Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Development of In Vitro Seedling Screening Method for Selection of Resistant Rice Against Bakanae Disease

벼 키다리병 저항성 검정을 위한 기내 유묘 검정법 개발

  • Lee, Yong-Hwan (Knowledge and Information Officer Division, Rural Development Administration) ;
  • Lee, Myeong-Ji (Department of Agricultural Biology, National Academy of Agricultural Science, Development Administration) ;
  • Choi, Hyo-Won (Department of Agricultural Biology, National Academy of Agricultural Science, Development Administration) ;
  • Kim, Sung-Taek (Department of Agricultural Biology, National Academy of Agricultural Science, Development Administration) ;
  • Park, Jin-Woo (Department of Agricultural Biology, National Academy of Agricultural Science, Development Administration) ;
  • Myung, Inn-Shik (Department of Agricultural Biology, National Academy of Agricultural Science, Development Administration) ;
  • Park, Kyung-Seok (Department of Agricultural Biology, National Academy of Agricultural Science, Development Administration) ;
  • Lee, Se-Weon (Department of Agricultural Biology, National Academy of Agricultural Science, Development Administration)
  • 이용환 (농촌진흥청 기획조정관실) ;
  • 이명지 (국립농업과학원 농업생물부) ;
  • 최효원 (국립농업과학원 농업생물부) ;
  • 김성택 (국립농업과학원 농업생물부) ;
  • 박진우 (국립농업과학원 농업생물부) ;
  • 명인식 (국립농업과학원 농업생물부) ;
  • 박경석 (국립농업과학원 농업생물부) ;
  • 이세원 (국립농업과학원 농업생물부)
  • Received : 2011.11.16
  • Accepted : 2011.11.28
  • Published : 2011.12.31
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Abstract

Rice bakanae disease, caused by the fungus Fusarium fujikuroi, is one of the most important rice diseases and distributed widely in Asia. Resistance screening system in rice field had been established. However, the evaluation results of the system vary according to the environmental conditions when the test is conducted. To develop precise and rapid evaluation method of disease resistance of rice to bakanae disease, in vitro screening system was attempted in this study. The six cultivars namely, 'Nampyeongbyeo', 'Junambyeo', 'Chucheongbyeo', 'Samcheonbyeo', 'Odaebyeo' and 'Hwasinbyeo' were tested. They were planted onto MS agar medium (10 ml) in test tube ($450\;mm{\times}{\phi}30\;mm$) and incubated at $25^{\circ}C$ and $28^{\circ}C$ in growth chamber under 12 hr light condition. Symptoms of over growth appeared a few days after seeding and then seedling were withered 2-3 weeks after over growth. The disease symptoms such as leaf dryness on top of rice were appeared in the 'Nampyeongbyeo' from 28 days at the concentration of $10^5$ spores/ml culturing at $28^{\circ}C$ and then withered completely 35 days after seeding. Whereas the other varieties withered entirely 19-23 days after seeding. Using the in vitro seedling screening method, 72 rice varieties were investigated to select resistant cultivar. Finally, two resistant cultivars ('Nampyeongbyeo' and 'Inwolbyeo') and seven moderately resistant cultivars ('Hwadongbyeo', 'Seokjeongbyeo', 'Samgwangbyeo', 'Sampyeongbyeo', 'Nonghobyeo', 'Heukjinjubyeo' and, 'Joanbyeo') were selected. If in vitro seedling screening method was used for evaluation of bakanae disease resistance, it would be completed within 35 days after sowing of rice seed.

