Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Suppression Effect and Mechanism of Citrus Scab in the Citrus Pre-inoculated with Rhizobacterial Strains

근권세균을 전 접종한 감귤에서 감귤 더뎅이병 억제 효과 및 기작

  • Kim, So-Yeon (Plant Resources and Environment, College of Applied Life Sciences, The Research Institute for Subtropical Agriculture and Biotechnology, Jeju National University) ;
  • Hyun, Jae-Wook (Citrus Research Station, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Jeun, Yong-Chull (Plant Resources and Environment, College of Applied Life Sciences, The Research Institute for Subtropical Agriculture and Biotechnology, Jeju National University)
  • 김소연 (제주대학교 생물산업학부 식물자원환경) ;
  • 현재욱 (국립원예특작과학원 감귤시험장) ;
  • 전용철 (제주대학교 생물산업학부 식물자원환경)
  • Received : 2011.08.01
  • Accepted : 2011.12.08
  • Published : 2011.12.31
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Abstract

Elsinoe fawcettii is one of major pathogenic fungi which cause citrus scab diseases, resulting in fruit blemishes that reduce the economic value of fruit. By increasing interest to safe products of crops, the alternative methods of disease control is highly required. We investigated whether the 215 bacterial strains isolated from Jeju Island possess antifungal effect or suppression effect on the symptom development by Elsinoe fawcettii on citrus. Among them, three bacterial strains THJ 609-3, MRL408-3, and TRH423-3 that exhibited antifungal capacity against Elsinoe fawcettii were selected. To illustrate the disease suppression mechanism, pre-inoculation with the selected bacterial strains was carried out whether could suppress the citrus crab on the leaves. The observation with a fluorescence microscope revealed that the selected bacteria could decrease the number of fungal spores. The ratio of germ tube formation was also decreased by the selected bacterial strains at one day after fungus challenge. The strain THJ 609-3 was identified as Pseudomonas putida as a result of analyzing the internal transcript spaces of the rhizobacterial rDNA. The strains MRL 408-3 and TRH 423-3 were identified as Burkholderia gladioli. Our results may be valuable when the selected rhizobacterial strains used as the environment-friendly microbe for biological control on citrus scab caused by Elsinoe fawcettii.

Elsinoe fawcettii는 현재 감귤 산업의 경제 가치를 떨어뜨리며, 감귤 더뎅이병을 일으키는 원인중 하나로 일반적으로 살균제를 이용하여 방제가 이루어지고 있다. 하지만 안전한 농산물에 대한 관심이 증대되면서 생물학적인 방제에 대한 연구가 많이 요구되고 있다. 이에 미생물 방제에 대한 연구로서 제주에서 분리한 식물근권세균 215개 중 Elsinoe fawcettii에 의해 발병되는 감귤 더뎅이병균의 항균 효과와 병원균 발병 억제효과가 나타나는지 조사하였다. 그 중 in vitro 실험에서 더뎅이병균에 저지원을 형성하며 항균 효과를 나타내는 근권세균은 THJ 609-3, MRL 408-3, TRH 423-3이었다. 또한 이들 식물근권세균을 선처리하고 병원균을 접종한 결과 감귤 더뎅이병 억제효과가 있었다. 근권세균에 의한 병억제 기작을 알아보기 위해 형광현미경을 이용하여 조사한 결과 선발된 식물근권 세균이 병원균의 포자수를 감소시켰으며 병원균 발아율도 접종 1일 후에 감소되었다. 식물근권세균 rDNA의 Internal Transcript Spaces(ITS)을 분석을 통해 동정한 결과 THJ 609-3은 Pseudomonas pudia로 동정되었으며, MRL 408-3과 TRH 423-3은 Burkholderia gladioli로 동정되었다. 본 연구는 생물적 방제로 감귤 더뎅이병균을 방제할 수 있는 친환경적 미생물제제로 유용하게 이용할 수 있는 가치가 있다고 생각된다.

Keywords

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