Laboraroty Animal Research

Korean Association for Laboratory Animal Science (한국실험동물학회)
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Inhibitory Effect of Luteolin Liposome Solution by Animal Model for Atopic Dermatitis in NC/Nga Mice

  • Received : 2010.01.21
  • Accepted : 2010.03.13
  • Published : 2010.03.30
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Abstract

Atopic dermatitis is a common chronic inflammatory skin disease, associated with marked inflammatory cells (of mast cells and eosinophils) and severe itching, which leads to clinical problems in the pediatric population. This study was designed to investigate the inhibitory effects of luteolin liposome solution, that is entrapped the hydrophobic luteolin (one of the flavonoids) into ethosome to improve its stability, by using hapten-induced atopic dermatitis animal model (NC/Nga mice).The luteolin liposome treated mice showed anti-inflammatory effect as evidenced by the lowering of erythema and edema in clinical observation, reduction of inflammatory cell infiltration and epidermal thickness in histopathological examination, when compared with TNCB induced controls. Luteolin liposome solution also reduced the serum IgE level which played important roles in the atopic dermatitis model. These results suggest that luteolin liposome solution has some merit in this formulation showing inhibitory effects for the atopic dermatitis.

Keywords

References

  1. Ashokkumar, P. and Sudhandiran, G. (2008) Protective role of luteolin on the status of lipid peroxidation and antioxidant defense against azoxymethane-induced experimental colon carcinogenesis. Biomed. & Pharmacotherapy 62, 590-597. https://doi.org/10.1016/j.biopha.2008.06.031
  2. Carstens, E. and Kuraishi, Y. (2004) Animal models of itch: scratching away at the problem. In Itch Basic Mechanisms and Therapy (Yosipovitch, G. ed.), pp.35-50, Informa Healthcare, USA.
  3. Chen, C.Y., Peng, W.H., Tsai, K.D. and Hsu, S.L. (2007) Luteolin suppresses inflammation-associated gene expression by blocking NF-${\kappa}$B and AP-1 activation pathway in mouse alveolar macrophages. Life Sci. 81, 1602 - 1614. https://doi.org/10.1016/j.lfs.2007.09.028
  4. Cumberbatch, M., Clelland, K., Dearman, R.J. and Kimber, I. (2005) Impact of cutaneous IL-10 on resident epidermal Langerhans' cells and the development of polarized immune responses. J. Immunol. 175(1), 43-50.
  5. Elias, P.M. and Menon, G.X. (1991) Structural and lipid biochemical correlation of the epidermal permeability barrier. Adv. Lipid Res. 24, 1-27.
  6. Falus, A. and Merétey, K. (1992) Histamine: an early messenger in inflammatory and immune reactions. Immunol. Today 13(5), 154-156. https://doi.org/10.1016/0167-5699(92)90117-P
  7. Friedman, P.S. (2006) Contact sensitization and allergic contact dermatitis immunobiological mechanisms. Toxicol. Lett. 162, 49-54. https://doi.org/10.1016/j.toxlet.2005.10.008
  8. Furue, M., Terao, H., Moroi, Y., Koga, T., Kubota, Y., Nakayama, J., Furukawa, F., Tanaka, Y., Katayama, I., Kinukawa, N., Nose, Y. and Urabe, K. (2004) Dosage and adverse effects of topical tacrolimus and steroids in daily management of atopic dermatitis. J. Dermatol. 31(4), 277-283.
  9. Goutet, M., Pepin, E., Langonne, I., Huguet, N. and Ban, M. (2005) Identification of contact and respiratory sensitizers using flow cytometry. Toxicol. Appl. Pharmacol. 205(3), 259- 270. https://doi.org/10.1016/j.taap.2004.10.011
  10. Hruza, L.L. and Pentland, A.P. (1993) Mechanism of UV induced inflammation. J. Invest. Dermatol. 15(2), 111-115.
  11. Kaefer, C.M. and Milner, J.A. (2008) The role of herbs and spices in cancer prevention. J. Nutri. Biochem. 19, 347-361. https://doi.org/10.1016/j.jnutbio.2007.11.003
  12. Lee, S.H., Baek, S.J., Kim, H.A. and Heo, Y. (2006) 2,4- dinitrochlorobenzene-induced atopic dermatitis like immune alteration in mice. J. Toxicol. Pub. Health 22(4), 357-364.
  13. Malaviya, R., Morrison, A.R. and Pentland, A.P. (1996) Histamine in human epidermal cells is induced by ultraviolet light injury. J. Invest. Dermatol. 106(4), 785-789. https://doi.org/10.1111/1523-1747.ep12346356
  14. Matsuda, H., Watanabe, N., Geba, G.P., Sperl, J., Tsudzuki, M., Hiroi, J., Matsumoto, M., Ushio, H., Saito, S., Askenase, P.W. and Ra, C. (1997) Development of atopic dermatitis-like skin lesion with IgE hyperproduction in NC/Nga mice. Int. Immunol. 9(3), 461-466. https://doi.org/10.1093/intimm/9.3.461
