Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Antimicrobial Activity of Brown Alga Eisenia bicyclis against Methicillin-resistant Staphylococcus aureus

  • Eom, Sung-Hwan (Department of Food Science and Technology, Pukyong National University) ;
  • Park, Jae-Hong (Department of Food Science and Technology, Pukyong National University) ;
  • Yu, Dae-Ung (Department of Food Science and Technology, Pukyong National University) ;
  • Choi, Ji-Il (Department of Food Science and Technology, Pukyong National University) ;
  • Choi, Jong-Duck (Department of Seafood Science and Technology, Gyeongsang National University) ;
  • Lee, Myung-Suk (Department of Microbiology, Pukyong National University) ;
  • Kim, Young-Mog (Department of Food Science and Technology, Pukyong National University)
  • Received : 2011.08.17
  • Accepted : 2011.10.28
  • Published : 2011.12.31
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Abstract

We screened for antibacterial substances against methicillin-resistant Staphylococcus aureus (MRSA). Methanolic extract of Eisenia bicyclis exhibited anti-MRSA activity according to a disk diffusion assay. To identify the active compound(s), the methanolic extract was further fractionated using hexane, dichloromethane, ethyl acetate, and n-butanol. The ethyl acetate-soluble fraction showed both the greatest anti-MRSA activity and the highest polyphenol content. The minimum inhibitory concentrations of the ethyl acetate fraction ranged from 32 to 64 ${\mu}g$ per mL against methicillin-susceptible S. aureus and MRSA strains. High-performance liquid chromatography analysis revealed that both the methanolic extract and the ethyl acetate soluble fraction contained sizeable quantities of dieckol, which is a known anti-MRSA compound. Thus, these data strongly suggest that the anti-MRSA activity of E. bicyclis may be mediated by phlorotannins such as dieckol.

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References

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