Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
권호 표지
DOI QR코드

DOI QR Code

Inhibitory Effects of Ginseng Extracts on Histamine-release from Rat's Mast Cell

인삼추출물의 랫트 비만세포 히스타민 유리 억제 효과

  • Park, Kwang-Hyun (Department of Biochemistry, Chonbuk National University Medical School) ;
  • Kim, Young-Seon (Department of Medicated Diet & Food Technology, Jeonnam Provincial College) ;
  • Jeong, Jae-Hun (Department of Medicated Diet & Food Technology, Jeonnam Provincial College)
  • 박광현 (전북대학교 의학전문대학원 생화학교실) ;
  • 김영선 (전남도립대학 약선식품가공과) ;
  • 정재훈 (전남도립대학 약선식품가공과)
  • Received : 2010.12.28
  • Accepted : 2011.02.15
  • Published : 2011.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

We investigated inhibitory effects of ginseng extracts against compound 48/80-induced responses in rat peritoneal mast cells. Initially, we optimized extraction condition with various temperature and time for recovery of high saponin contents in extracts. Using a primary rat peritoneal mast cells, we examined whether ginseng extracts inhibit compound 48/80-induced histamine release form rat mast cells. High red ginseng-spercific saponin containing extracts were recovered at $85^{\circ}C$ for 48 hr, and had no cytotoxicity with relatively high dose of extracts on rat peritoneal mast cells(<0.5 mg/ml). For examine of ameliorate effects of mast cells responses by ginseng extract, we pre-treated the extracts or saline to mast cells and treated compound 48/80. In results, compound 48/80 treatment was increased histamine release (approximately 30%) from mast cells than normal group, whereas ginseng treatment was completely inhibited histamine release. These results suggested that ginseng extracts inhibits the compound 48/80-induced mast cell activation, and ginseng extracts is a candidate for effective therapeutic tools of allergic diseases.

수삼 추출물에서 홍삼특이 사포닌 성분의 함량을 증대시키고자 추출온도 및 시간에 따른 사포닌 성분별 함량 변화를 조사하였다. 수삼을 $85^{\circ}C$에서 48시간 추출 시 총 사포닌 함량이 23.5 mg/g(D.W.)로 가장 많았으며, 본 조건에서 추출한 인삼추출물의 항알러지 기능성을 검토하고자 랫트 비만세포에서 compound 48/80로 유도된 히스타민 유리 작용을 억제하는 인삼추출물의 효능을 분석하였다. 인삼추출물의 비만세포에 대한 세포 독성 시험 결과 각 추출물은 수시간 노출시 높은 세포 생존율을 보이고 있었고 비교적 고농도인 0.5 mg/ml 처리군에서도 80% 이상의 세포 생존율을 보였다. 인삼추출물의 히스타민 유리 억제 효과를 조사하기 위해 랫트 비만세포에 대표적인 탈과립 유도제인 compound 48/80을 단독 또는 각 추출물을 동시에 처리한 후 얻어진 상청액의 히스타민의 정량을 실시한 결과 비만세포는 compound 48/80의 처리 조건에서 대조군에 비하여 30% 가량의 히스타민 유리량이 증가하였는데, 인삼추출물 처리 시 히스타민 유리 수준이 정상 수준에 가까이 감소하는 것을 확인하였다. 이는 인삼추출물의 항 알러지 목적으로의 이용 가능성을 제시하고 있다.

