Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
권호 표지

The Effects of Euonymi Ligunum Suberalatum Phamacopuncture on Nitric Oxide and Interleukin-6 Production in Macrophage

귀전우(鬼箭羽)약침액이 대식세포의 Nitric Oxide와 Interleukin-6의 생성에 미치는 영향

  • Jeon, Jae-Cheon (Department of Acupuncture & Moxibustion, Chung-ju Oriental Medical Hospital, College of Oriental medicine, Semyung University) ;
  • Lee, Eun-Yong (Department of Acupuncture & Moxibustion, Chung-ju Oriental Medical Hospital, College of Oriental medicine, Semyung University)
  • 전재천 (세명대학교 부속충주한방병원 침구과) ;
  • 이은용 (세명대학교 부속충주한방병원 침구과)
  • Received : 2010.07.28
  • Accepted : 2010.08.07
  • Published : 2010.08.20
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Abstract

Objectives : The objective of this study is to study the effects of Euonymi Lignum Suberatatum pharmacopuncture solution on NO and IL-6 production in macrophage. Methods : At first, the RAW 264.7 macrophage was subclutured. In order to evaluate cytotoxicity, MTT assay performed. Then, the cell was induced by LPS, INF-$\gamma$ and Experimental groups were divided into five(Normal, Control, Euonymi Lignum Suberatatum 100, 200, $300{\mu}g/m{\ell}$). Then Euonymi Lignum Suberatatum pharmacopuncture solution was put into cell. We measured IL-6, iNOS, NO. Results : The cytotoxic effect of Euonymi Lignum Suberatatum pharmacopuncture solution in RAW 264.7 macrophage was not appeared. $300{\mu}g/m\ell$ Euonymi Lignum Suberatatum pharmacopuncture solution inhibited IL-6 production in LPS, INF-$\gamma$-stimulated RAW 264.7 macrophages significantly. Euonymi Lignum Suberatatum pharmacopuncture solution inhibited iNOS revelation in LPS, INF-$\gamma$-stimulated RAW 264.7 macrophages. All group of Euonymi Lignum Suberatatum pharmacopuncture solution inhibited NO production in LPS, INF-$\gamma$-stimulated RAW 264.7 macrophages significantly. Conclusions : Our study demonstrated that Euonymi Lignum Suberatatum pharmacopuncture solution had an inhibition effect on NO production, iNOS revelation, IL-6 production. So Euonymi Lignum Suberatatum pharmaco puncture solution may have an Anti-inflammation effect.

Keywords

References

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