Journal of the Korean Association of Oral and Maxillofacial Surgeons

The Korean Association of Oral and Maxillofacial Surgeons (대한구강악안면외과학회)
권호 표지
DOI QR코드

DOI QR Code

Isolation and characterization of human dental tissue-derived stem cells in the impacted wisdom teeth: comparison of dental follicle, dental pulp, and root apical papilla-derived cells

미성숙 매복지치의 치낭, 치수, 치근유두 조직에서 다능성 줄기세포의 분리와 특성화에 대한 연구

  • Song, Jung-Ho (Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University) ;
  • Park, Bong-Wook (Department Oral and Maxillofacial Surgery, School of Medicine and Institute of Health Science, Gyeongsang National University) ;
  • Byun, June-Ho (Department Oral and Maxillofacial Surgery, School of Medicine and Institute of Health Science, Gyeongsang National University) ;
  • Kang, Eun-Ju (College of Veterinary Medicine, Gyeongsang National University) ;
  • Rho, Gyu-Jin (College of Veterinary Medicine, Gyeongsang National University) ;
  • Shin, Sang-Hun (Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University) ;
  • Kim, Uk-Kyu (Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University) ;
  • Kim, Jong-Ryoul (Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University)
  • 송정호 (부산대학교 치의학전문대학원 구강악안면외과학교실) ;
  • 박봉욱 (경상대학교 의학전문대학원 치과학교실 구강악안면외과, 건강과학연구원) ;
  • 변준호 (경상대학교 의학전문대학원 치과학교실 구강악안면외과, 건강과학연구원) ;
  • 강은주 (경상대학교 수의과대학 수의산과학) ;
  • 노규진 (경상대학교 수의과대학 수의산과학) ;
  • 신상훈 (부산대학교 치의학전문대학원 구강악안면외과학교실) ;
  • 김욱규 (부산대학교 치의학전문대학원 구강악안면외과학교실) ;
  • 김종렬 (부산대학교 치의학전문대학원 구강악안면외과학교실)
  • Received : 2010.04.05
  • Accepted : 2010.05.20
  • Published : 2010.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Introduction: The first aim of this study was to isolate the dental tissue-derived stem cells from the dental follicle (DF), dental pulp (DP), and root apical papilla (RAP) of the extracted wisdom teeth. Second was to evaluate their characterization with the expressions of transcription factors and cell surface markers. Finally, their ability of the in vitro multi-lineage differentiations into osteogenic and adipogenic cells were compared, respectively. Materials and Methods: Dental tissues, including dental follicle, dental pulp, and root apical papilla, were separated in the extracted wisdom teeth. These three dental tissues were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with supplements, respectively. After passage 3, the homogeneous shaped dental tissue-derived cells were analyzed the expression of transcription factors (Oct-4, Nanog and Sox-2) and cell surface markers (CD44, CD90 and CD105) with reverse transcription polymerase chain reaction (RT-PCR) and fluorescence-activated cell sorting (FACS) analysis. In order to evaluate in vitro multi-lineage differentiations, the culture media were changed to the osteogenic and adipogenic induction mediums when the dental tissue-derived cells reached to passage 3. The characteristics of these three dental tissue-derived cells were compared with immunohistochemistry. Results: During primary culture, heterogenous and colony formatted dental tissue-derived cells were observed in the culture plates. After passage 2 or 3, homogenous spindle-like cells were observed in all culture plates. Transcription factors and mesenchymal stem cell markers were positively observed in all three types of dental tissue-derived cells. However, the quantity of expressed transcription factors was most large in RAP-derived cells. In all three types of dental tissue-derived cells, osteogenic and adipogenic differentiations were observed after treatment of specific induction media. In vitro adipogenic differentiation was similar among these three types of cells. In vitro osteogenic differentiation was most strongly and frequently observed in the RAP-derived cells, whereas rarely osteogenic differentiation was observed in the DP-derived cells. Conclusion: These findings suggest that three types of human dental tissue-derived cells from extracted wisdom teeth were multipotent mesenchymal stem cells, have the properties of multi-lineage differentiations. Especially, stem cells from root apical papilla (SCAP) have much advantage in osteogenic differentiation, whereas dental follicle cells (DFCs) have a characteristic of easy adipogenic differentiation.

