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Rearing Method for Ascotis selenaria (Lepidoptera: Geometridae) using an Artificial Diet

인공사료를 이용한 네눈쑥가지나방(Ascotis selenaria)(나비목: 자나방과) 실내 사육법

  • Choi, Kyung-San (Agricultural Research Center for Climate Change, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Park, Young-Mi (Jungbu Regional Office, National Plant Quarantine Service) ;
  • Kim, Dong-Soon (Majors in Plant Resource Sciences & Environment, Jeju National University)
  • 최경산 (농촌진흥청 국립원예특작과학원 온난화대응농업연구센터) ;
  • 박영미 (국립식물검역원 중부지원) ;
  • 김동순 (제주대학교 생명자원과학대학 식물자원환경)
  • Received : 2011.02.01
  • Accepted : 2011.03.15
  • Published : 2011.03.30

Abstract

This study was conducted to develop an artificial diet for the mugwort looper, Ascotis selenaria (Lepidoptera: Geometridae), which is an insect pest to leaves of citrus (Citrus unshiu). Corn and soybean powder were selected as main nutrient sources for larvae of A. selenaria after several diets consisted of wheat germ, corn, kidney bean and/or soybean were tested for larval development and survival. A higher amount of the main nutrients in the diet increased the larval survivorship. Addition of yeast and cholesterol in diet increased the larval survivorship. Finally the composition of diet was decided as followings; corn 100 g, soybean 100 g agar 25 g, Brewers' yeast 30 g, cholesterol 0.5 g, Vanderzant vitamin mixture 2 g, Wesson's salt mixture 2 g, sorbic acid 2 g, ascorbic acid 2 g, and methyl-4-hydroxybenzoate 2.5 g, and distilled water 1 liter. Development periods of larvae and pupae, survival rate and fecundity of A. selenaria reared on the diet were not significantly different with those on the host plant, citrus leaves. Larvae of early instars were reared in a group, while larvae of later instars (5-6th) were reared individually. Adult mating was conducted in a plastic cage and an oilpaper covered with a gauze was provided as an oviposition site.

본 연구는 감귤에 발생하는 해충인 네눈쑥가지나방의 실내 사육용 인공사료를 개발하고자 수행하였다. 인공사료의 주 재료로 옥수수와 메주콩을 조합한 사료에서만 정상적으로 발육하였으며, 옥수수와 메주콩의 전체 함량이 높은 사료에서 유충 생존성이 높았다. 효모와 콜레스테롤을 인공사료에 첨가하였을 때, 유충 발육기간은 짧아지고, 생존율은 높은 것으로 나타났다. 발육기간, 누적 생존율, 산란량에 차이는 인공사료와 감귤 잎을 먹은 개체들 간에 없었다. 최종적으로 네눈쑥가지나방 사육에 최적인 인공사료 조성은 물 1 L당 한천 25 g, 옥수수 100 g, 메주콩 100 g, 효모 30 g, 콜레스테롤 0.5 g, 비타민혼합물(Vanderzant vitamin mix.) 2 g, 염혼합물(Wesson's salt mix.) 2 g, 소르브산 2 g, 메틸파라벤(methyl-4-hydroxybenzoate) 2.5 g, 아스코르브산 2 g이었다. 사육법으로 유충은 플라스틱 원형용기(${\varphi}90{\times}40$ mm) 안에서, 갓 부화 유충부터 4령까지는 40마리씩 집단으로, 5령과 6령은 개체별 사육하였다. 성충들에는 10% 설탕물을 먹이로 하여 플라스틱 상자 안($25{\times}25{\times}25$ cm)에서 암컷 3마리와 수컷 5~6마리가 교미하도록 했고, 난괴는 거즈(눈 크기 1 mm)를 붙인 기름종이에 받았다. 기타 인공사료로 사육한 네눈쑥가지나방의 발육단계별 형태적 특징에 대하여 기술하였다.

