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Inhibitory Effect of Angelica keiskei Extracts on Melanogenesis

신선초 추출물의 멜라닌 생성 억제활성

  • Son, Hyeong-U (Department of Food Science & Biotechnology, Kyungpook National University) ;
  • Nam, Dong-Yoon (Department of Food Science & Biotechnology, Kyungpook National University) ;
  • Kim, Min-Ah (Department of Food Science & Biotechnology, Kyungpook National University) ;
  • Cha, Yong-Su (Department of Food Science & Biotechnology, Kyungpook National University) ;
  • Kim, Jong-Myung (Bion Co., Ltd.) ;
  • Shin, Yong-Kyu (Bion Co., Ltd.) ;
  • Lee, Sang-Han (Department of Food Science & Biotechnology, Kyungpook National University)
  • Received : 2011.06.30
  • Accepted : 2011.11.25
  • Published : 2011.12.30

Abstract

Angelica keiskei is a perennial herb belonging to the Umbelliferae family. In this study, the whitening effect of A. keiskei extracts was examined through melanogenesis and tyrosinase inhibitory assays. The ethanol extract (50%) significantly inhibited tyrosinase in a concentration-dependent manner. RT-PCR revealed that the extract exhibited decreased expression of tyrosinase, tyrosinase-related protein-1, tyrosinase-related protein-2, and melanocyte-inducing transcription factor. These results suggest that the extract can be used as an ingredient for the development of cosmeceuticals.

신선초 추출물의 미백활성을 확인하고 이의 소재를 개발하기 위하여, tyrosinase 저해 활성 및 B16F10 세포주를 이용한 melanogenesis 저해능을 확인하였다. 그 결과, 신선초 50% 에탄올 추출물에서 B16F10 melanoma 세포의 멜라닌 생성을 억제하였다. Melanogenesis 억제 활성에 관련이 있는 분자마커의 확인을 위하여 RT-PCR에 의한 mRNA 발현 수준을 검토한 결과, 농도의존적으로 TYR, TYRP-1. TYRP-2의 발현이 억제되었으며 MITF 전사인자의 발현도 감소시켰다. 본 추출물은 독성이 없으므로, 향후 신선초 추출물에서의 미백활성을 나타내는 단일물질을 분리/조제에 응용한다면 cosmeceutical의 소재로 개발이 기대된다.

Keywords

References

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