Applied Chemistry for Engineering (공업화학)

The Korean Society of Industrial and Engineering Chemistry (한국공업화학회)
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Carbon Dioxide Fixation using Spirulina Platensis NIES 39 in Polyethylene Bag

Spirulina Platensis NIES 39를 이용한 Polyethylene Bag 반응기에서의 이산화탄소 고정화

  • Kim, Young-Min (Department of Bioscience and Biotechnology, Collage of Engineering, Silla University) ;
  • Kim, Ji-Youn (Department of Bioscience and Biotechnology, Collage of Engineering, Silla University) ;
  • Lee, Sung-Mok (Department of Bioscience and Biotechnology, Collage of Engineering, Silla University) ;
  • Ha, Jong-Myung (Department of Bioscience and Biotechnology, Collage of Engineering, Silla University) ;
  • Kwon, Tae-Ho (Jeonju Biomaterials Institute) ;
  • Lee, Jae-Hwa (Department of Bioscience and Biotechnology, Collage of Engineering, Silla University)
  • 김영민 (신라대학교 공과대학 생명공학과) ;
  • 김지윤 (신라대학교 공과대학 생명공학과) ;
  • 이성목 (신라대학교 공과대학 생명공학과) ;
  • 하종명 (신라대학교 공과대학 생명공학과) ;
  • 권태호 (전주생물소재연구소) ;
  • 이재화 (신라대학교 공과대학 생명공학과)
  • Received : 2009.12.01
  • Accepted : 2010.02.23
  • Published : 2010.06.10
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Abstract

To replace current expensive photobioreactor, this study was conducted to develop low-cost photobioreactor made of polyethylene bag. In previous study, optimal culture conditions of Spirulina platensis NIES 39 have been established, and based on these, the study of biological carbon dioxide fixation has been conducted. The maximum growth was the biomass 2.677 g/L at conditions of 10% $CO_2$, 0.1 vvm. It was shown that $F_{CO_2}$ was 4.056 g $CO_2$/L and $R_{CO_2}$ was 0.312 g $CO_2$/L/day. But, compared with the data at conditions of 5% $CO_2$, 0.1 vvm, $FE_{CO_2}$ was shown 52.372% which is half of it. Regarding the effect of $CO_2$ following illumination, the growth revealed that the input conditions, for 10 min per 3 h, were excellent in the light. $CO_2$ in absent light. $CO_2$ concentration and flow rate were 5% $CO_2$, 0.1 vvm, respectively. Finally, the addition of $CO_2$ was ineffective in the absence of light.

현재의 값비싼 광생물반응기를 대체하기 위하여 비닐백을 소재로 한 보급형 광생물반응기를 개발하고자 본 연구를 실시하였다. 앞선 연구에서 Spirulina platensis NIES 39의 최적배양조건을 확립하였으며, 이를 토대로 하여 이산화탄소 고정화 연구를 실시하였다. 이산화탄소 농도 및 유속에 따른 성장은 10% $CO_2$, 0.1 vvm의 조건에서 가장 높은 2.677 g/L의 건조균체량을 나타냈으며, 이산화탄소 고정화량($F_{CO_2}$)은 4.056 g ${\cdot}{CO_2}$/L, 이산화탄소 고정화속도($R_{CO_2}$)는 0.312 g $CO_2$/L/day로 나타났다. 반면, 이산화탄소 고정화 효율($FE_{CO_2}$)의 경우 5% $CO_2$, 0.1 vvm의 조건에서 나타난 데이터에 비해서 그 절반 수준인 52.372%를 나타내었다. 그리고 빛의 유무에 따른 이산화탄소 주입효과를 알아본 결과, 빛이 있는 조건에서 약 3 h 주기로 10 min간 주입하는 조건이 가장 우수한 성장 및 이산화탄소 고정화를 한 것으로 나타났으며, 빛이 없을 때는 이산화탄소 주입이 무의미하다는 결론을 얻을 수 있었다.