벼 키다리병은 매우 중요한 벼의 병 가운데 하나로 아시아 지역에서 넓게 발생하고 있다. 포장에서의 저항성 검정체계가 개발되어 있지만, 그 검정 결과가 환경조건에 따라 다르게 나타나는 문제점을 가지고 있다. 본 연구에서는 정확하고 빠른 키다리병 품종 저항성 검정을 위하여 기내에서 검정하는 시스템을 개발하였다. 병원균을 접종한 '남평벼', '주남벼', '추청벼', '삼천벼', '오대벼', '화신벼' 등 6개 품종의 종자를 MS배지가 10 ml 씩 담겨 있는 직경 30 mm, 길이 450 mm 시험관에 벼 종자 3립씩 파종 후 $25^{\circ}C$와 $28^{\circ}C$ 항온기에 치상하고 하루 12시간 광조건으로 배양하였다. 키다리병 증상은 벼가 도장한 후에 2-3주 경과시점 부터 잎 끝이 마르기 시작하여 고사하게 된다. $10^5$ 포자/ml 농도와 $28^{\circ}C$ 조건에서 '남평벼'는 파종후 28일째부터 잎 끝이 마르는 증상을 보이기 시작하여 35일째에 완전히 고사를 하는 반면 다른 품종들은 19-23일째에 모두 고사하였다. 기내유묘검정법을 이용하여 72개 품종에 대해 저항성검정을 한 결과 '남평벼'와 '인월벼'를 저항성으로 '화동벼', '석정벼', '삼광벼', '삼평벼', '농호벼', '흑진주벼', '조안벼'를 중도저항성으로 선발하였다. 기내유묘검정법을 이용할 경우 파종 후 35일 이내에 키다리병 품종저항성 검정을 완료할 수 있었다.

