Journal of the Korean Association of Oral and Maxillofacial Surgeons

The Korean Association of Oral and Maxillofacial Surgeons (대한구강악안면외과학회)
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The bone regenerative effect of silk fibroin mixed with platelet-rich fibrin (PRF) in the calvaria defect of rabbit

가토의 두개 결손부에서의 실크 단백질과 platelet-rich fibrin (PRF)의 골형성 효과

  • Song, Ji-Young (Department of Oral and Maxillofacial Surgery, College of Dentistry, Gangneung-Wonju National University) ;
  • Kweon, Hae-Yong (National Academy of Agricultural Science, RDA) ;
  • Kwon, Kwang-Jun (Department of Oral and Maxillofacial Surgery, College of Dentistry, Gangneung-Wonju National University) ;
  • Park, Young-Wook (Department of Oral and Maxillofacial Surgery, College of Dentistry, Gangneung-Wonju National University) ;
  • Kim, Seong-Gon (Department of Oral and Maxillofacial Surgery, College of Dentistry, Gangneung-Wonju National University)
  • 송지영 (강릉원주대학교 치과병원 구강악안면외과) ;
  • 권해용 (농촌진흥청) ;
  • 권광준 (강릉원주대학교 치과병원 구강악안면외과) ;
  • 박영욱 (강릉원주대학교 치과병원 구강악안면외과) ;
  • 김성곤 (강릉원주대학교 치과병원 구강악안면외과)
  • Received : 2010.04.13
  • Accepted : 2010.05.28
  • Published : 2010.08.31
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Abstract

Introduction: This study evaluated the bone regenerative effect of silk fibroin mixed with platelet-rich fibrin (PRF) of a bone defect in rabbits. Materials and Methods: Ten New Zealand white rabbits were used for this study and bilateral round shaped defects were formed in the parietal bone (diameter: 8.0 mm). The silk fibroin mixed with PRF was grafted into the right parietal bone (experimental group). The left side (control group) was grafted only PRF. The animals were sacrificed at 4 weeks and 8 weeks. A micro-computerized tomography (${\mu}$CT) of each specimen was taken. Subsequently, the specimens were decalcified and stained for histological analysis. Results: The average value of plane film analysis was higher in the experimental group than in the control group at 4 weeks and 8weeks after surgery. However, the difference was not statistically significant.(P>0.05) The tissue mineral density (TMD) in the experimental group at 4 weeks after surgery was significantly higher than the control group.(P<0.05) Conclusion: Silk fibroin can be used as a scaffold of PRF for rabbit calvarial defect repair.