Keywords

References

  1. Ackermann, L. and I.T. Harvima. 1998. Mast cells of psoriatic and atopic dermatitis skin are positive for TNF- α and their degranulation is associated with expression of ICAM-1 in the epidermis. Arch. Dermatol. Res. 290: 353-359. https://doi.org/10.1007/s004030050317
  2. Alfonso, A., A.G. Cabado, M.R. Vieytes and L.M. Botana. 2000. Functional compartments in rat mast cells for cAMP and calcium on histamine release. Cell Signal 12: 343-350. https://doi.org/10.1016/S0898-6568(00)00070-X
  3. Ando, T., O. Tanaka and S. Shibata. 1971. Comparative studies on the saponins and sapogenins of ginseng and related crude drugs. Syoyakugaku Zasshi. 25:28-32.
  4. Andrew, D., and A.D. Craig. 2001. Spinothalamic lamina I neurons selectively sensitive to histamine: a central neural pathway for itch. Nature Neuroscience. 4(1):72-77. https://doi.org/10.1038/82924
  5. Assanasen, P., and R.M. Naclerio. 2000. Antiallergic effects of H1-receptor antagonists. Allergy 55(Suppl 64):17-27.
  6. Bae, E.A., M.J. Han, Y.W. Shin and D.H. Kim. 2005. Antiallergic and antipsoriatic effects of Korean red ginseng. J. Ginseng Res. 29:80-85. https://doi.org/10.5142/JGR.2005.29.2.080
  7. Chai, O.H., M.S. Lee, E.H. Han, H.T. Kim and C.H. Song. 2005. Inhibitory effects of Morus alba on compound 48/80-induced anaphylactic reactions and anti-chicken gamma globulin IgE- mediated mast cell activation. Biol. Pharm. Bull. 28(10):1852-1858. https://doi.org/10.1248/bpb.28.1852
  8. Homey, B., M. Steinhoff, T. Ruzicka and D.Y. Leung. 2006. Cytokines and chemokines orchestrate atopic skin inflammation. J. Allergy Clin. Immunol. 118:178-189. https://doi.org/10.1016/j.jaci.2006.03.047
  9. Huo, Y., L. Zhao, M. Utsuyama and K. Hirokawa. 1998. Effects of ginseng saponin on the immunity of young and old mice. Proceeding of the 7th Intenational Symposium on Ginseng, The Kor. Soc. of Gingeng. p. 281-288.
  10. In, J.G., B.S. Lee, E.J. Kim, M.H. Park and D.C. Yang. 2006a. Increase of functional saponin by acidic treatment and temperature of red ginseng extracts. Kor. J. Plant Res. 19(1):139-143.
  11. In, J.G., E.J. Han, B.S. Lee, M.H. Park and D.C. Yang. 2006b. Saponin analysis and red ginseng production using the simplified method of Korean ginseng (Panax ginseng C.A. Meyer). Kor. J. Plant Res. 19(1):133-138 (in Korean).
  12. Jeong, H.J., J.M. Yi, S.H. Hong, J.Y. Kong, K.S. Kim, J.H. Won, K.H. Cho, T.Y. Shin, H.S. Kim and H.M Kim. 2002. Effect of Sinpo-Tang on the mast cell-mediated anaphylactic reactions, Pharmacological Res. 46(5):453-458. https://doi.org/10.1016/S1043661802002037
  13. Joo, C.N. 1990. Some physiological and biochemical aspects of saponin fraction of Panax ginseng C.A. Meyer. Kor. J. Ginseng. Sci. 14:143-156.
  14. Jung, N.P., S.O. Song and S.U. Choi. 2000. Cytotoxicity of white and red ginseng against cancer cells and their effects on the cell cycle. J. Ginseng Res. 24:183-187.
  15. Kenarova, B., H. Neychev, C. Hadjiivnova and V.D. Petkov. 1990. Immunomodulating activity of ginsenosides Rg1 from Panax ginseng. Japan J. Pharmacol. 54:447-454. https://doi.org/10.1254/jjp.54.447
  16. Kim, J.S., M.R. Chae, K. Chang, K.H. Park, H.W. Rho, B.H. Park, J.W. Park and H.R. Kim. 1998. Cytotoxicity of Vibrio vulnificus cytolysin on rat peritoneal mast cells. Microbiol Immunol. 42(12):837-43. https://doi.org/10.1111/j.1348-0421.1998.tb02359.x
  17. Kitagawa, I. 1987. Chemical studies on the crude drug processing. V. On the constituents of ginseng radix rubra (II): comparison og the constituents of white and red ginseng prepared form the same Panax ginseng root. Yakugaku Zassi 107:495-505. https://doi.org/10.1248/yakushi1947.107.7_495
  18. Kumar, A. 1993. Chemopreventive action of ginseng on DMBA-induced papilloma genesis in the skin of mice. Proc 6th Int'l Ginseng Symp Korea Ginseng Tobacco Research Institute. p. 66-73.
  19. Li, X.G. 1992. Studies on the transforming mechanism of amino acid cimponents in ginseng in the course of ginseng processing. Kor. J. Ginseng Sci. 16:64-67.
  20. Liao, B.C., R.C. Hou, I.S. Wang and K.C. Jeng. 2006. Enhancement of the release of inflammatory mediators by substance P in rat basophilic leukemia RBL-2H3 cells. J. Biomed. Sci. 13(5):613-619. https://doi.org/10.1007/s11373-006-9099-1