Keywords

References

  1. Adkisson, P.L., E.S. Vanderzant, D.L. Bull and W.E. Allison. 1960. A wheat germ medium for rearing the pink bollworm. J. Econ. Entomol. 53: 759-762. https://doi.org/10.1093/jee/53.5.759
  2. Ando, T.,K. Ohtani, M. Yamamoto, T. Miyamoto, X. Qin and Witjaksono. 1997. Sex pheromone of Japanese giant looper, Ascotis selenaria cretacea: Identification and field tests. J. Chem. Ecol. 23: 2413-2423. https://doi.org/10.1023/B:JOEC.0000006683.58028.1e
  3. Ave, D.A. 1995. Stimulation of feeding: Insect control agents. pp. 345-363. In Regulatory mechanisms in insect feeding, eds. by R.F. Chapman and G. de Boer. Chapman & Hall, New York.
  4. Cohen, A.C. 2003. Insect diets: science and technology. 324pp, CRC press, Boca Raton.
  5. Dougherty, E.C. 1959. Introduction to axenic culture of invertebrate metazoan: a goal. Ann. N.Y. Acad. Sci. 77: 27-54.
  6. Fraenkel, G. 1969. Evaluation of our thoughts on secondary plant substances. Ent. Exp. & Appl. 12: 473-486. https://doi.org/10.1111/j.1570-7458.1969.tb02546.x
  7. Hobson, R.P. 1935. On a fat-soluble growth factor required by blowfly larvae. II. Identity of the growth factor with cholesterol. Biochem. J. 29: 2023-2026.
  8. House, H.L. 1974. Nutrition. pp. 1-62. In The physiology of insecta, Vol. 5, eds. by M. Rockstein. 2nd ed., 648 pp. Academic Press, New York.
  9. Kim, S.S. and E.A. Beljaev. 2001. Family geometridae. Economic insects of Korea 8. Ins. Koreana Suppl. 15, 247 pp., Junghaeng-Sa, Seoul.
  10. Langsrud, O. 2004. Fisher's exact test (accessed in April, 2010 at http://www.langsrud.com/fisher.htm).
  11. SAS Institute. 2006. SAS enterprise guide 4.1 (4.1.0.471). SAS Institute Inc. Cary, NC, USA.
  12. Schoonhoven, L.M. 1972. Some aspects of host selection and feeding in phytophagous insects, pp. 557-566. In Insect and mite nutrition, eds. by J.G. Rodriguez. 702 pp. North-Holland, Amsterdam.
  13. Singh, A.K. 1999. Growth and induction in food consumption of Helicoverpa armigera Hbn. (Lep., Noctuidae) larvae on chickpea, soybean, and maize diets. J. Appl. Ent. 123: 335-339. https://doi.org/10.1046/j.1439-0418.1999.00349.x
  14. Singh, A.K. and H. Rembold. 1988. Developmental value of chickpea, Cicer arietinum, soybean, Glycine max and maize, Zea mays, flour for Heliothis armigera larvae. J. Appl. Ent. 106: 286-296. https://doi.org/10.1111/j.1439-0418.1988.tb00595.x
  15. Shorey, H.H. 1963. A simple artificial rearing medium for the cabbage looper. J. Econ. Entomol. 56: 536-537.
  16. Shorey, H.H. and R.L. Hale. 1965. Mass-rearing of the larvae of nine noctuid species on a simple artificial medium. J. Econ. Entomol. 58: 522-524. https://doi.org/10.1093/jee/58.3.522
  17. Vanderzant, E.S. 1974. Development, significant, and application of diets for insects. Annu. Rev. Entomol. 19: 139-160. https://doi.org/10.1146/annurev.en.19.010174.001035
  18. Wolf, W.J. and J.C. Cowan. 1971. Soybeans as a food source. Critical Rev. in Food Technol. 2: 81-158. https://doi.org/10.1080/10408397109527117

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