Journal of Cancer Prevention

Korean Society of Cancer Prevention (대한암예방학회)
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Quercetin Induces Apoptosis via Regulation of mTOR-VASP Signaling Pathway in HT-29 Colon Cancer Cells

HT-29 대장암 세포에서 mTOR-VASP 신호경로 조절을 통한 쿼세틴의 Apoptosis 효과

  • Lee, Se-Hee (Department of Biological Sciences, College of Life Science and Nano Technology, Hannam University) ;
  • Kim, In-Seop (Department of Biological Sciences, College of Life Science and Nano Technology, Hannam University) ;
  • Park, Song-Yi (Department of Biological Sciences, College of Life Science and Nano Technology, Hannam University) ;
  • Park, Ock-Jin (Department of Food and Nutrition, College of Life Science and Nano Technology, Hannam University) ;
  • Kim, Young-Min (Department of Biological Sciences, College of Life Science and Nano Technology, Hannam University)
  • 이세희 (한남대학교 생명나노과학대학 생명과학과) ;
  • 김인섭 (한남대학교 생명나노과학대학 생명과학과) ;
  • 박송이 (한남대학교 생명나노과학대학 생명과학과) ;
  • 박옥진 (한남대학교 생명나노과학대학 식품영양학과) ;
  • 김영민 (한남대학교 생명나노과학대학 생명과학과)
  • Published : 2011.12.30
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Abstract

Quercetin, one kind of phytochemical, is known to an important anti-cancer flavonoid. In this study, we suggest that quercetin-treated HT-29 colon cancer cells inhibited cell proliferation and induced apoptosis through AMPK, mTOR, VASP and COX-2 signaling pathway. AMP-activated protein kinase (AMPK) acts as an intracellular energy sensor and activated by depletion status of ATP. AMPK has been recognized as an important up-regulation signaling mediator involved in the down-regulation of the mTOR and VASP pathway. Mammalian target of rapamycin (mTOR) plays a central role in regulating of cell proliferation. mTOR signaling pathway is immoderately activated in many type of cancer. We determined with MTT assay, Western blotting and FACS. Treatment of Compound C (AMPK inhibitor) resulted in increased cell proliferation of cancer cells and showed that mTOR, COX-2 and VASP are regulated by AMPK. But, COX-2, VASP are directly controlled by quercetin in AMPK-independent pathway. Also, treatment of rapamycin (mTOR inhibitor), celecoxib (COX-2 inhibitor) and co-treatment with quercetin resulted in greater decrease of cell proliferation in colon cancer cells. In addition, mTOR can modulate the expression of COX-2, and rapamycin functions as an activator of AMPK. The results indicate that quercetin decreases cell proliferation and induces apoptosis by inhibiting mTOR and COX-2. The inhibition of mTOR (or rapamycin) can control the expression of VASP, AMPK.

Keywords

References

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