Keywords

References

  1. Amoah, B. K., MacDonald, M. V., Rezanoor, H. N. and Nicholson, P. 1996. The use of random amplified polymorphic DNA technique to identify mating groups in the Fusarium Section Liseola. Plant Pathol. 45: 115-125. https://doi.org/10.1046/j.1365-3059.1996.d01-95.x
  2. Amoah, B. K., Rezanoor, H. N., Nicholson, P. and MacDonald, M. V. 1995. Variation in the Fusarium Section Liseola: pathogenicity and genetic studies of isolates of Fusarium moniliformae Sheldon from different hosts in Ghana. Plant Pathol. 44: 563-572. https://doi.org/10.1111/j.1365-3059.1995.tb01678.x
  3. Booth, C. 1971. The genus Fusarium. Commonwealth Mycological Institute, Kew, Surrey, 221 pp.
  4. Desjardins, A. E., Plattner, R. D. and Nelson, P. E. 1997. Production of fumonisin B sub 1 and moniliformin by Gibberella fujikuroi from rice from various geographic areas. Appl. Environ. Microbiol. 63: 1838-1842.
  5. Gill, M. A., Yasin, S. I., Khan, T. Z., Ahsanullah, Q. and Butt, M. A. 1993. Evaluation of resistance to bakanae and foot rot disease. Int. Rice Res. Notes 18: 14-15.
  6. Han, S. 2007. Review of disease occurrence of major crops in Korea in 2007. Proc. Annu. Fall Meeting Symposium KSPP. pp. 19-20.
  7. Haque, M. A., Rahman, M. M. and Miah, S. A. 1979. A rapid method of screening rice varieties for resistance to bakanae disease in Bangladesh. Int. Rice Commission Newsletter (FAO) 28: 34-37.
  8. Hashioka, Y. 1952. Varietal resistance of rice to the 'Bakanae' disease. (Studies on pathological breeding of rice V.) Jpn. J. Breeding 1: 167-171. https://doi.org/10.1270/jsbbs1951.1.167
  9. Ito, S. and Kimura, J. 1931. Studies on Bakanae disease of the rice plant. Hokkaido Agri. Exp. Stn. 27: 1-95.
  10. Katayama, T. 1951. Studies on tillering of rice and barley (Ine.mugi no bungestsu kenkyu). Yokendo, Tokyo.
  11. Khokhar, L. K. 2002. Identification of sourcese of resistance against bakanae and foot rot disease in rice. Pakistan J. Agric. Res. 17: 176-177.
  12. Kim, C. 1981. Ecological studies of bakanae disease of rice, caused by Gibberella fujikuroi. Korean J. Plant Prot. 20: 146-151.
  13. Korea Crop Protection Association. 2011. Guideline for pesticide usage. pp. 288-292, 421.
  14. Lee, Y. H., Kim, S. Y., Choi H.-W., Lee, M.-J., Ra, D. S., Kim, I. S., Park, J. W. and Lee, S.-W. 2010. Fungicide resistance of Fusarium fujikuroi isolates isolated in Korea. Korean J. Pest Sci. 14: 427−432.
  15. Mohd Zainudin, N. A. I., Razak, A. A. and Salleh, B. 2008. Bakanae disease of rice in Malaysia and Indonesia: Etiology of the causal agent based on morphological, physiological and pathogenicity characteristics. J. Plant Prot. Res. 48: 475-485. https://doi.org/10.2478/v10045-008-0056-z
  16. Nirenberg, H. I. 1976. Untersuchungen uber die morphologische and biologische differenzierung in Fusarium-Sektion Liseola. Mitt. Biol. Vundesansi. Land-Forstwirtsch. Berlin-Dahlem 169: 107-117.
  17. O'Donnell, K., Cigelnik, E. and Nirenberg, H. 1998. Molecular systematics and phylogeography of the Gibberella fujikuroi species complex. Mycologia 90: 465-493. https://doi.org/10.2307/3761407
  18. Ou, S. H. 1985. Rice disease, 2nd ed. Commonwealth Mycological Institute, Kew, Surrey, pp. 380.
  19. Park, W.-S., Choi, H.-W., Han, S.-S., Shin, D.-B., Shim, H.-K., Jung, E.-S., Lee, S.-W., Lim, C.-K. and Lee, Y. H. 2009. Control of Bakanae disease of rice by seed soaking into the mixed solution of procholraz and fludioxnil. Res. Plant Dis. 15: 94-100. (In Korean) https://doi.org/10.5423/RPD.2009.15.2.094
  20. Park, W.-S., Yeh, Y.-H., Lee, S.-W., Han, S.-S., Lee, J.-S., Lim, C.-K. and Lee, Y. H. 2008. Electron microscopic study for the influence of soaking in hot water and prochloraz solution on spore and mycelium of Fusarium fujikuroi infected in rice seed. Res. Plant Dis. 14: 176-181. (In Korean) https://doi.org/10.5423/RPD.2008.14.3.176
  21. Rajagopalan, K. 1961. Screening of rice varieties for resistance to foot-rot disease. Curr. Science 30: 145-147.
  22. Reyes, G. M. 1939. Rice disease and methods of control. Phillippine J. Agric. 10: 419-436.
  23. Snyder, W. C. and Hansen, H. N. 1945. The species concept in Fusarium with reference to Discolor and other sections. Am. J. Bot. 32: 657-666. https://doi.org/10.2307/2437621
  24. Sung, J. M., Yang, S. S. and Lee, E. J. 1984. Pathogenicity in nursery box and symptom appearance and yield damage in paddy field by each strain of Fusarium moniliforme. Korean J. Mycol. 12: 65−73.
  25. Thomas, K. M. 1933. The 'foot rot' of paddy and its control. Madras Agric. J. 21: 263-272.
  26. Yamanaka, S. and Honkura, R. 1978. Symptoms on rice seedlings inoculated with "Bakanae" disease fungus, Fusarium moniliforme Sheldon. Ann. Phytopathol. Soc. Jpn. 44: 57-58. https://doi.org/10.3186/jjphytopath.44.57
  27. Yeh, W.-H., Choi, H.-W., Kim, S.-A., Shim, H.-S. and Kim, Y.-K. 2005. Studies on ecology and control of rice bakanae disease. Agricultural Biology Research, NIAST, Korea. pp. 490-508. (In Korean)

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