  15. Matsumoto, M., Ra, C., Kawamoto, K., Sato, H., Itakura, A., Sawada, J., Ushio, H., Suto, H., Mitsuishi, K., Hikasa, Y. and Matsuda, H. (1999) IgE hyperproduction through enhanced tyrosine phosphorylation of Janus kinase 3 in NC/Nga mice, a model for human atopic dermatitis. J. Immunol. 162(2), 1056-1063.
  16. McGirt, L.Y. and Beck L.A. (2006) Innate immune defects in atopic dermatitis. J. of Allergy and Clin. Immunol. 118(1), 202-208. https://doi.org/10.1016/j.jaci.2006.04.033
  17. Menzies-Gow, A., Ying, S., Sabroe, I., Stubbs, V.L., Soler, D., Williams, T.J. and Kay, A.B. (2002) Eotaxin (CCL11) and eotaxin-2 (CCL24) induce recruitment of eosinophils, basophils, neutrophils, and macrophages as well as features of early- and late-phase allergic reactions following cutaneous injection in human atopic and nonatopic volunteers. J. Immunol. 169(5), 2712-2718.
  18. Mueller, D.L., Jenkins, M.K. and Schwartz, R.H. (1989) Clonal expansion versus functional clonal inactivation: a costimulatory signalling pathway determines the outcome of T cell antigen receptor occupancy. Annu. Rev. Immunol. 7, 445-480. https://doi.org/10.1146/annurev.iy.07.040189.002305
  19. Plitnick, L.M., Loveless, S.E., Ladics, G.S., Holsapple, M.P., Selgrade, M.J., Sailstad, D.M. and Smialowicz, R.J. (2002) Cytokine profiling for chemical sensitizers: application of the ribonuclease protection assay and effect of dose. Toxicol. Appl. Pharmacol. 179(3), 145-154. https://doi.org/10.1006/taap.2002.9370
  20. Renz, H., Brodie, C., Bradley, K., Leung, D.Y. and Gelfand, E.W. (1994) Enhancement of IgE production by anti-CD40 antibody in atopic dermatitis. J. Allergy Clin. Immunol. 93(3), 658-668. https://doi.org/10.1016/S0091-6749(94)70078-8
  21. Schuller, E., Oppel T., Bornhovd, E., Wetzel, S. and Wollenberg, A (2004) Tacrolimus ointment causes inflammatory dendritic epidermal cell depletion but no Langerhans cell apoptosis in patients with atopic dermatitis. J. Allergy and Clin. Immunol. 114(1), 137-143. https://doi.org/10.1016/j.jaci.2004.03.021
  22. Schultz-Larsen, F. and Hanifin, J.M. (2002) Epidemiology of atopic dermatitis. Immunol. Allergy Clin. North Am. 22, 1-24. https://doi.org/10.1016/S0889-8561(03)00066-3
  23. Shiohara, T., Hayakawa, J. and Mizukawa, Y. (2004) Animal models for atopic dermatitis: are they relevant to human disease? J. Dermatol. Sci. 36(1), 1-9. https://doi.org/10.1016/j.jdermsci.2004.02.013
  24. Taniguchi, Y., Kohno, K., Inoue, S., Koya-Miyata, S., Okamoto, I., Arai, N., Iwaki, K., Ikeda, M. and Kurimoto, M. (2003) Oral administration of royal jelly inhibits the development of atopic dermatitis-like skin lesions in NC/Nga mice. International Immunopharmacol. 3(9), 1313-1324. https://doi.org/10.1016/S1567-5769(03)00132-2
  25. Touitou, E., Dayan, N., Bergelson, L., Godin, B. and Eliaz, M. (2000) Ethosomes-novel vesicular carriers for enhanced delivery: characterization and skin penetration properties J. Control. Release, 65, 403-418. https://doi.org/10.1016/S0168-3659(99)00222-9
  26. Touitou, E., Meidan, V.M. and Horwitz, E. (1998) Methods for quantitative determination of drug localized in the skin. J. Control. Release, 56, 7-21. https://doi.org/10.1016/S0168-3659(98)00060-1
  27. Wisselink, M.A. and Willemse T (2009) The efficacy of cyclosporine A in cats with presumed atopic dermatitis: A double blind, randomised prednisolone-controlled study. The Vet. Journal, 180, 55-59. https://doi.org/10.1016/j.tvjl.2007.11.018
  28. Yano, S., Umeda, D., Yamashita, S., Yamada, K. and Tachibana, H. (2009) Dietary apigenin attenuates the development of atopic dermatitis-like skin lesions in NC/Nga mice. J. Nutri. Biochem. 20(11), 876-881. https://doi.org/10.1016/j.jnutbio.2008.08.002
  29. Ying, S., Robinson, D.S., Meng, Q., Barata, L.T., McEuen, A.R., Buckley, M.G., Walls, A.F., Askenase, P.W. and Kay, A.B. (1999) C-C chemokines in allergen-induced late-phase cutaneous responses in atopic subjects: association of eotaxin with early 6-hour eosinophils, and of eotaxin-2 and monocyte chemoattractant protein-4 with the later 24-hour tissue eosinophilia, and relationship to basophils and other CC chemokines (monocyte chemoattractant protein-3 and RANTES). J. Immunol. 163(7), 3976-3984.
  30. Yun, J.W., Kim, H., Kang, H.J., Koh, J.Y. and Kim, B.H. (2008): Models for Atopic Dermatitis in NC/Nga Mice. Lab. Animal Research, 24(1), 59-66.