  21. Matsuda, H., N. Watanabe, G.P. Geba, J. Sperl, M. Tsudzuki, J. Hiroi, M. Matsumoto, H. Ushio, S. Saito, P.W. Askenase and C. Ra. 1997. Development of atopic dermatitis-like skin lesion with IgE hyperproduction in NC/Nga mice. Int. Immunol. 9:461-466. https://doi.org/10.1093/intimm/9.3.461
  22. Moon, P.D., H.J. Na and H.M. Kim. 2003. Action of enzyme food, Green Life Enzyme of systemic and local ana-phylaxis. Orient Pharm Exp Med, 3:46-50. https://doi.org/10.3742/OPEM.2003.3.1.046
  23. Mosmann, T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods. 65(1-2):55-63. https://doi.org/10.1016/0022-1759(83)90303-4
  24. Nakazato, J., M. Kishida, R. Kuroiwa, J. Fujiwara, M. Shimoda and N. Shinomiya. 2008. Serum levels of Th2 chemokines, CCL17, CCL22, and CCL27, were the important markers of severity in infantile atopic dermatitis. Pediatr. Allergy Immunol. 19:605-613.
  25. Nam, K.Y. 2005. The comparative understanding between red ginseng and white ginsengs processed ginsengs (Panax ginseng C.A. Meyer). J. Ginseng Res. 29:1-18. https://doi.org/10.5142/JGR.2005.29.1.001
  26. Noh, S.U., E.A. Cho, H.O. Kim and Y.M. Park. 2008. Epigallocatechin-3-gallate improves dermatophagoides pteronissinus extract-induced atopic dermatitis-like skin lesions in NC/Nga mice by suppressing macrophage migration inhibitory factor. Int. Immunopharmacol. 8: 1172-1182. https://doi.org/10.1016/j.intimp.2008.04.002
  27. Okano, Y., K. Yamada, H. Takagi and Y. Nozawa. 1986. Bibasic effect of phorbol myristate acetate on histamine secretion induced by compound 48/80 in rat peritoneal mast cells. Biochem Biophys Res Commun 137:1112-1118. https://doi.org/10.1016/0006-291X(86)90340-2
  28. Okuda, H. and S.D. Lee. 1990. Biological activities of non-saponin compounds isolated from Korean red ginseng. Proc Int'l Symp on Korean ginseng, The Society for Korean Ginseng, Seoul, Korea. p. 15-19.
  29. Park, E.K., M.K. Choo, E.J. Kim, M.J. Han and D.H. Kim. 2003. Antiallergic activity of ginsenoside Rh2. Biol. Pharm. Bull. 26:1581-1584. https://doi.org/10.1248/bpb.26.1581
  30. Park, E.K., M.K. Choo, M.J. Han and D.H. Kim. 2004. Ginsenoside Rh1 possesses antiallergic and anti-inflammatory activities. Int. Arch. Allergy Immunol. 133:113-120. https://doi.org/10.1159/000076383
  31. Quan, L.H., Z. Liang, H.B. Kim, S.H. Kim, S.Y. Kim, Y.D. Noh and D.C. Yang. 2008. Conversion of ginsenoside Rd to compound K by crude enzymes extracted from Lactobacilus brevis LH8. J. Ginseng Res. 32:226-231. https://doi.org/10.5142/JGR.2008.32.3.226
  32. Ro, J.Y., Y.S. Ahn and K.H. Kim. 1998. Inhibitory effect of ginsenoside on the mediator release in the guinea pig lung mast cells activated by specific antigen-antibody reactions. Int. J. Immunopharmacol. 20(11):625-641. https://doi.org/10.1016/S0192-0561(98)00062-9
  33. Rothschild, A.M. 1970. Mechanisms of histamine release by compound 48/80. Br. J. Pharmacol. 38(1):253-262. https://doi.org/10.1111/j.1476-5381.1970.tb10354.x
  34. Royer, B., S. Varadaradjalou, P. Saas, A.C. Gabiot, B. Kantelip, F. Féger, J.J. Guillosson, J.P. Kantelip and M. Arock. 2001. Autocrine regulation of cord blood-derived human mast cell activation by IL-10. J. Allergy Clin. Immunol. 108(1):80-6. https://doi.org/10.1067/mai.2001.115753
  35. Shin, Y.W. and D.H. Kim. 2005. Antipruritic effect of ginsenoside rb1 and compound k in scratching behavior mouse models. J. Pharmacol Sci. 99(1):83-88. https://doi.org/10.1254/jphs.FP0050260
  36. Singh, V.K., S.S. Agarwal and B.M. Gupta. 1984. Immunomodulatory activity of Panax ginseng extract. Proc 4th Int'l Ginseng Symp. Kor. Ginseng & Tobacco Res. Institute. p. 225-232.
  37. Trinh, H.T., Y.W. Shin, S.J. Han, M.J. Han and D.H. Kim. 2008. Evaluation of antipruritic effects of red ginseng and its ingredients in mice. Planta Med. 74(3);210-214. https://doi.org/10.1055/s-2008-1034313
  38. Yamada, A., H. Yano, Y. Takao, T. Ono, T. Matsumoto and K. Itoh. 2006. Nonmutated Self-Antigen-Derived Cancer Vaccine Peptides Elicit an IgE-Independent but Mast Cell-Dependent Immediate-Type Skin Reaction without Systemic Anaphylaxis. J. Immunol. 176:857-863. https://doi.org/10.4049/jimmunol.176.2.857

