The Korean Journal of Pesticide Science (농약과학회지)

The Korean Society of Pesticide Science (한국농약과학회)
권호 표지

Method Validation for Monitoring of Agricultural Worker Exposure to Insecticide Fenthion

살충제 Fenthion에 대한 농작업자 노출 측정을 위한 분석/시험방법 검증

  • Kim, Eun-Hye (Department of Agricultural Biotechnology, Seoul National University) ;
  • Lee, Hye-Ri (Department of Agricultural Biotechnology, Seoul National University) ;
  • Choi, Hoon (National Institute of Food and Drug Safety Evaluation) ;
  • Moon, Joon-Kwan (School of Plant, Life and Environmental Sciences, Hankyong National University) ;
  • Hong, Soon-Sung (National Institute of Agricultural Science and Technology) ;
  • Jeong, Mi-Hye (National Institute of Agricultural Science and Technology) ;
  • Park, Kyung-Hun (National Institute of Agricultural Science and Technology) ;
  • Lee, Hyo-Min (Risk Information Division, Risk Prevention Policy Bureau, Korea Food and Drug Administration) ;
  • An, Xue Hua (Zhejiang Academy of Agricultural Sciences) ;
  • Kim, Jeong-Han (Department of Agricultural Biotechnology, Seoul National University)
  • 김은혜 (서울대학교 농생명공학부) ;
  • 이혜리 (서울대학교 농생명공학부) ;
  • 최훈 (식품의약품안전청 식품의약품평가원) ;
  • 문준관 (한경대학교 식물생명환경과학부) ;
  • 홍순성 (농촌진흥청 농업과학기술원) ;
  • 정미혜 (농촌진흥청 농업과학기술원) ;
  • 박경훈 (농촌진흥청 농업과학기술원) ;
  • 이효민 (식품의약품안전청 위해예방정책국) ;
  • 안설화 (절강성농과원) ;
  • 김정한 (서울대학교 농생명공학부)
  • Received : 2011.09.27
  • Accepted : 2011.10.23
  • Published : 2011.12.31
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Abstract

Exposure measurement of agricultural worker to pesticide is one of important part of health risk assessment of pesticide. Therefore exposure matrices, apparatus, instruments and methods must be validated in advance to field experiment. In this study, method validation with an organophosphorus insecticide fenthion was carried out for exposure monitoring of agricultural worker. LOD and LOQ were 0.01 and 0.05 ng, respectively. Calibration curve linearity ($R^2$ > 0.999) and reproducibility (C.V. < 3%) were also excellent. Recovery at LOQ, 10LOQ and 100LOQ levels from gloves, socks, mask, patch, solid sorbent, glass fiber filter was 76~113% (C.V. < 3%). Trapping efficiency was 95~105% while no breakthrough was observed. Method validation for the exposure monitoring was established successfully through several experiments. Such method validation can be usually performed in laboratory and not much different for each pesticide so that, this techniques will be applied widely in research for pesticide exposure monitoring by combination with body surface area and respiration rates.

농약을 살포하는 농작업자의 농약 노출 측정은 농약의 건강 위해성 평가에서 중요한 부분을 차지하기 때문에 정량적이고 신뢰성 있는 노출량의 측정을 위해서 노출 측정에 사용되는 재료와 기기/기구 및 방법은 포장 노출 시험 전에 미리 검증해야 한다. 본 연구는 유기인계 농약 중에서 살충제 fenthion을 대상으로 농작업자의 노출량 측정을 위한 분석/시험방법의 검증을 수행하였다. LOD는 0.01 ng, LOQ는 0.05 ng로 설정하였고, 표준 검량선의 직선성($R^2$ > 0.999)과 분석재현성(C.V. < 3%)은 매우 우수하였다. 또한 3수준(LOQ, 10LOQ, 100LOQ)으로 수행한 노출 시료(장갑, 양말, 마스크, 패치, 고체흡착제, 유리섬유필터) 중 fenthion의 회수율은 76~113%(C.V. < 3%)이었고, 호흡 노출 실험의 검증 중에서 포집효율은 95~105%이었다. 파과실험 결과 fenthion은 1차 고체흡착제 부분에만 존재하였다. 이와 같은 분석/실험법의 검증을 통하여 실제 포장 실험에서 유래한 노출 시료들을 신뢰성 있게 분석할 수 있는 방법을 확인/검증/확립하였다. 이러한 분석/시험법 검증은 실험실 수준에서 수행할 수 있고, 농약의 종류에 따라서도 크게 다르지 않기 때문에 신체 부위별 평균 체표면적이나, 평균 호흡량과의 조합을 통해서 농약 노출 연구에 적극적으로 활용될 것으로 판단한다.

Keywords

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