Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
권호 표지

Extraction and verification of highly immunologenic antigen for diagnosis of bovine brucellosis

소 브루셀라병 진단용 고면역원성 항원의 추출과 검증

  • Bae, Jae-Hyung (Cental branch of Gyeongnam Livestock Veterinary Promotion Research Institute) ;
  • Jo, Sang-Rae (Cental branch of Gyeongnam Livestock Veterinary Promotion Research Institute) ;
  • Jeong, Eun-Hui (Cental branch of Gyeongnam Livestock Veterinary Promotion Research Institute) ;
  • Jang, Eun-Hui (Cental branch of Gyeongnam Livestock Veterinary Promotion Research Institute) ;
  • Kim, Seong-Eun (Cental branch of Gyeongnam Livestock Veterinary Promotion Research Institute) ;
  • Kwon, Hui-Nyeong (Cental branch of Gyeongnam Livestock Veterinary Promotion Research Institute) ;
  • Park, Dong-Yeop (Cental branch of Gyeongnam Livestock Veterinary Promotion Research Institute) ;
  • Lee, Kuk-Cheon (Cental branch of Gyeongnam Livestock Veterinary Promotion Research Institute)
  • 배재형 (경남 축산진흥연구소 중부지소) ;
  • 조상래 (경남 축산진흥연구소 중부지소) ;
  • 정은희 (경남 축산진흥연구소 중부지소) ;
  • 장은희 (경남 축산진흥연구소 중부지소) ;
  • 김성은 (경남 축산진흥연구소 중부지소) ;
  • 권희녕 (경남 축산진흥연구소 중부지소) ;
  • 박동엽 (경남 축산진흥연구소 중부지소) ;
  • 이국천 (경남 축산진흥연구소 중부지소)
  • Received : 2010.04.09
  • Accepted : 2010.06.14
  • Published : 2010.06.30
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Abstract

Bovine brucellosis, an important zoonosis, is diagnosed with serological tests such as the RBT, TAT using inactivated whole bacterial cells or bacterial lipopolysaccharide (LPS) antigen in Korea. However, a strong cross-reaction between Brucella spp. and Yersinia enterocolitica O9 in these tests has seriously complicated the diagnosis of animal brucellosis because Brucella spp. shares common antigenic determinants with Y. enterocolitica O9 in the smooth LPS region. In this study, Brucella-field strains were isolated from Brucellapositive Hanwoo in Kimhae, Korea and outer membrane protein (omp) which has low cross-reaction with Y. enterocolitica O9 and high immunogenicity was extracted from the field strains Then we compared ELISA using the extract with RBT-TAT. Fifteen field strains were isolated from 47 supra-mammary-lymph nodes, which were collected from 18 farms. Isolation rate was 32%. Brucella-specific antigen was identified by performing SDS-PAGE or Western blotting on extracted omp with at 0.5% n-lauroylsarcosine One hundred and ninety-two serum-samples were used in the experiment: 142 negative and 50 positive samples verified by RBT-TAT. According to ELISA results, 127 samples were negative and 15 appeared positive among 142 negatives by RBT-TAT, while 42 samples were positive and 8 were negative among 50 positives by RBT-TAT. Therefore, it showed 89.4% of specificity and 84% of sensi-tivity. Through the current experiments, we could set up an ELISA based on the omp which has low cross-reaction and high immunogenicity and concluded that the omp could be a good material for accurate diagnosis of bovine brucellosis.

Keywords

References

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