Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Effects of Nrogen Form and Light Conditions on the Nitrate Reductase Activity of Ulva pertusa (Chlorophyta) and Ecklonia cava (Phaeophyta)

구멍갈파래 (Ulva pertusa Kjellman)와 감태 (Ecklonia cava Kjellman)의 질산환원요소 활성에 미치는 질소원 형태와 빛의 효과

  • Hwang, Jae-Ran (Marine Environmental Research Division, National Fisheries Research and Development Institute) ;
  • Kang, Yun-Hee (Marine Research Institute, Pusan National University) ;
  • Oak, Jung-Hyun (Marine Research Institute, Pusan National University) ;
  • Lee, Sang-Rae (Marine Research Institute, Pusan National University) ;
  • Chung, Ik-Kyo (Division of Earth Environmental System, Pusan National University)
  • 황재란 (국립수산과학원 어장환경과) ;
  • 강윤희 (부산대학교 해양연구소) ;
  • 옥정현 (부산대학교 해양연구소) ;
  • 이상래 (부산대학교 해양연구소) ;
  • 정익교 (부산대학교 지구환경시스템학부)
  • Received : 2010.10.26
  • Accepted : 2011.02.10
  • Published : 2011.02.28
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Abstract

Nitrate reductase (NR) is activated by nitrogen sources (${NO_3}^-$ and ${NH_4}^+$) and irradiance. This study investigated the effects of these factors on the NR activity of Ulva pertusa (Chlorophyta) and Ecklonia cava (Phaeophyta). In addition, the ammonium (${NH_4}^+$) and nitrate (${NO_3}^-$) uptake rates of the two species were examined. U. pertusa took up most of the ${NO_3}^-$ and ${NH_4}^+$ in the medium during a 3hour incubation, while E. cava had a relatively high uptake rate after 3 hours. The NR activities of the two species were affected by the nitrogen source and irradiance and were highest when they were exposed to ${NO_3}^-$-rich medium and high irradiance. However, the patterns of NR activity differed between the two species. In ${NO_3}^-$-rich medium and high irradiance, U. pertusa achieved the highest NR activity ($2.01{\pm}0.07\;{\mu}mol$ ${NO_2}^-$ $g^{-1}$ DW $h^{-1}$) within the first 3 hours and then this activity decreased drastically. By contrast, the NR activity of E. cava ($0.36{\pm}0.04\;{\mu}mol$ ${NO_2}^-$ $g^{-1}$ DW $h^{-1}$) was constant for 12 hours. When exposed to darkness, the NR activity of U. pertusa decreased dramatically, while that of E. cava increased gradually for 12 hours. Therefore, E. cava is able to maintain NR activity during the dark because of its adequate carbohydrate reserves and substrate.

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References

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