Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Biological Control of Phytophthora Blight and Anthracnose Disease in Red-pepper Using Bacillus subtilis S54

Bacillus subtilis S54 균주를 이용한 고추 역병과 탄저병의 생물학적 방제

  • Lee, Gun-Woong (Division of Biotechnology, Chonbuk National University) ;
  • Kim, Myung-Jun (Agricultural Technology Center, Imsil) ;
  • Park, Jun-Sik (Agricultural Technology Center, Imsil) ;
  • Chae, Jong-Chan (Division of Biotechnology, Chonbuk National University) ;
  • Soh, Byoung-Yul (Department of Life Science, Faculty of Natural Science, Seonam University) ;
  • Ju, Jae-Eun (Division of Biotechnology, Chonbuk National University) ;
  • Lee, Kui-Jae (Division of Biotechnology, Chonbuk National University)
  • Received : 2010.11.30
  • Accepted : 2011.03.05
  • Published : 2011.04.30
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Abstract

Phytophthora blight and anthracnose disease caused by Phytophthora capsici and Collectotrichum gloeosporioides are the most important devastating diseases of red pepper plants, worldwide. Five different bacterial isolates were isolated from the red pepper rhizosphere and non-rhizosphere soil and subsequently tested for antagonistic activity against P. capsisi and C. gloeosporioides. The area of the inhibition zone was taken as a measure for antagonistic activity. Among the 5 isolates tested, S54 exhibited a maximum antagonistic activity under in vitro and in vivo conditions. In greenhouse studies the isolate has successfully reduced the disease symptom. Protect value was 80.8% (Phytophthora blight) and 81.9% (Anthrancnose disease), whereas the infection rate of control plants was 21.3% and 23.2%. Based on the 16S rDNA sequence and API 50CHB Kit analysis the most effective isolate was identified as Bacillus subtilis. The results of the study indicate that the stratin S54 could be used as an potential biological control of Phytophthora blight and anthracnose disease of red pepper.

고추 역병과 탄저병은 Phytophthora capsici와 Colletotrichum gloeosporioides에 의해 발병되는 병으로 세계적으로 많은 피해를 입히는 중요한 병원균이다. 이러한 병원균의 방제를 위하여 근권과 비근권에서 각각의 병원균 생장을 억제하는 능력이 뛰어난 5균주를 선발하였다. 선발된 분리주 중 S54균주는 in vitro와 in vivo 조건 모두에서 가장뛰어나게 병원균을 억제하였다. 하우스 실험에서도 역병과 탄저병 모두 80% 이상의 방제력을 보여주었다. 또한 S54균주의 동정을 위하여 16S rDNA 부분의 염기서열의 분석과 API 50CHB Kit를 실시하였으며, 그 결과 Bacillus subtilis로 동정하였다. 이러한 결과를 바탕으로 S54는 고추 역병과 탄저병을 방제할 수 있는 생물학적 방제제로의 가능성을 보여주었다고 할 수 있다.

Keywords

References

  1. Abdul-Baki, A. A. and Anderson, J. D. 1973. Vigour determination in soyabean seed by multiple criteria. Crop Sci. 31: 630-633.
  2. Chiou, A. L. and Wu, W. S. 2001. Isolation, identification and evaluation of bacterial antagonists against Botrytis elliptica on Lily. J Phytopathol. 149: 319-324. https://doi.org/10.1046/j.1439-0434.2001.00627.x
  3. Cho, M. C., Chae, U., Cho, Y. S., Ko, K. D., Shin, Y. A., Kim, D.H. and Jung, J. W. 2005. The result of new pepper varieties evaluation at farmer's field. Korean J. Hort. Sci. Technol. (Suppl. I) 23: 38.
  4. Leoffler, W., Tschen, J. S., Venittanakom, N., Kugler, M., Knorpp,E., Hsieh, T. F. and Wu, T. G. 1986. Antifungal effects of bacilysin and fengycin from Bacillus subtilis F-29-3: a comparison with activaties of other Bacillus antibiotics. J. Phytopathol. 115: 204-213. https://doi.org/10.1111/j.1439-0434.1986.tb00878.x
  5. Paulitz, T. C. and Loper, J. E. 1991. Lack of a role for fluorescent siderophore production in the biological control of Phythium damping-off of cucumber by a strain of Pseudomonas putida. Phytopathology 81: 930-935. https://doi.org/10.1094/Phyto-81-930
  6. Watanabe, T., Oyanagi, W., Suzuki, K. and Tanaka, H. 1990.Chitinase system of Bacillus circulans WL-12 and importance of chitinase A1 in chitin degradation. J. Bacteriol. 172: 4017-4022. https://doi.org/10.1128/jb.172.7.4017-4022.1990
  7. Yoshida, S., Hiradate, S., Tsukamoto, T., Hatakeda, K. and Shirata, A. 2001. Antimicrobial activity of culture filtrate of Bacillus amyloliquefaciens RC-2 isolated from mulberry leaves. Phytopathology 91: 181-187 https://doi.org/10.1094/PHYTO.2001.91.2.181
  8. Yin, J. F., Zhang, W. H., Li, J. Q., Li, Y. H., Hou, H. L. and Zhou,X. Y. 2007. Screening and antagonistic mechanism of biocontrol agents against Phytophthora blight of pepper. Acta Phytopathologica Sinica. 37: 88-94.

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