Cited by

  1. Anti-stress and Anti-histamine Effects of Phragmites communis, Salicornia herbacea, and Prunus mume vol.14, pp.2, 2016, https://doi.org/10.20402/ajbc.2016.0029
  2. Inhibition Activity of Plants on IgE-mediated Degranulation of RBL-2H3 Cells vol.28, pp.6, 2015, https://doi.org/10.7732/kjpr.2015.28.6.718
  3. Immunomodulating Effects of Red Ginseng on the Regulation of Cytokine Release in vivo vol.25, pp.5, 2012, https://doi.org/10.7732/kjpr.2012.25.5.578
  4. Effects of pectin lyase-modified red ginseng extracts in high-fat diet-fed obese mice vol.30, pp.4, 2014, https://doi.org/10.5625/lar.2014.30.4.151
  5. Commercialization Strategy Based on Analysis of Domestic Consumers’ Preference and Awareness on South and North Korean Regional Cuisine : Research on Consumers in Seoul and Gyeonggi Province vol.32, pp.6, 2016, https://doi.org/10.9724/kfcs.2016.32.6.734
  6. 홍마늘 조성물의 항산화 및 항염증 효과 vol.24, pp.3, 2011, https://doi.org/10.11002/kjfp.2017.24.3.446
  7. 인삼, 산양산삼 및 홍삼 추출물의 항비만 효과 vol.27, pp.4, 2011, https://doi.org/10.14374/hfs.2019.27.4.269