Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Antibacterial Activity of Herbal Medicine Extracts against Edwardsiella tarda

어병세균 Edwardsiella tarda에 대한 한약재 추출물의 항균활성

  • Kim, Ah-Ra (Department of Life Science and Biotechnology, Dongeui University) ;
  • Kim, Do-Kyun (Department of Life Science and Biotechnology, Dongeui University) ;
  • Byun, Tea-Hwan (Department of Life Science and Biotechnology, Dongeui University) ;
  • Jo, Eun-Jee (Department of Life Science and Biotechnology, Dongeui University) ;
  • Lee, Eun-Woo (Department of Life Science and Biotechnology, Dongeui University) ;
  • Kwon, Hyun-Ju (Department of Life Science and Biotechnology, Dongeui University) ;
  • Kim, Byung-Woo (Department of Life Science and Biotechnology, Dongeui University) ;
  • Kim, Tae-Hoon (Department of Herbal Medicinal Pharmacology, Daegu Haany University) ;
  • Lee, Kyung-Bon (Departments of Animal Science and Physiology, Michigan State University) ;
  • Kim, Young-Man (Department of Food Science and Nutrition, Dongeui University)
  • 김아라 (동의대학교 생명응용학과) ;
  • 김도균 (동의대학교 생명응용학과) ;
  • 변태환 (동의대학교 생명응용학과) ;
  • 조은지 (동의대학교 생명응용학과) ;
  • 이은우 (동의대학교 생명응용학과) ;
  • 권현주 (동의대학교 생명응용학과) ;
  • 김병우 (동의대학교 생명응용학과) ;
  • 김태훈 (대구한의대학교 한약재약리학과) ;
  • 이경본 (미시간주립대학교 동물과학.생리학과) ;
  • 김영만 (동의대학교 식품영양학과)
  • Received : 2010.08.30
  • Accepted : 2011.01.21
  • Published : 2011.02.28
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Abstract

The methanol extracts of 19 commercial herb medicines was analyzed to antibacterial activities against Edwardsiella tarda, causing several fish diseases. Rhus javanica showed most strong antibacterial activity against E. tarda and Escherichia coli. Methanol extract of R. javanica was further extracted using several organic solvents having different polarity. Extract from ethyl acetate fraction showed strong activity against E. tarda as well as E. coli. Minimal inhibitory concentration, MIC of R. javanica extract was measured and resulted showing $64\;{\mu}g/m{\ell}$ for E. tarda and $256\;{\mu}g/m{\ell}$ for E. coli. It is needed that, from these results, further purification and isolation of reposible compound of these activities and further study on the synergy effect using combination with antibiotics against pathogenic bacteria.

시판 19종 한약재 methanol 추출물의 어병세균 Edwardsiella tarda에 대한 항균활성을 측정한 결과, 오배자 추출물에서 가장 강한 활성이 나타났다. 오배자 methanol 추출물은 용매 극성에 따라 분획하였으며, 각 분획물의 항균활성 측정 결과 ethyl acetate 층에서 가장 강한 항균 활성이 나타났다. 이는 E. coli와E. tarda 모두에 대해 유사한 결과를 보였다. 오배자 추출물의 최소생육저해농도(MIC)를 측정한 결과 $64\;{\mu}g$/mL에서 E. tarda의 생육을, $256\;{\mu}g$/mL에서 E. coli의 생육을 저해할 수 있는 것으로 나타났다. 이 결과는 활성물질이 정제되어 단일 물질화 하였을 경우에는 상당히 낮은 농도에서도E. tarda에 대한 저해능을 기대할 수 있을 것이다. 본 실험 결과에서 나타난E. coli와E. tarda에 대해 강한 효능을 나타내는 항균활성물질을 분리 동정 중에 있으며, 상용되고 있는 항생제와의 병용투여 효과 및 조건 등에 대한 연구가 지속적으로 진행되어야 한다.

Keywords

References

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