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Antioxidative Activity of Carotenoids in Mideodeok Styela clava

  • Nacional, Loda M. (College of Aquatic Sciences and Applied Technology, Mariano Marcos State University) ;
  • Kang, Seok-Joong (Deptartment of Marine Life Science/Institute of Marine Industry, Gyeongsang National University) ;
  • Choi, Byeong-Dae (Department of Seafood Science and Technology/Institute of Marine Industry, Gyeongsang National University)
  • Received : 2001.08.13
  • Accepted : 2011.11.07
  • Published : 2011.12.31

Abstract

Carotenoids were found in high levels in both muscle and tunic samples, with the highest and lowest values observed in March and January, respectively. The average values in muscle (GM) and tunic (GT) harvested in Geoje were 49.1 mg/100g and 56.7 mg/100g, respectively, whereas those in muscle (TM) and tunic (TT) harvested in Tongyeong were 42.0 mg/100g and 50.2 mg/100g, respectively. The total phenol contents of the tunic were not significantly different (P < 0.05) between sampling area and month. We investigated the antioxidative activities of the carotenoids against linoleic acid peroxidation [1,1-diphenyl-2-picrylhydrazyl (DPPH)] and hydroxyl radicals as well as their reducing power. The DPPH radical scavenging activity was 7.6-13.5% in GM, which is relatively weak, whereas it was 21.1-29.9% in GT, 9.6-12.4% in TM and 19.3-24.1% in TT. In comparison to ${\alpha}$-tocopherol, the carotenoids were found to have strong inhibitory effects against linoleic acid peroxidation, and exhibited strong hydroxyl radical scavenging activities and reducing power at 120 ${\mu}g/mL$ of each sample.