Keywords

References

  1. S. H. Schmeider, Science, 243, 771 (1989). https://doi.org/10.1126/science.243.4892.771
  2. L. Binaghi, A. D. Borghi, A. Lodi, A. Converti, and M. D. Borghi, Proc. Biochem., 38, 1241 (2003).
  3. H. K. Park, H. J. Park, and B. S. Kang, DCER Techinfo part I, 3, 100 (2004).
  4. I. H. Lee, S. I. Kim, and J. Y. Park, Ind. Chem., 18, 239 (2007).
  5. H. D. Hwang, H. Y. Shin, H. H. Kwak, and S. Y. Bae, Korean Chem. Eng. Res., 44, 588 (2006).
  6. T. Karube, T. Takeuchi, and D. J. Barnes, Adv. Biochem. Eng. Biotechnol., 46, 63 (1992).
  7. D. O. Hall and J. I. House, Energy Convers. Manag., 34, 889 (1993). https://doi.org/10.1016/0196-8904(93)90033-7
  8. A. V. C. Jorge, A. L. Giani, I. P. A. Daniel, M. M. Guilherme, and T. K. Roselini, World J. Microbial. Biotechnol., 16, 15 (2000). https://doi.org/10.1023/A:1008992826344
  9. M. Tredici, G. C. Zitelli, S. Biagiolini, and R. Materassi, Bull. Inst. Oceanogr., 12, 89 (1993).
  10. H. M. Oh, J. S. Kim, and S. J. Lee, Kor. J. of Environ. Biol., 16, 291 (1998).
  11. T. H. Kim, K. D. Sung, J. S. Lee, J. Y. Lee, S. J. Oh, and H. Y. Lee, Kor. J. Appl. Microbiol. Biotechnol., 25, 235 (1997).
  12. S. M. Jeon, I. H. Kim, J. M. Ha, and J. H. Lee, J. Korean Ind. Eng. Chem., 19, 145 (2008).
  13. D. S. Joo, C. K. Jung, C. H. Lee, and S. Y. Cho, J. Korean Fish. Soc., 33, 475 (2000).
  14. F. Martinez-Jeronimo and F. Espinosa-Chavez, J. Appl. Phycol., 6, 423 (1994) https://doi.org/10.1007/BF02182159
  15. C. O. Rangel-Yagui, E. D. G. Danesi, J. C. M. Carvalho, and S. Sato, Bioresour. Technol., 92, 133 (2004). https://doi.org/10.1016/j.biortech.2003.09.002
  16. R. Rippka, J. Deruelles, J. B. Waterbury, M. Herdman, and P. G. Roughan, J. Sci. Food Agric., 47, 295 (1989).
  17. S. C. Babu and B. Rajasekaran, Food Policy, 9, 405 (1991).
  18. E. W. Becker and L. V. Vanattaraman, Biomass, 4, 105 (1984). https://doi.org/10.1016/0144-4565(84)90060-X
  19. O. Ciferri, Microbiol. Rev., 47, 551 (1983).
  20. L. M. Mosulishvili, E. I. Kirkesali, A. I. Belokobylsky, A. I. Khizanishvili, M. V. Frontasyeva, S. S. Pavlov, and S. F. Gundorina, J. Pharm. Biomed. Anal., 30, 87 (2002). https://doi.org/10.1016/S0731-7085(02)00199-1
  21. J. Lu, G. Yoshizaki, K. Sakai, and T. Takeuchi, Fish. Sci., 68, 51 (2002). https://doi.org/10.1046/j.1444-2906.2002.00388.x
  22. A. Vonshak, Biotechnol. Adv., 8, 709 (1990). https://doi.org/10.1016/0734-9750(90)91993-Q
  23. T. Ogawa and G. Terui, J. Ferment. Technol., 48, 361 (1970).
  24. M. G. Morais and J. A. V. Costa, J. Biotechnol., 129, 439 (2007). https://doi.org/10.1016/j.jbiotec.2007.01.009
  25. C. C. Reichert, C. O. Reinehr, and J. A. V. Costa, Braz. J. Chem. Eng., 23, 23 (2006). https://doi.org/10.1590/S0104-66322006000100003
  26. A. Vonshak, A. Abeliovich, S. Boussiba, S. Arad, and A. Richmond, Biomass, 2, 175, (1982). https://doi.org/10.1016/0144-4565(82)90028-2
  27. L. H. Pelizer, E. D. G. Danesi, C. O. Rangel, C. E. N. Sassano, J. C. M. Carvalho, S. Sato, and I. O. Moraes, J. Food Eng., 56, 371 (2003). https://doi.org/10.1016/S0260-8774(02)00209-1
  28. D. Soletto, L. Binaghi, L. Ferrari, A. Lodi, J. C. M. Carvalho, M. Zilli, and A. Converti, Biochem. Eng. J., 39, 369 (2008). https://doi.org/10.1016/j.bej.2007.10.007
  29. Y. S. Yun, J. M. Prak, and B. volesky, Kor. J. Chem. Eng., 37, 800 (1999).