Genes and Genomics

The Genetics Society of Korea (한국유전학회)
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Informative SSR markers for commercial variety discrimination in watermelon (Citrullus lanatus)

  • Kwon, Yong-Sham (Variety Testing Division, Korea Seed & Variety Service, MIFAFF) ;
  • Oh, You-Hwan (Variety Testing Division, Korea Seed & Variety Service, MIFAFF) ;
  • Yi, Seung-In (Plant Variety Protection Division, Korea Seed & Variety Service, MIFAFF) ;
  • Kim, Hee-Yeul (Variety Testing Division, Korea Seed & Variety Service, MIFAFF) ;
  • An, Jong-Moon (Breeding Institute, Nong Woo Bio Co.) ;
  • Yang, Seung-Gyun (Breeding Institute, Nong Woo Bio Co.) ;
  • Ok, Sung-Han (Divison of Plant Technology, Graduate School of Biotechnology, Korea University) ;
  • Shin, Jeong-Sheop (Divison of Plant Technology, Graduate School of Biotechnology, Korea University)
  • Received : 2008.12.13
  • Accepted : 2010.02.11
  • Published : 2010.04.30
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Abstract

SSR markers were used for variety discrimination and genetic assessment in watermelon varieties. Genetic characterization of 49 watermelon varieties was investigated using 30 SSR markers developed from melon and watermelon. A total of 121 polymorphic amplified fragments were obtained by using 30 SSR markers. The average polymorphism information content (PIC) was 0.502 ranging from 0.223 to 0.800. One hundred twenty one SSR loci were used to calculate Jaccard's distance coefficients for unweighted pair group method using the arithmetic averages (UPGMA) cluster analysis. A clustering group of varieties, based on the results of SSR analysis, were categorized into 5 major groups corresponding to morphological traits. Inheritance mode of 2 SSR markers was investigated to $F_1$ plants and $F_2$ populations of 2 crosses. Parental alleles were transmitted from $F_1$ plants and $F_2$ populations. Therefore, these marker sets may prove to be effectively applicable to genetic assessment of germplasm, genome mapping, and fingerprinting of watermelon varieties.

Keywords

References

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