Keywords

References

  1. Aruoma OI. 1998. Free radicals, oxidative stress, and antioxidants in human health and disease. J Am Oil Chem Soc 75, 199-212. https://doi.org/10.1007/s11746-998-0032-9
  2. Chew BP and Park JS. 2004. Carotenoid action on the immune response. J Nutr 134, 257S-261S.
  3. Choi BD, Kang SJ, Choi YJ, Youm MG and Lee KH. 1994. Utilization of ascidian (Halocynthia roretzi) tunic. 3. Carotenoid composition of ascidian tunic. Bull Korean Fish Soc 27, 344-350.
  4. Chung SK, Osawa T and Kawakishi S. 1977. Hydroxyl radical scavenging effects of spices and scavengers from brown mustard (Brassica nigra). Biosci Biotechnol Biochem 61, 118-123. https://doi.org/10.1271/bbb.61.118
  5. Duh PD. 1998. Antioxidant activity of burdock (Arctium lappa Linne): its scavenging effect on free-radical and active oxygen. J Am Oil Chem Soc 75, 455-461. https://doi.org/10.1007/s11746-998-0248-8
  6. Duval B, Shetty K and Thomas WH. 2000. Phenolic compounds and antioxidant properties in the snow alga Chlamydomonas nivalis after exposure to UV light. J Appl Phycol 11, 559-566. https://doi.org/10.1023/A:1008178208949
  7. Ganesan P, Kumar CS and Bhaskar N. 2008. Antioxidant properties of methanol extract and its solvent fractions obtained from selected Indian red seaweeds. Bioresour Technol 99, 2717-2723. https://doi.org/10.1016/j.biortech.2007.07.005
  8. Hosokawa M, Okada T, Mikami N, Konishi I and Miyashita K. 2009. Bio-functions of marine carotenoids. Food Sci Biotechnol 18, 1-11.
  9. Jackson H, Braun CL and Ernst H. 2008. The chemistry of novel xanthophyll carotenoids. Am J Cardiol 101, 50D-57D. https://doi.org/10.1016/j.amjcard.2008.02.008
  10. Je JY, Ahn CB, Oh MJ and Kang SY. 2009. Antioxidant activity of a red seaweed Polysiphonia morrowii extract. Food Sci Biotechnol 18, 124-129.
  11. Karakoltsidis PA, Zotos A and Constantinides SM. 1995. Composition of the commercially important Mediterranean finfish, crustaceans, and molluscs. J Food Compost Anal 8, 258-273. https://doi.org/10.1006/jfca.1995.1019
  12. Kelly KL, Cooper EL and Raftos DA. 1993. A humoral opsonin from the solitary urochordate Styela clava. Dev Comp Immunol 17, 29-39. https://doi.org/10.1016/0145-305X(93)90013-G
  13. Krinsky NI. 1993. Actions of carotenoids in biological systems. Annu Rev Nutri 13, 561-587. https://doi.org/10.1146/annurev.nu.13.070193.003021
  14. Lee DW, You DH, Yang EK, Jang IC, Bae MS, Jeon YJ, Kim SJ and Lee SC. 2010. Antioxidant and ACE inhibitory activities of Styela clava according to harvesting time. J Korean Soc Food Sci Nutr 39, 331-336. https://doi.org/10.3746/jkfn.2010.39.3.331
  15. Matsuno T, Ookubo M and Komori T. 1985. Carotenoids of tunicates. III. The structural elucidation of two new marine carotenoids, amarouciaxanthin A and B. J Nat Prod 48, 606-613. https://doi.org/10.1021/np50040a015
  16. McBeth JW. 1972. Carotenoids from nudibranchs. Comp Biochem Physiol B Biochem Mol Biol 41, 55-68. https://doi.org/10.1016/0305-0491(72)90007-7
  17. Meir S, Kanner J, Akiri B and Philosoph-Hadas S. 1995. Determination and involvement of aqueous reducing compounds in oxidative defense systems of various senescing leaves. J Agric Food Chem 43, 1813-1819. https://doi.org/10.1021/jf00055a012
  18. Nacional LM, Lee JS, Kang SJ and Choi BD. 2006. Seasonal variation in the nutritional content of mideodeok Styela clava. J Fish Sci Technol 9, 49-56.
  19. National Fisheries Research Development Institute. 2008. Korea marine environment data [Internet]. National Fisheries Research Development Institute, Busan, KR, [Accessed 13 Aug 2011], http://www.nfrda.re.kr.
  20. Nishibori K. 1958. Studies on the pigments of marine animals. VI. Carotenoids of some tunicates. Publ Seto Mar Biol Lab Kyoto Univ Jpn 7, 181-192.
  21. Orban E, Lena GD, Nevigato T, Casini I, Marzetti A and Caproni R. 2002. Seasonal changes in meat content, condition index and chemical composition of mussels (Mytilus galloprovincialis) cultured in two different Italian sites. Food Chem 77, 57-65. https://doi.org/10.1016/S0308-8146(01)00322-3
  22. Osawa T and Namiki M. 1981. A novel type of antioxidant isolated from leaf wax of Eucalyptus leaves. Agric Biol Chem 45, 735-739. https://doi.org/10.1271/bbb1961.45.735
  23. Oyaizu M. 1986. Studies on browning reaction: antioxidative activities of browning reaction products prepared from glucose amine. Jpn J Nutr 44, 307-315. https://doi.org/10.5264/eiyogakuzashi.44.307
  24. Prochazkova D, Bousova I and Wilhelmova N. 2011. Antioxidant and prooxidant properties of flavonoids. Fitoterapia 82, 513-523. https://doi.org/10.1016/j.fitote.2011.01.018
  25. Rebachuk NM, Maksimov OB, Boguslavskaya LS and Fedoreev SA. 1985. Carotenoids of the ascidian Halocynthia aurantium. Chem Nat Compd 20, 407-409. https://doi.org/10.1007/BF00574323
  26. Rice-Evans CA, Miller NJ, Bolwell PG, Bramley PM and Pridham JB. 1995. The relative antioxidant activities of plant-derived polyphenolic flavonoids. Free Radic Res 22, 375-383. https://doi.org/10.3109/10715769509145649
  27. Roginsky V and Lissi EA. 2005. Review of methods to determine chain-breaking antioxidant activity in food. Food Chem 92, 235-254. https://doi.org/10.1016/j.foodchem.2004.08.004
  28. Sanina NM and Kostetsky EY. 2002. Thermotropic behavior of major phospholipids from marine invertebrates: changes with warm-acclimation and seasonal acclimatization. Comp Biochem Physiol B Biochem Mol Biol 133, 143-153. https://doi.org/10.1016/S1096-4959(02)00092-1
  29. Shim YY, Kakuda Y and Shi J. 2009. Synergistic antioxidant effects of lycopene and other antioxidants on methyl linoleate autooxidation. Food Sci Biotechnol 18, 904-909.
  30. Shon MY, Kim TH and Sung NJ. 2003. Antioxidants and free radical scavenging activity of Phellinus baumii (Phellinus of Hymenochaetaceae) extracts. Food Chem 82, 593-597. https://doi.org/10.1016/S0308-8146(03)00015-3
  31. Siriwardhana N, Lee K-W, Kim S-H, Ha JW and Jeon Y-J. 2003. Antioxidant activity of Hizikia fusiformis on reactive oxygen species scavenging and lipid peroxidation inhibition. Food Sci Technol Int 9, 339-346. https://doi.org/10.1177/1082013203039014
  32. Slinkard K and Singleton VL. 1977. Total phenol analysis: automation and comparison with manual methods. Am J Enol Vitic 28, 49-55.
  33. Thiyagarajan V and Qian PY. 2003. Effect of temperature, salinity and delayed attachment on development of the solitary ascidian Styela plicata (Lesueur). J Exp Mar Biol Ecol 290, 133-146. https://doi.org/10.1016/S0022-0981(03)00071-6
  34. Tsuchiya Y and Suzuki Y. 1960. Biochemical studies of the ascidian, Cynthia roretzi v. Drasche IV. Carotenoids in test. Tohoku J Argic Res 10, 397-407.
  35. Yen GC, Hung YL and Hsieh CL. 2000. Protective effect of extracts of Mesona-procumbens Hemsl. on DNA damage in human lymphocytes exposed to hydrogen peroxide and UV irradiation. Food Chem Toxicol 38, 747-754. https://doi.org/10.1016/S0278-6915(00)00069-7