Keywords

References

  1. Evans MJ, Kaufman MH. Establishment in culture of pluripotential cells from mouse embryos. Nature 1981;292:154-6. https://doi.org/10.1038/292154a0
  2. Wagers AJ, Weissman IL. Plasticity of adult stem cells. Cell 2004;116:639-48. https://doi.org/10.1016/S0092-8674(04)00208-9
  3. Mao JJ, Giannobile WV, Helms JA, Hollister SJ, Krebsbach PH, Longaker MT, et al. Craniofacial tissue engineering by stem cells. J Dent Res 2006;85:966-79. https://doi.org/10.1177/154405910608501101
  4. Almeida-Porada G, Porada C, Zanjani ED. Adult stem cell plasticity and methods of detection. Rev Clin Exp Hematol 2001;5: 26-41. https://doi.org/10.1046/j.1468-0734.2001.00027.x
  5. Temple S. Stem cell plasticity-building the brain of our dreams. Nat Rev Neurosci 2001;2:513-20. https://doi.org/10.1038/35081577
  6. Yamada Y, Boo JS, Ozawa R, Nagasaka T, Okazaki Y, Hata K, et al. Bone regeneration following injection of mesenchymal stem cells and fibrin glue with a biodegradable scaffold. J Craniomaxillofac Surg 2003;31:27-33. https://doi.org/10.1016/S1010-5182(02)00143-9
  7. Yamada Y, Ueda M, Naiki T, Nagasaka T. Tissue-engineered injectable bone regeneration for osseointegrated dental implants. Clin Oral Impl Res 2004;15:589-97. https://doi.org/10.1111/j.1600-0501.2004.01038.x
  8. Fuerst G, Tangl S, Gruber R, Gahleitner A, Sanroman F, Watzek G. Bone formation following sinus grafting with autogenous bone-derived cells and bovine bone mineral in minipigs: preliminary findings. Clin Oral Impl Res 2004;15:733-40. https://doi.org/10.1111/j.1600-0501.2004.01077.x
  9. Schimming R, Schmelzeisen R. Tissue-engineered bone for maxillary sinus augmentation. J Oral Maxillofac Surg 2004;62:724-9. https://doi.org/10.1016/j.joms.2004.01.009
  10. Gronthos S, Mankani M, Brahim J, Robey PG, Shi S. Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci USA 2000;97:13625-30. https://doi.org/10.1073/pnas.240309797
  11. Gronthos S, Brahim J, Li W, Fisher LW, Cherman N, Boyde A, et al. Stem cell properties of human dental pulp stem cells. J Dent Res 2002;81:531-5. https://doi.org/10.1177/154405910208100806
  12. Miura M, Gronthos S, Zhao M, Lu B, Fisher LW, Robey PG, et al. Stem cells from human exfoliated deciduous teeth. Proc Natl Acad Sci USA 2003;100:5807-12. https://doi.org/10.1073/pnas.0937635100
  13. Seo BM, Sonoyama W, Yamaza T, Coppe C, Kikuiri T, Akiyama K, et al. SHED repair critical-size calvarial defects in mice. Oral Dis 2008;14:428-34. https://doi.org/10.1111/j.1601-0825.2007.01396.x
  14. Huang AH, Chen YK, Lin LM, Shieh TY, Chan AW. Isolation and characterization of dental pulp stem cells from a supernumerary tooth. J Oral Pathol Med 2008;37:571-4. https://doi.org/10.1111/j.1600-0714.2008.00654.x
  15. Morsczeck C, Go ¨tz W, Schierholz J, Zeilhofer F, Ku ¨hn U, Mo ¨hl C, et al. Isolation of precursor cells (PCs) from human dental follicle of wisdom teeth. Matrix Biol 2005;24:155-65. https://doi.org/10.1016/j.matbio.2004.12.004
  16. Morsczeck C, Moehl C, Gotz W, Heredia A, Schaffer TE, Eckstein N, et al. In vitro differentiation of human dental follicle cells with dexamethasone and insulin. Cell Biol Int 2005;29:567- 75. https://doi.org/10.1016/j.cellbi.2005.03.020
  17. Reynolds AJ, Jahoda CA. Cultured human and rat tooth papilla cells induce hair follicle regeneration and fiber growth. Differentiation 2004;72:566-75. https://doi.org/10.1111/j.1432-0436.2004.07209010.x
  18. Seo BM, Miura M, Gronthos S, Bartold PM, Batouli S, Brahim J, et al. Investigation of multipotent postnatal stem cells from human periodontal ligament. Lancet 2004;364:149-55. https://doi.org/10.1016/S0140-6736(04)16627-0
  19. Sonoyama W, Liu Y, Fang D, Yamaza T, Seo BM, Zhang C, et al. Mesenchymal stem cell-mediated functional tooth regeneration in swine. PLoS ONE 2006;1:e79. https://doi.org/10.1371/journal.pone.0000079
