Environmental Analysis Health and Toxicology

The Korean Society of Environmental Health and Toxicology (환경독성보건학회)
권호 표지

Evaluation of Antioxidant Activity of Platycodon grandiflorum

장생도라지 (Platycodon grandiflorum)의 항산화 활성 평가

  • Kim, Chung-Hyeon (College of Pharmacy and RCTCP, Chungnam National University) ;
  • Jung, Bong-Yong (College of Pharmacy and RCTCP, Chungnam National University) ;
  • Jung, Sun-Ki (College of Pharmacy and RCTCP, Chungnam National University) ;
  • Lee, Chul-Ho (Natural Medicine Research Center, KRIBB) ;
  • Lee, Hyun-Sun (Natural Medicine Research Center, KRIBB) ;
  • Kim, Bong-Hee (College of Pharmacy and RCTCP, Chungnam National University) ;
  • Kim, Sang-Kyum (College of Pharmacy and RCTCP, Chungnam National University)
  • 김충현 (충남대학교 약학대학 형질전환 복제돼지 센터) ;
  • 정봉용 (충남대학교 약학대학 형질전환 복제돼지 센터) ;
  • 정선기 (충남대학교 약학대학 형질전환 복제돼지 센터) ;
  • 이철호 (한국생명공학연구원, 천연물의약 연구센터) ;
  • 이현선 (한국생명공학연구원, 천연물의약 연구센터) ;
  • 김봉희 (충남대학교 약학대학 형질전환 복제돼지 센터) ;
  • 김상겸 (충남대학교 약학대학 형질전환 복제돼지 센터)
  • Published : 2010.03.31
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Abstract

Platycodon grandiflorum, Doraji as Korean name, is one of the most widely used traditional oriental medicine for bronchial diseases and also used as a folk remedy for geriatric diseases and inflammatory diseases. In recent studies, it has been reported that some effect of P. grandiflorum is derived from its antioxidant activity, although there is still a lack of evidence to establish its oxy-radical scavenging activity. In this study, total oxy-radical scavenging capacity (TOSC) assay was used to evaluate antioxidant activity of total extracts (T-PG), polysaccharide fraction (Po-PG), and saponin fraction (Sa-PG) isolated from P. grandiflorum against peroxyl radicals and peroxynitrites. And MTT assay was taken to assess cyto-protective effects of T-PG, Po-PG and Sa-PG in H4IIE cells treated with hydrogen peroxide and tert-butylhydroperoxide. In the TOSC assay, Sa-PG showed strong oxy-radical scavenging capacity compared with T-PG and Po-PG. In cell-based assay, T-PG and Po-PG protected cells from oxidative stress, but Sa-PG did not protect cells because of cytotoxicity of Sa-PG. These results suggest that the saponin components of P. grandiflorum have relatively strong antioxidant capacity and cytotoxicity in rat hepatoma cells.

Keywords

References

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