J. Environ. Qual. 37(2), 647-662. https://doi.org/10.2134/jeq2007.0352
  5. James, C., 2008, The global status of commercialized biotech/GM crops: 2009. ISAAA Briefs No. 41, Ithaka, NY.
  6. Kim, M.C., Ahn, J.H., Shin, H.C., Kim, T., Ryu, T.H., Kim, D.H., Song, H.G., Lee, G.H., Ka, J.O., 2008, Molecular analysis of bacterial community structures in paddy soils for environmental risk assessment with two varieties of genetically modified rice, Iksan 483 and Milyang 204. J. Microbiol Biotechnol. 18(2), 207-218.
  7. Kim, Y.J., Whang, K.S., 2007 Phylogenetic characteristics of viable but nonculturable bacterial populations in a pine mushroom (Tricholloma matcutake) forest soil. The Korean J. of Microbiol. 43, 201-209.
  8. Mendelsohn, M., Kough, J., Vaituzis, Z., Matthews, K., 2003, Are Bt safe? Nat. Biotechnol. 21(9), 1003-1009. https://doi.org/10.1038/nbt0903-1003
  9. Nap, J.P., Bijvoet, J., Stiekema, W.J., 1992, Biosafety of kanamycin-resistant transgenic plants. Transgenic Res. 1, 239-249. https://doi.org/10.1007/BF02525165
  10. Nielsen, K.M., Townsend, J.P., 2004, Monitoring and modeling horizontal gene transfer. Nat. Biotechnol. 22(9), 1110-1114. https://doi.org/10.1038/nbt1006
  11. Stewart, C.N., Jr., Halfhill, M.D., Warwick, S.I., 2003, Transgene introgression from genetically modified crops to their wild relatives. Nat. Rev. Gen. 4, 806-817. https://doi.org/10.1038/nrg1179
  12. de Vries, J., Wackernagel, W., 2004, Microbial horizontal gene transfer and the DNA release from transgenic crop plants. Plant and Soil 266, 91-104. https://doi.org/10.1007/s11104-005-4783-x

Cited by

  1. Assessment of Microbial Community in Paddy Soils Cultivated with Bt and Nakdong Rice vol.45, pp.5, 2012, https://doi.org/10.7745/KJSSF.2012.45.5.829
  2. Effects of Protox Herbicide Tolerance Rice Cultivation on Microbial Community in Paddy Soil vol.32, pp.2, 2013, https://doi.org/10.5338/KJEA.2013.32.2.95
  3. Effects of Long-Term Fertilization on Microbial Diversity in Upland Soils Estimated by Biolog Ecoplate and DGGE vol.47, pp.6, 2014, https://doi.org/10.7745/KJSSF.2014.47.6.451
  4. Assessment of Substantial Equivalence and Environmental Risk for Event Selection of Genetically Modified Chrysanthemum vol.32, pp.4, 2014, https://doi.org/10.7235/hort.2014.13149
  5. Evaluation and Assessment of Biosafety for Bacillus thuringiensis (Bt)-transgenic Rice: Responses of Daphnia magna Fed on Bt-transgenic Rice Variety vol.54, pp.4, 2011, https://doi.org/10.3839/jabc.2011.048
  6. Effects of Transgenic Soybean Cultivation on Soil Microbial Community in the Rhizosphere vol.30, pp.4, 2011, https://doi.org/10.5338/KJEA.2011.30.4.466
  7. Effect on Insecticide Susceptibility of Lissorhoptrus oryzophilus Fed on Carotenoid-Biofortified Rice Variety vol.31, pp.3, 2012, https://doi.org/10.5338/KJEA.2012.31.3.286