  20. Morsczeck C, Vollner F, Saugspier M, Brandl C, Reichert TE, Driemel O, et al. Comparison of human dental follicle cells (DFCs) and stem cells from human exfoliated deciduous teeth (SHED) after neural differentiation in vitro. Clin Oral Investig [in press 2009 Jul 10].
  21. Kang EJ, Byun JH, Choi YJ, Maeng GH, Lee SL, Kang DH, et al. In vitro and in vivo osteogenesis of porcine skin-derived mesenchymal stem cell-like cells with a demineralized bone and fibrin scaffold. Tissue Eng Part A 2010;16:815-27. https://doi.org/10.1089/ten.tea.2009.0439
  22. Scholer HR, Ruppert S, Suzuki N, Chowdhury K, Gruss P. New type of POU domain in germ line-specific protein Oct-4. Nature 1990;344:435-9. https://doi.org/10.1038/344435a0
  23. Avilion AA, Nicolis SK, Pevny LH, Perez L, Vivian N, Lovell- Badge R. Multipotent cell lineages in early mouse development depend on SOX2 function. Genes Dev 2003;17:126-40. https://doi.org/10.1101/gad.224503
  24. Chambers I, Colby D, Robertson M, Nichols J, Lee S, Tweedie S, et al. Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells. Cell 2003;113:643-55. https://doi.org/10.1016/S0092-8674(03)00392-1
  25. Boyer LA, Lee TI, Cole MF, Johnstone SE, Levine SS, Zucker JP, et al. Core transcriptional regulatory circuitry in human embryonic stem cells. Cell 2005;122:947-56. https://doi.org/10.1016/j.cell.2005.08.020
  26. Kajahn J, Gorjup E, Tiede S, von Briesen H, Paus R, Kruse C, et al. Skin-derived human adult stem cells surprisingly share many features with human pancreatic stem cells. Eur J Cell Biol 2008; 87:39-46. https://doi.org/10.1016/j.ejcb.2007.07.004
  27. Carlin R, Davis D, Weiss M, Schultz B, Troyer D. Expression of early transcription factors Oct-4, Sox-2 and Nanog by porcine umbilical cord (PUC) matrix cells. Reprod Biol Endocrinol 2006; 4:8. https://doi.org/10.1186/1477-7827-4-8
  28. Izadpanah R, Joswig T, Tsien F, Dufour J, Kirijan JC, Bunnell BA. Characterization of multipotent mesenchymal stem cells from the bone marrow of rhesus macaques. Stem Cells Dev 2005;14:440-51. https://doi.org/10.1089/scd.2005.14.440
  29. Zhu Y, Liu T, Song K, Fan X, Ma X, Cui Z. Adipose-derived stem cell: a better stem cell than BMSC. Cell Biochem Funct 2008;26:664-75. https://doi.org/10.1002/cbf.1488
  30. Cheng PH, Snyder B, Fillos D, Ibegbu CC, Huang AH, Chan AW. Postnatal stem/progenitor cells derived from the dental pulp of adult chimpanzee. BMC Cell Biol 2008;9:20. https://doi.org/10.1186/1471-2121-9-20
  31. Dyce PW, Zhu H, Craig J, Li J. Stem cells with multilineage potential derived from porcine skin. Biochem Biophys Res Commun 2004;316:651-8. https://doi.org/10.1016/j.bbrc.2004.02.093
  32. Zhao M, Isom SC, Lin H, Hao Y, Zhang Y, Zhao J, et al. Tracing the stemness of porcine skin-derived progenitors (pSKP) back to specific marker gene expression. Cloning Stem Cells 2009;11: 111-22. https://doi.org/10.1089/clo.2008.0071
  33. Choi MJ, Byun JH, Kang EJ, Rho GJ, Kim UK, Kim JR, et al. Isolation of porcine multipotential skin-derived precursor cells and its multilineage differentiation. J Korean Assoc Oral Maxillofac Surg 2008;34:588-93.
  34. Park BW, Hah YS, Kim DR, Kim JR, Byun JH. Osteogenic phenotypes and mineralization of cultured human periosteal-derived cells. Arch Oral Biol 2007;52:983-9. https://doi.org/10.1016/j.archoralbio.2007.04.007
  35. Rho GJ, Kumar BM, Balasubramanian SS. Porcine mesenchymal stem cells - current technological status and future perspective. Front Biosci 2009;14:3942-61.
  36. Handa K, Saito M, Yamauchi M, Kiyono T, Sato S, Teranaka T, et al. Cementum matrix formation in vivo by cultured dental follicle cells. Bone 2002;31:606-11. https://doi.org/10.1016/S8756-3282(02)00868-2

Cited by

  1. Comparison of Immuno-Phenotypes of Stem Cells from Human Dental Pulp and Periodontal Ligament vol.25, pp.1, 2012, https://doi.org/10.1177/039463201202500115
  2. 과잉치 치수유래 줄기세포의 분화제 처리 기간에 따른 상아모세포 발현 특성 vol.42, pp.4, 2015, https://doi.org/10.5933/jkapd.2015.42.4.312
  3. 자가이식한 상악 견치의 생존율과 성공률에 영향을 주는 예후 인자 연구 vol.44, pp.3, 2010, https://doi.org/10.5933/jkapd.2017.44.3.317