Journal of Korean Society of Forest Science (한국산림과학회지)

Korean Society of Forest Science (한국산림과학회)
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Effects of Storage Duration, Medium and Viscin on in vitro seed Germination in Endangered Species, Loranthus tanakae

저장기간, 배지종류 및 viscin이 멸종위기종 꼬리겨우살이의 기내 종자발아에 미치는 영향

  • Lee, Su-Gwang (Lab. of Environmental Biotechnology, Department of Biological & Environmental Science, College of Life Science and Biotechnology, Dongguk University) ;
  • Lee, Song-Hee (Lab. of Environmental Biotechnology, Department of Biological & Environmental Science, College of Life Science and Biotechnology, Dongguk University) ;
  • Kang, Ho-Duck (Lab. of Environmental Biotechnology, Department of Biological & Environmental Science, College of Life Science and Biotechnology, Dongguk University)
  • 이수광 (동국대학교 바이오시스템대학 바이오환경과학과 환경생명공학연구실) ;
  • 이송희 (동국대학교 바이오시스템대학 바이오환경과학과 환경생명공학연구실) ;
  • 강호덕 (동국대학교 바이오시스템대학 바이오환경과학과 환경생명공학연구실)
  • Received : 2010.06.14
  • Accepted : 2010.06.30
  • Published : 2010.08.30
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Abstract

This study was conducted to establish the in vitro seed germination of Loranthus tanakae. A factorial experiment evaluated the effects of seed storage duration (0, 8, 16 weeks), media (MS, SH, White, WPM), presence of viscin, GA3, gelling agent and concentrations. Seed germinated after one week culture in in vitro condition and produced radicles. In vitro seed germination was optimal when the seeds removed viscin placed on SH medium (69%) and the addition of 0.35% gelrite (75%) in the same medium. As seed storage duration was expanded to 8 or 16 weeks, in vitro seed germination rate was reduced rapidly. Holdfasts were also produced at the side of radicles. The important factors to produce holdfast and haustorium was kind of media as an optimal condition in White medium without any supplements to be shown 98% and 8% respectively. Process of in vitro germination of Loranthus tanakae was followed to radicle elongation, holdfast development and then haustorium formation sequencially.

본 연구는 멸종위기에 처한 꼬리겨우살이 종자로부터 기내배양체계 기반확립을 위해 기내 종자발아 실험을 실시하였다. 꼬리겨우살이 종자는 배지에 치상 1주 후 원기근이 형성되어 발아가 진행되었다. 배지종류와 viscin 유 무에 따른 꼬리겨우살이의 기내 종자발아율은 viscin이 제거된 종자를 SH 배지에 치상하였을 때 가장 높은 발아율(69%)을 나타냈고, 고형지지물 종류와 농도에 따른 발아율은 0.35% gelrite 첨가 시 가장 높은 발아율(75%)을 보였다. 저장 기간과 저장 상태에 따른 발아율은 채집 직후(3일 후) 조건에서 가장 높은 발아율을 나타냈고, 저장기간이 8주, 16주로 길어짐에 따라 발아율은 감소하였다. 흡착판과 흡기근은 White 기본배지에 치상하였을 때 각각 95%, 8%의 형성율을 나타내었다. 꼬리겨우살이의 기내 종자발아과정은 원기근 형성, 흡착판 형성, 흡기근 형성 순으로 진행되었다.

Keywords

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