Journal of Biomedical and Translational Research

Research Institute of Veterinary Medicine Chungbuk National University (충북대학교 동물의학연구소)
권호 표지

Teratogenic Effects of Nano- and Micro-sized Particles of Zinc Oxide during Mouse Organogenesis

Yon, Jung-Min;Jung, A-Young;Lin, Chun-Mei;Lee, Jong-Geol;Jung, Ki-Youn;Na, Han-Sung;Chung, Myeon-Woo;Lee, Beom-Jun;Yun, Young-Won;Nam, Sang-Yoon

  • Published : 20110600
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Abstract

Zinc oxide nanoparticles (nZnO) are used in a various range including ceramic manufacture, photocatalysis, UV filters, and food industry. However, very little is known about effects of micro- and nano-particle during mouse embryo organogenesis. To determine whether ZnO affects size-dependent anomalies during embryonic organogenesis, mouse embryos were cultured for 2 days with 300 ug/ml micro ZnO (mZnO; $80{\pm}25{\mu}m$) and nZnO (< 100 nm) and then the developmental changes were investigated, quantity of Zn by inductively coupled plasma mass spectrometry analysis, and expression patterns of various antioxidant enzymes in the embryos were investigated. Embryos exposed to mZnO or nZnO exhibited severe growth and development retardation. In the embryos exposed to mZnO and nZnO, yolk sac diameter, crown-rump length, and head length were significantly diminished. The morphological parameters including yolk sac circulation, allantois, flexion, heart, hindbrain, midbrain, forebrain, otic system, optic system, branchial bars, maxillary process, mandibular process, olfactory system, caudal neural tube, forelimb, hindlimb, and somites in mZnO and nZnO-treated groups were significantly decreased. Zn absorption of nZnO-treated group was significantly higher than that of mZnO-treated group. CuZn-SOD, Mn-SOD, cGPx and PHGPx mRNA levels were significantly decreased in ZnO-treated group. In addition, antioxidant enzymes mRNA expressions of nZnO group were siginificantly diminished less than those of the mZnO treated group. These findings indicate that 300 ug/ml ZnO showed abnormality and nZnO may have more severe effect than mZnO in developing embryos.

Keywords

References

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