Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Characterizing a Full Spectrum of Physico-Chemical Properties of Ginsenosides Rb1 and Rg1 to Be Proposed as Standard Reference Materials

  • Kim, Il-Woung (Department of Life Science, College of Biomedical & Health Science, Konkuk University) ;
  • Hong, Hee-Do (Korea Food Research Institute) ;
  • Choi, Sang-Yoon (Korea Food Research Institute) ;
  • Hwang, Da-Hye (Department of Life Science, College of Biomedical & Health Science, Konkuk University) ;
  • Her, Youl (Bitgen) ;
  • Kim, Si-Kwan (Department of Life Science, College of Biomedical & Health Science, Konkuk University)
  • Received : 2011.08.18
  • Accepted : 2011.11.17
  • Published : 2011.12.26
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Abstract

Good manufacturing practice (GMP)-based quality control is an integral component of the common technical document, a formal documentation process for applying a marketing authorization holder to those countries where ginseng is classified as a medicine. In addition, authentication of the physico-chemical properties of ginsenoside reference materials, and qualitative and quantitative batch analytical data based on validated analytical procedures are prerequisites for certifying GMP. Therefore, the aim of this study was to propose an authentication process for isolated ginsenosides $Rb_1$ and $Rg_1$ as reference materials (RM) and for these compounds to be designated as RMs for ginseng preparations throughout the world. Ginsenoside $Rb_1$ and $Rg_1$ were isolated by Diaion HP-20 adsorption chromatography, silica gel flash chromatography, recrystallization, and preparative HPLC. HPLC fractions corresponding to those two ginsenosides were recrystallized in appropriate solvents for the analysis of physico-chemical properties. Documentation of the isolated ginsenosides was made according to the method proposed by Gaedcke and Steinhoff. The ginsenosides were subjected to analyses of their general characteristics, identification, purity, content quantitation, and mass balance tests. The isolated ginsenosides were proven to be a single compound when analyzed by three different HPLC systems. Also, the water content was found to be 0.940% for $Rb_1$ and 0.485% for $Rg_1$, meaning that the net mass balance for ginsenoside $Rb_1$ and $Rg_1$ were 99.060% and 99.515%, respectively. From these results, we could assess and propose a full spectrum of physicochemical properties for the ginsenosides $Rb_1$ and $Rg_1$ as standard reference materials for GMP-based quality control.

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References

  1. Kwak YS, Park JD, Yang JW. Present and its prospect of red ginseng efficacy research. Food Ind Nutr 2003;8:30-37.
  2. Tanaka O. Recent studies on the chemistry of ginseng saponins. Korean J Ginseng Sci 1977;2:9-15.
  3. Han KD. The trends in chemical studies of ginseng. Korean J Ginseng Sci 1977;2:1-7.
  4. Shibata S, Tanaka O, Soma K, Ando T, Iida Y, Nakamura H. Studies on saponins and sapogenins of ginseng. The structure of panaxatriol. Tetrahedron Lett 1965;42:207-213.
  5. International Conference on Harmonization. Validation of analytical procedures: methodology. Geneva: International Conference on Harmonization, 1997.
  6. Blasius H, Gaedcke F, Steinhoff B. Herbal medicinal products: scientific and regulatory basis for development, quality assurance and marketing authorisation. Stuttgart: Medpharm Scientific Pub., 2003.
  7. Council of Europe. European pharmacopoeia. 6th ed. Strasbourg: Council of Europe, 2008.
  8. Dong A, Ye M, Guo H, Zheng J, Guo D. Microbial transformation of ginsenoside $Rb_1$ by Rhizopus stolonifer and Curvularia lunata. Biotechnol Lett 2003;25:339-344. https://doi.org/10.1023/A:1022320824000
  9. Teng R, Li H, Chen J, Wang D, He Y, Yang C. Complete assignment of $^1H$ and $^{13}C$ NMR data for nine protopanaxatriol glycosides. Magn Reson Chem 2002;40:483-488. https://doi.org/10.1002/mrc.1033
  10. Wang W, Zhao Y, Rayburn ER, Hill DL, Wang H, Zhang R. In vitro anti-cancer activity and structure-activity relationships of natural products isolated from fruits of Panax ginseng. Cancer Chemother Pharmacol 2007;59:589-601. https://doi.org/10.1007/s00280-006-0300-z
  11. Cho JG, Lee MK, Lee JW, Park HJ, Lee DY, Lee YH, Yang DC, Baek NI. Physicochemical characterization and NMR assignments of ginsenosides $Rb_1$, $Rb_2$, Rc, and Rd isolated from Panax ginseng. J Ginseng Res 2010;34;113-121. https://doi.org/10.5142/jgr.2010.34.2.113
  12. Shao C, Xu D, Kasai R, Tanaka O. Saponins from flower-buds of Panax ginseng cultivated at Jilin, China. Chem Pharm Bull 1989;37:1934-1935. https://doi.org/10.1248/cpb.37.1934
  13. Su J, Li HZ, Yang CR. Studies on saponin constituents in roots of Panax quinquefolium. Zhongguo Zhong Yao Za Zhi 2003;28:830-833.
  14. Kwak YS, Kyung JS, Kim SK, Wee JJ. An isolation of crude saponin from red-ginseng efflux by Diaion HP-20 resin adsorption methd. J Korean Soc Food Sci Nutr 2001;30:1-5.
  15. Kim DH, Chang JK, Sohn HJ, Cho BG, Ko SR, Nho KB, Jang DS, Lee SM. Certification of a pure reference material for the ginsenoside $Rg_1$. Accred Qual Assur 2010;15:81-87. https://doi.org/10.1007/s00769-009-0588-6

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