Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Analysis and Survey for Contamination of Deoxynivalenol and Zearalenone in Feed by High Performance Liquid Chromatography

HPLC를 이용한 사료 중 Deoxynivalenol, Zearalenone의 분석과 오염도 조사

  • Kim, Dong-Ho (National Agricultural Products Quality Management Service) ;
  • Choi, Kyu-Il (National Agricultural Products Quality Management Service) ;
  • Hong, Kyung-Suk (National Agricultural Products Quality Management Service) ;
  • Kim, Hyun-Jung (National Agricultural Products Quality Management Service) ;
  • Song, Yeong-Jin (National Agricultural Products Quality Management Service) ;
  • Gang, Seung-Hun (National Agricultural Products Quality Management Service) ;
  • Jang, Han-Sub (National Agricultural Products Quality Management Service) ;
  • Cho, Hyun-Jung (National Agricultural Products Quality Management Service) ;
  • Han, Gye-Su (National Agricultural Products Quality Management Service)
  • 김동호 (국립농산물품질관리원 시험연구소) ;
  • 최규일 (국립농산물품질관리원 시험연구소) ;
  • 홍경숙 (국립농산물품질관리원 시험연구소) ;
  • 김현정 (국립농산물품질관리원 시험연구소) ;
  • 송영진 (국립농산물품질관리원 시험연구소) ;
  • 강승훈 (국립농산물품질관리원 시험연구소) ;
  • 장한섭 (국립농산물품질관리원 시험연구소) ;
  • 조현정 (국립농산물품질관리원 시험연구소) ;
  • 한계수 (국립농산물품질관리원 시험연구소)
  • Received : 2011.05.17
  • Accepted : 2011.07.09
  • Published : 2011.09.30
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Abstract

Deoxynivalenol (DON) and zearalenone (ZEN) are mainly contaminated mycotoxins in feeds. The study was carried out to analyze and survey the contamination of DON and ZEN in one hundred thirteen samples of feeds. After cleaning all samples with immunoaffinity column, the mycotoxins were analysed by using high performance liquid chromatography/fluorescence with diode array detector (HPLC/FLD with DAD). The average recoveries of DON were 88.76 and 95.40% at the levels of 200 and 1,000 ${\mu}g/kg$ and 87.09 and 98.40% of ZEN were recovered at the levels of 100 and 500 ${\mu}g/kg$, respectively. The limit of detection (LOD) were 6.0 and 3.0 ${\mu}g/kg$ for DON and ZEN, respectively. The average concentrations of DON were 372.1, 324.0 and 990.9 ${\mu}g/kg$ in chicken, pig and cattle feed, respectively. Those of ZEN were 76.1, 43.7 and 196.2 ${\mu}g/kg$ for them, individually.

2010년 유통사료 113점에 대하여 곰팡이독소 오염도 조사를 실시하였다. 면역친화컬럼(IAC)을 이용하여 각각 정제하였으며, HPLC/DAD와 FLD를 연결하여 동시분석하였다. DON은 200과 1,000 ${\mu}g/kg$수준에서 각각 95.40과 88.76%의 회수율과 4.03과 1.40%의 상대표준편차(%RSD)를 나타내었다. ZEN은 100과 500 ${\mu}g/kg$ 수준에서 각각 87.09과 98.40%의 회수율과 9.01과 2.81%의 상대표준편차(%RSD)를 나타내었다. 배합사료인 닭사료, 돼지사료, 소사료에서 DON과 ZEN이 각각 372.1, 324.0, 990.9 ${\mu}g/kg$와 76.1, 43.7, 196.2 ${\mu}g/kg$의 평균오염도를 보여 2009년도 조사결과보다 높은 오염수준을 나타내었다. 옥수수, 단백피, 주정박, 옥수수배아박, 옥수수글루텐에서는 DON과 ZEN이 각각 224.6, 3150.4, 721.5, 37.8, 207.3 ${\mu}g/kg$와 77.8, 308.5, 163.1, 116.9, 510.8 ${\mu}g/kg$로 나타남에 따라 역시 배합사료에 곰팡이독소를 오염시키는 주 원인물질로 확인되었다.

Keywords

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