Korean Journal of Chemical Engineering

The Korean Institute of Chemical Engineers (한국화학공학회)
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Constitutive overexpression of Pseudoalteromonas carrageenovora arylsulfatase in E. coli fed-batch culture

Kim, Mi-Jin;Kim, Jeong-Hwan;Nam, Soo-Wan

  • Published : 20110400
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Abstract

The arylsulfatase gene (astA) from Pseudoalteromonas carrageenovora genome was subcloned into pHCE-IA vector, in which the hyper constitutive expression (HCE) promoter from the D-amino acid aminotransferase (D-AAT) gene of Geobacillus toebii was employed. When the constructed pHCE-AST was introduced into E. coli, the transformant showed the hydrolyzing activity for 4-methylumbelliferyl-sulfate and p-nitrophenyl-sulfate. When the cell was cultured on fermentor containing MaxyBroth-HD medium with 1% glycerol, the enzyme activity reached 12.8 unit/mL. On MaxyBroth-HD medium with 2% glycerol, the cell showed 2.7-fold higher arylsulfatase expression than that with 1% glycerol. The fed-batch cultivation employing MaxyBroth-HD medium and additional feeding of glycerol gave about 143 unit/mL of arylsulfatase at 20 h, which corresponds to 4-fold higher enzyme activity than that of 2% glycerol batch culture. Most of arylsulfatase activity in fed-batch culture was produced in the extracellular medium, whereas the activity in the batch cultures was localized in the periplasmic cell space.

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