Textile Science and Engineering (한국섬유공학회지)

The Korean Fiber Society (한국섬유공학회)
권호 표지

Enzymatic Hydrolysis of Chitosan Fiber Using Cellulase and Papain

셀룰라제와 파파인을 이용한 키토산 섬유의 효소가수분해

  • Lee, So-Hee (Department of Clothing and Textiles, Sookmyung Women's University) ;
  • Kim, Hye-Rim (Department of Clothing and Textiles, Sookmyung Women's University) ;
  • Lee, Bum-Hoon (KCRP, Kolon Industries Inc.) ;
  • Song, Wha-Soon (Department of Clothing and Textiles, Sookmyung Women's University)
  • 이소희 (숙명여자대학교 생활과학대학 의류학) ;
  • 김혜림 (숙명여자대학교 생활과학대학 의류학) ;
  • 이범훈 (코오롱 인더스트리(주) 중앙기술원) ;
  • 송화순 (숙명여자대학교 생활과학대학 의류학)
  • Received : 2010.03.08
  • Accepted : 2010.06.04
  • Published : 2010.06.30
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Abstract

The enzymatic processing conditions of chitosan nonwoven using cellulase and papain were optimized to determine the correlation between chitosan nonwoven and both enzymes. The hydrolytic activity and structural change in the chitosan fiber were evaluated. Chitosan hydrolysis by cellulase was maximized using 10% (o.w.f.) cellulase at $40^{\circ}C$ for 30 minutes under pH 5, whereas that by papain was optimized at a papain concentration of 10% (o.w.f.), and $60^{\circ}C$ for 60 minutes at pH 8. The degree of deacetylation of the chitosan decreased by 3.9% and 2.4% using cellulase and papain, respectively, which reduced slightly the level of acid dye adsorption of chitosan. DSC, XRD, FT-IR and GPC analysis confirmed no substantial changes in the molecular and crystalline structures of chitosan by the enzymatic treatment.

Keywords

Acknowledgement

Supported by : 한국연구재단

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