Keywords

References

  1. Buser D, Dula K, Hess D, Hirt HP, Belser UC. Localized ridge augmentation with autografts and barrier membranes. Periodontol 2000 1999;19:151-63.
  2. Laurie SW, Kaban LB, Mulliken JB, Murray JE. Donor-site morbidity after harvesting rib and iliac bone. Plast Reconstr Surg 1984;73:933-8. https://doi.org/10.1097/00006534-198406000-00014
  3. Sommers BN, Eisenstein SM. Donor site pain from the ilium. A complication of lumbar spine fusion. J Bone Joint Surg Br 1989;71:677-80.
  4. Younger EM, Chapman MW. Morbidity at bone graft donor sites. J Orthop Trauma 1989;3:192-5. https://doi.org/10.1097/00005131-198909000-00002
  5. Friedlaender GE, Horowitz MC. Immune responses to osteochondral allografts: nature and significance. Orthopedics 1992;15:1171-5.
  6. Carlson ER, Marx RE, Buck BE. The potential for HIV transmission through allogeneic bone. A review of risk and safety. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1995;80:17-23. https://doi.org/10.1016/S1079-2104(95)80010-7
  7. Bhanot S, Alex JC. Current applications of platelet gels in facial plastic surgery. Facial Plast Surg 2002;18:27-33. https://doi.org/10.1055/s-2002-19824
  8. Marx RE. Platelet-rich plasma (PRP): what is PRP and what is not PRP? Implant Dent 2001;10:225-8. https://doi.org/10.1097/00008505-200110000-00002
  9. Rodriguez A, Anastassov GE, Lee H, Buchbinder D, Wettan H. Maxillary sinus augmentation with deproteinated bovine bone and platelet rich plasma with simultaneous insertion of endosseous implants. J Oral Maxillofac Surg 2003;61:157-63. https://doi.org/10.1053/joms.2003.50041
  10. Tayapongsak P, O'Brien DA, Monteiro CB, Arceo-Diaz LY. Autologous fibrin adhesive in mandibular reconstruction with particulate cancellous bone and marrow. J Oral Maxillofac Surg 1994;52:161-5. https://doi.org/10.1016/0278-2391(94)90401-4
  11. Marx RE, Carlson ER, Eichstaedt RM, Schimmele SR, Strauss JE, Georgeff KR. Platelet-rich Plasma: growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1998;85:638-46. https://doi.org/10.1016/S1079-2104(98)90029-4
  12. Robiony M, Polini F, Costa F, Politi M. Osteogenesis distraction and platelet-rich plasma for bone restoration of the severely atrophic mandible: preliminary results. J Oral Maxillofac Surg 2002;60:630-5. https://doi.org/10.1053/joms.2002.33107
  13. Choukroun J, Diss A, Simonpieri A, Girard MO, Schoeffler C, Dohan SL, et al. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part V: histologic evaluations of PRF effects on bone allograft maturation in sinus lift. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006;101:299-303. https://doi.org/10.1016/j.tripleo.2005.07.012
  14. Altman GH, Diaz F, Jakuba C, Calabro T, Horan RL, Chen J, et al. Silk-based biomaterials. Biomaterials 2003;24: 401-16. https://doi.org/10.1016/S0142-9612(02)00353-8
  15. Huang J, Wong C, George A, Kaplan DL. The effect of genetically engineered spider silk-dentin matrix protein 1 chimeric protein on hydroxyapatite nucleation. Biomaterials 2007;28:2358-67. https://doi.org/10.1016/j.biomaterials.2006.11.021
  16. Meinel L, Fajardo R, Hofmann S, Langer R, Chen J, Snyder B, et al. Silk implants for the healing of critical size bone defects. Bone 2005;37:688-98. https://doi.org/10.1016/j.bone.2005.06.010
  17. Hirano Y, Mooney DJ. Peptide and protein presenting materials for tissue engineering. Adv Mater 2004;16:17-25. https://doi.org/10.1002/adma.200300383
  18. Dal Pra I, Freddi G, Minic J, Chiarini A, Armato U. De novo engineering of reticular connective tissue in vivo by silk fibroin nonwoven materials. Biomaterials 2005;26:1987-99. https://doi.org/10.1016/j.biomaterials.2004.06.036
  19. Stitzel J, Liu J, Lee SJ, Komura M, Berry J, Soker S, et al. Controlled fabrication of a biological vascular substitute. Biomaterials 2006;27:1088-94. https://doi.org/10.1016/j.biomaterials.2005.07.048
  20. Chan CK, Kumar TS, Liao S, Murugan R, Ngiam M, Ramakrishnan S. Biomimetic nanocomposites for bone graft applications. Nanomedicine (Lond) 2006;1:177-88. https://doi.org/10.2217/17435889.1.2.177
  21. Zhao J, Zhang Z, Wang S, Sun X, Zhang X, Chen J, et al. Apatite-coated silk fibroin scaffolds to healing mandibular border defects in canines. Bone 2009;45:517-27. https://doi.org/10.1016/j.bone.2009.05.026
  22. Li C, Vepari C, Jin HJ, Kim HJ, Kaplan DL. Electrospun silk-BMP-2 scaffolds for bone tissue engineering. Biomaterials 2006; 27:3115-24. https://doi.org/10.1016/j.biomaterials.2006.01.022
  23. Cao Y, Wang B. Biodegradation of silk biomaterials. Int J Mol Sci 2009;10:1514-24. https://doi.org/10.3390/ijms10041514
  24. Jang ES, Park JW, Kweon H, Lee KG, Kang SW, Baek DH, et al. Restoration of peri-implant defects in immediate implant installations by Choukroun platelet-rich fibrin and silk fibroin powder combination graft. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010;109:831-6. https://doi.org/10.1016/j.tripleo.2009.10.038
  25. Kim JY, Choi JY, Jeong JH, Jang ES, Kim AS, Kim SG, et al. Low molecular weight silk fibroin increases alkaline phosphatase and type I collagen expression in MG63 cells. BMB Rep 2010;43:52-6. https://doi.org/10.5483/BMBRep.2010.43.1.052

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