Korean Journal of Chemical Engineering

The Korean Institute of Chemical Engineers (한국화학공학회)
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Optimization of medium for phenylalanine ammonia lyase production in E. coli using response surface methodology

  • Cui, Jian-Dong (Hebei Fermentation Engineering Research Center, College of Bioscience and Bioengineering, Hebei University of Science and Technology)
  • Received : 2009.01.16
  • Accepted : 2009.03.17
  • Published : 2010.01.01
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Abstract

A culture medium for phenylalanine ammonia lyase (PAL) production in E. coli was developed following preliminary studies by means of response surface methodology (RSM). The medium components having significant effect on the production were first identified by using a fractional factorial design. Then, central composite design (CCD) was used to optimize the medium constituents and explain the combined effects of four medium constituents: glucose, yeast extract, $(NH_4)_2HPO_4$ and $MgSO_4$. A quadratic model was found to fit the PAL production. CCD revealed that the optimum values of the test variables for PAL production were glucose 28.2 g/L, yeast extract 5.01 g/L, $(NH_4)_2HPO_4$ 7.02 g/L and $MgSO_4$ 1.5 g/L. PAL production of 62.85 U/g, which was in agreement with the prediction, was observed in the verification experiment. In comparison to the production of basal medium, 1.8-fold increase was obtained.

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